Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07100 and BWI76_RS07105 are separated by 340 nucleotides BWI76_RS07105 and BWI76_RS07110 are separated by 363 nucleotides BWI76_RS07110 and BWI76_RS07115 are separated by 221 nucleotides BWI76_RS07115 and BWI76_RS07120 are separated by 115 nucleotides
BWI76_RS07100: BWI76_RS07100 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 1,491,787 to 1,492,653
_RS07100
BWI76_RS07105: BWI76_RS07105 - tRNA-Arg, at 1,492,994 to 1,493,070
_RS07105
BWI76_RS07110: BWI76_RS07110 - DNA-binding protein, at 1,493,434 to 1,493,891
_RS07110
BWI76_RS07115: BWI76_RS07115 - hypothetical protein, at 1,494,113 to 1,494,409
_RS07115
BWI76_RS07120: BWI76_RS07120 - LacI family transcriptional regulator, at 1,494,525 to 1,496,699
_RS07120
Position (kb) 1493
1494 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1492.770 kb on - strand at 1492.905 kb on - strand at 1492.951 kb on + strand at 1493.095 kb on - strand at 1493.099 kb on + strand at 1493.145 kb on - strand at 1493.152 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.159 kb on - strand at 1493.162 kb on + strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.167 kb on + strand at 1493.167 kb on + strand at 1493.167 kb on + strand at 1493.167 kb on + strand at 1493.172 kb on + strand at 1493.175 kb on - strand at 1493.175 kb on - strand at 1493.175 kb on - strand at 1493.175 kb on - strand at 1493.177 kb on + strand at 1493.177 kb on + strand at 1493.180 kb on + strand at 1493.185 kb on - strand at 1493.185 kb on - strand at 1493.187 kb on - strand at 1493.187 kb on - strand at 1493.187 kb on - strand at 1493.188 kb on - strand at 1493.194 kb on - strand at 1493.194 kb on - strand at 1493.215 kb on + strand at 1493.216 kb on - strand at 1493.223 kb on - strand at 1493.225 kb on + strand at 1493.230 kb on - strand at 1493.231 kb on + strand at 1493.233 kb on - strand at 1493.239 kb on - strand at 1493.240 kb on - strand at 1493.295 kb on - strand at 1493.314 kb on + strand at 1493.319 kb on + strand at 1493.321 kb on + strand at 1493.322 kb on - strand at 1493.322 kb on - strand at 1493.327 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.338 kb on + strand at 1493.436 kb on + strand at 1493.454 kb on - strand at 1493.516 kb on + strand, within BWI76_RS07110 at 1493.576 kb on - strand, within BWI76_RS07110 at 1493.659 kb on - strand, within BWI76_RS07110 at 1493.668 kb on - strand, within BWI76_RS07110 at 1493.669 kb on - strand, within BWI76_RS07110 at 1493.697 kb on + strand, within BWI76_RS07110 at 1493.697 kb on + strand, within BWI76_RS07110 at 1493.697 kb on + strand, within BWI76_RS07110 at 1493.697 kb on + strand, within BWI76_RS07110 at 1493.697 kb on + strand, within BWI76_RS07110 at 1493.697 kb on + strand, within BWI76_RS07110 at 1493.733 kb on + strand, within BWI76_RS07110 at 1493.761 kb on + strand, within BWI76_RS07110 at 1493.939 kb on - strand at 1493.985 kb on - strand at 1493.991 kb on - strand at 1494.078 kb on + strand at 1494.109 kb on + strand at 1494.132 kb on + strand at 1494.138 kb on - strand at 1494.154 kb on - strand, within BWI76_RS07115 at 1494.154 kb on - strand, within BWI76_RS07115 at 1494.169 kb on - strand, within BWI76_RS07115 at 1494.169 kb on - strand, within BWI76_RS07115 at 1494.249 kb on + strand, within BWI76_RS07115 at 1494.269 kb on - strand, within BWI76_RS07115 at 1494.292 kb on - strand, within BWI76_RS07115 at 1494.302 kb on + strand, within BWI76_RS07115 at 1494.314 kb on + strand, within BWI76_RS07115 at 1494.314 kb on + strand at 1494.319 kb on + strand, within BWI76_RS07115 at 1494.319 kb on + strand, within BWI76_RS07115 at 1494.319 kb on + strand, within BWI76_RS07115 at 1494.319 kb on + strand, within BWI76_RS07115 at 1494.319 kb on + strand, within BWI76_RS07115 at 1494.327 kb on - strand, within BWI76_RS07115 at 1494.327 kb on - strand at 1494.327 kb on - strand, within BWI76_RS07115 at 1494.333 kb on + strand, within BWI76_RS07115 at 1494.366 kb on + strand, within BWI76_RS07115 at 1494.377 kb on + strand, within BWI76_RS07115 at 1494.395 kb on - strand at 1494.434 kb on + strand at 1494.434 kb on + strand at 