Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08315 and BWI76_RS08320 are separated by 21 nucleotides BWI76_RS08320 and BWI76_RS08325 overlap by 1 nucleotides BWI76_RS08325 and BWI76_RS08330 are separated by 309 nucleotides BWI76_RS08330 and BWI76_RS08335 are separated by 202 nucleotides
BWI76_RS08315: BWI76_RS08315 - K+-transporting ATPase subunit B, at 1,759,594 to 1,761,642
_RS08315
BWI76_RS08320: BWI76_RS08320 - potassium-transporting ATPase subunit KdpA, at 1,761,664 to 1,763,343
_RS08320
BWI76_RS08325: BWI76_RS08325 - potassium-transporting ATPase subunit F, at 1,763,343 to 1,763,432
_RS08325
BWI76_RS08330: BWI76_RS08330 - hypothetical protein, at 1,763,742 to 1,763,948
_RS08330
BWI76_RS08335: BWI76_RS08335 - deoxyribodipyrimidine photo-lyase, at 1,764,151 to 1,765,593
_RS08335
Position (kb)
1761
1762
1763
1764 Strain fitness (log2 ratio)
-1
0
1 at 1760.825 kb on + strand, within BWI76_RS08315 at 1761.348 kb on + strand, within BWI76_RS08315 at 1761.442 kb on - strand at 1761.481 kb on + strand at 1761.591 kb on - strand at 1761.743 kb on + strand at 1761.988 kb on + strand, within BWI76_RS08320 at 1762.134 kb on + strand, within BWI76_RS08320 at 1762.265 kb on + strand, within BWI76_RS08320 at 1762.265 kb on + strand, within BWI76_RS08320 at 1762.753 kb on + strand, within BWI76_RS08320 at 1762.831 kb on + strand, within BWI76_RS08320 at 1763.466 kb on + strand at 1763.674 kb on + strand at 1763.674 kb on + strand at 1763.903 kb on + strand, within BWI76_RS08330 at 1763.968 kb on + strand at 1763.984 kb on - strand at 1764.068 kb on - strand at 1764.085 kb on + strand at 1764.091 kb on + strand at 1764.093 kb on - strand at 1764.244 kb on - strand at 1764.305 kb on - strand, within BWI76_RS08335
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,760,825 + BWI76_RS08315 0.60 +0.9 1,761,348 + BWI76_RS08315 0.86 +0.8 1,761,442 - -0.8 1,761,481 + +0.6 1,761,591 - -0.2 1,761,743 + -0.4 1,761,988 + BWI76_RS08320 0.19 +0.0 1,762,134 + BWI76_RS08320 0.28 +0.2 1,762,265 + BWI76_RS08320 0.36 +0.3 1,762,265 + BWI76_RS08320 0.36 -0.1 1,762,753 + BWI76_RS08320 0.65 +1.1 1,762,831 + BWI76_RS08320 0.69 -0.8 1,763,466 + -1.5 1,763,674 + +0.8 1,763,674 + +0.5 1,763,903 + BWI76_RS08330 0.78 +0.5 1,763,968 + +0.5 1,763,984 - -0.7 1,764,068 - +0.1 1,764,085 + +0.0 1,764,091 + -0.3 1,764,093 - +0.6 1,764,244 - +0.1 1,764,305 - BWI76_RS08335 0.11 -0.4
Or see this region's nucleotide sequence