Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08295 and BWI76_RS08300 are separated by 3 nucleotides BWI76_RS08300 and BWI76_RS08305 overlap by 4 nucleotides BWI76_RS08305 and BWI76_RS08310 are separated by 1 nucleotides BWI76_RS08310 and BWI76_RS08315 are separated by 10 nucleotides
BWI76_RS08295: BWI76_RS08295 - hypothetical protein, at 1,755,222 to 1,755,641
_RS08295
BWI76_RS08300: BWI76_RS08300 - DNA-binding response regulator, at 1,755,645 to 1,756,322
_RS08300
BWI76_RS08305: BWI76_RS08305 - two-component sensor histidine kinase, at 1,756,319 to 1,759,006
_RS08305
BWI76_RS08310: BWI76_RS08310 - potassium-transporting ATPase subunit C, at 1,759,008 to 1,759,583
_RS08310
BWI76_RS08315: BWI76_RS08315 - K+-transporting ATPase subunit B, at 1,759,594 to 1,761,642
_RS08315
Position (kb)
1756
1757
1758
1759
1760 Strain fitness (log2 ratio)
-2
-1
0
1 at 1755.329 kb on - strand, within BWI76_RS08295 at 1755.329 kb on - strand, within BWI76_RS08295 at 1755.393 kb on - strand, within BWI76_RS08295 at 1755.418 kb on - strand, within BWI76_RS08295 at 1755.624 kb on - strand at 1756.151 kb on - strand, within BWI76_RS08300 at 1756.369 kb on + strand at 1756.515 kb on + strand at 1756.749 kb on - strand, within BWI76_RS08305 at 1756.752 kb on + strand, within BWI76_RS08305 at 1757.029 kb on - strand, within BWI76_RS08305 at 1757.109 kb on - strand, within BWI76_RS08305 at 1757.145 kb on + strand, within BWI76_RS08305 at 1757.560 kb on + strand, within BWI76_RS08305 at 1757.560 kb on + strand, within BWI76_RS08305 at 1757.631 kb on + strand, within BWI76_RS08305 at 1757.662 kb on - strand, within BWI76_RS08305 at 1757.892 kb on + strand, within BWI76_RS08305 at 1757.950 kb on + strand, within BWI76_RS08305 at 1757.958 kb on - strand, within BWI76_RS08305 at 1758.017 kb on + strand, within BWI76_RS08305 at 1758.042 kb on - strand, within BWI76_RS08305 at 1758.614 kb on - strand, within BWI76_RS08305 at 1758.665 kb on + strand, within BWI76_RS08305 at 1758.769 kb on + strand at 1759.041 kb on - strand at 1759.200 kb on - strand, within BWI76_RS08310 at 1759.426 kb on - strand, within BWI76_RS08310 at 1759.606 kb on + strand at 1759.952 kb on - strand, within BWI76_RS08315
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,755,329 - BWI76_RS08295 0.25 +0.9 1,755,329 - BWI76_RS08295 0.25 -1.2 1,755,393 - BWI76_RS08295 0.41 -0.5 1,755,418 - BWI76_RS08295 0.47 -2.3 1,755,624 - +0.0 1,756,151 - BWI76_RS08300 0.75 -0.4 1,756,369 + -0.4 1,756,515 + -2.3 1,756,749 - BWI76_RS08305 0.16 +0.6 1,756,752 + BWI76_RS08305 0.16 -0.4 1,757,029 - BWI76_RS08305 0.26 -0.5 1,757,109 - BWI76_RS08305 0.29 +0.5 1,757,145 + BWI76_RS08305 0.31 -0.4 1,757,560 + BWI76_RS08305 0.46 +0.1 1,757,560 + BWI76_RS08305 0.46 -0.8 1,757,631 + BWI76_RS08305 0.49 -0.1 1,757,662 - BWI76_RS08305 0.50 +0.6 1,757,892 + BWI76_RS08305 0.59 +0.6 1,757,950 + BWI76_RS08305 0.61 -0.2 1,757,958 - BWI76_RS08305 0.61 -0.2 1,758,017 + BWI76_RS08305 0.63 -0.4 1,758,042 - BWI76_RS08305 0.64 -0.7 1,758,614 - BWI76_RS08305 0.85 +0.5 1,758,665 + BWI76_RS08305 0.87 +0.8 1,758,769 + -1.1 1,759,041 - +0.8 1,759,200 - BWI76_RS08310 0.33 +0.7 1,759,426 - BWI76_RS08310 0.73 +0.5 1,759,606 + +1.3 1,759,952 - BWI76_RS08315 0.17 +0.1
Or see this region's nucleotide sequence