Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08650

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08640 and BWI76_RS08645 are separated by 143 nucleotidesBWI76_RS08645 and BWI76_RS08650 overlap by 1 nucleotidesBWI76_RS08650 and BWI76_RS08655 are separated by 2 nucleotides BWI76_RS08640: BWI76_RS08640 - multidrug efflux pump-associated protein, AcrZ family, at 1,823,030 to 1,823,179 _RS08640 BWI76_RS08645: BWI76_RS08645 - molybdate ABC transporter substrate-binding protein, at 1,823,323 to 1,824,096 _RS08645 BWI76_RS08650: BWI76_RS08650 - molybdate ABC transporter permease, at 1,824,096 to 1,824,785 _RS08650 BWI76_RS08655: BWI76_RS08655 - molybdenum ABC transporter ATP-binding protein, at 1,824,788 to 1,825,846 _RS08655 Position (kb) 1824 1825Strain fitness (log2 ratio) -1 0 1at 1823.226 kb on + strandat 1823.234 kb on - strandat 1823.336 kb on + strandat 1823.433 kb on + strand, within BWI76_RS08645at 1823.441 kb on - strand, within BWI76_RS08645at 1823.906 kb on + strand, within BWI76_RS08645at 1824.126 kb on + strandat 1824.170 kb on - strand, within BWI76_RS08650at 1824.284 kb on - strand, within BWI76_RS08650at 1824.809 kb on - strandat 1824.893 kb on + strandat 1824.907 kb on + strand, within BWI76_RS08655at 1825.150 kb on + strand, within BWI76_RS08655at 1825.464 kb on - strand, within BWI76_RS08655

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
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1,823,226 + +0.3
1,823,234 - -0.8
1,823,336 + +0.2
1,823,433 + BWI76_RS08645 0.14 -0.5
1,823,441 - BWI76_RS08645 0.15 +0.5
1,823,906 + BWI76_RS08645 0.75 +0.8
1,824,126 + +0.3
1,824,170 - BWI76_RS08650 0.11 +1.4
1,824,284 - BWI76_RS08650 0.27 +0.4
1,824,809 - +0.5
1,824,893 + +1.4
1,824,907 + BWI76_RS08655 0.11 +1.3
1,825,150 + BWI76_RS08655 0.34 +0.6
1,825,464 - BWI76_RS08655 0.64 -0.4

Or see this region's nucleotide sequence