1494.442 kb on - strand at 1494.442 kb on - strand at 1494.457 kb on + strand at 1494.465 kb on - strand at 1494.484 kb on - strand at 1494.505 kb on - strand at 1494.558 kb on + strand at 1494.571 kb on + strand at 1494.579 kb on - strand at 1494.579 kb on - strand at 1494.644 kb on + strand at 1494.652 kb on + strand at 1494.652 kb on + strand at 1494.652 kb on + strand at 1494.652 kb on + strand at 1494.652 kb on + strand at 1494.652 kb on - strand at 1494.660 kb on - strand at 1494.660 kb on - strand at 1494.660 kb on - strand at 1494.660 kb on - strand at 1494.660 kb on - strand at 1494.660 kb on - strand at 1494.660 kb on - strand at 1494.660 kb on - strand at 1494.677 kb on + strand at 1494.677 kb on + strand at 1494.677 kb on + strand at 1494.677 kb on + strand at 1494.677 kb on + strand at 1494.685 kb on - strand at 1494.685 kb on - strand at 1494.688 kb on + strand at 1494.728 kb on + strand at 1494.729 kb on + strand at 1494.736 kb on - strand at 1494.740 kb on + strand at 1494.775 kb on + strand, within BWI76_RS07120 at 1494.780 kb on + strand, within BWI76_RS07120 at 1494.783 kb on - strand, within BWI76_RS07120 at 1494.783 kb on - strand, within BWI76_RS07120 at 1494.785 kb on + strand, within BWI76_RS07120
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,492,770 - -0.2 1,492,905 - -0.8 1,492,951 + -0.2 1,493,095 - -1.1 1,493,099 + +0.2 1,493,145 - +1.3 1,493,152 + +1.9 1,493,157 + -1.1 1,493,157 + +0.9 1,493,157 + -0.3 1,493,157 + -2.3 1,493,157 + -0.7 1,493,157 + +1.7 1,493,157 + -0.4 1,493,159 - -0.3 1,493,162 + +0.5 1,493,165 - -0.1 1,493,165 - -1.5 1,493,165 - -1.1 1,493,165 - +0.9 1,493,165 - +0.2 1,493,167 + -2.0 1,493,167 + -0.1 1,493,167 + -0.8 1,493,167 + -0.1 1,493,172 + +1.5 1,493,175 - +1.9 1,493,175 - -0.7 1,493,175 - -0.0 1,493,175 - +0.9 1,493,177 + +1.1 1,493,177 + -1.4 1,493,180 + +1.7 1,493,185 - -0.1 1,493,185 - -1.5 1,493,187 - +0.4 1,493,187 - -0.8 1,493,187 - -0.1 1,493,188 - -0.1 1,493,194 - -0.7 1,493,194 - -1.9 1,493,215 + -0.0 1,493,216 - +0.9 1,493,223 - -2.6 1,493,225 + -1.4 1,493,230 - -0.1 1,493,231 + +0.9 1,493,233 - +1.0 1,493,239 - -0.7 1,493,240 - -2.2 1,493,295 - -0.2 1,493,314 + -1.7 1,493,319 + -0.7 1,493,321 + -1.4 1,493,322 - -1.1 1,493,322 - +0.9 1,493,327 + -1.1 1,493,328 + -0.7 1,493,328 + +0.9 1,493,328 + +0.7 1,493,328 + +0.8 1,493,328 + -1.4 1,493,328 + -1.9 1,493,328 + -0.1 1,493,329 - +0.3 1,493,329 - -1.7 1,493,329 - -0.7 1,493,329 - -1.8 1,493,329 - -1.1 1,493,336 - +0.6 1,493,336 - -0.2 1,493,336 - +0.9 1,493,336 - -0.1 1,493,336 - +0.6 1,493,336 - -0.6 1,493,336 - -0.3 1,493,338 + +0.9 1,493,436 + +0.3 1,493,454 - -0.7 1,493,516 + BWI76_RS07110 0.18 -1.4 1,493,576 - BWI76_RS07110 0.31 -0.1 1,493,659 - BWI76_RS07110 0.49 -0.1 1,493,668 - BWI76_RS07110 0.51 -1.9 1,493,669 - BWI76_RS07110 0.51 -0.7 1,493,697 + BWI76_RS07110 0.57 -0.8 1,493,697 + BWI76_RS07110 0.57 +0.9 1,493,697 + BWI76_RS07110 0.57 -1.4 1,493,697 + BWI76_RS07110 0.57 -0.1 1,493,697 + BWI76_RS07110 0.57 -0.4 1,493,697 + BWI76_RS07110 0.57 -2.6 1,493,733 + BWI76_RS07110 0.65 -1.2 1,493,761 + BWI76_RS07110 0.71 +1.1 1,493,939 - -1.7 1,493,985 - -0.7 1,493,991 - +0.9 1,494,078 + -1.9 1,494,109 + +1.9 1,494,132 + +0.9 1,494,138 - -0.7 1,494,154 - BWI76_RS07115 0.14 +0.4 1,494,154 - BWI76_RS07115 0.14 -2.9 1,494,169 - BWI76_RS07115 0.19 -0.3 1,494,169 - BWI76_RS07115 0.19 -1.1 1,494,249 + BWI76_RS07115 0.46 -1.1 1,494,269 - BWI76_RS07115 0.53 +0.9 1,494,292 - BWI76_RS07115 0.60 -0.7 1,494,302 + BWI76_RS07115 0.64 +0.1 1,494,314 + BWI76_RS07115 0.68 +0.9 1,494,314 + +0.9 1,494,319 + BWI76_RS07115 0.69 -2.1 1,494,319 + BWI76_RS07115 0.69 +0.6 1,494,319 + BWI76_RS07115 0.69 -0.3 1,494,319 + BWI76_RS07115 0.69 +0.4 1,494,319 + BWI76_RS07115 0.69 +0.2 1,494,327 - BWI76_RS07115 0.72 +0.9 1,494,327 - -0.7 1,494,327 - BWI76_RS07115 0.72 -1.7 1,494,333 + BWI76_RS07115 0.74 -1.1 1,494,366 + BWI76_RS07115 0.85 -0.8 1,494,377 + BWI76_RS07115 0.89 -0.7 1,494,395 - -1.7 1,494,434 + +0.9 1,494,434 + +0.2 1,494,442 - -0.1 1,494,442 - -0.8 1,494,457 + +0.9 1,494,465 - +0.0 1,494,484 - -1.9 1,494,505 - -0.1 1,494,558 + -0.7 1,494,571 + -0.7 1,494,579 - -1.6 1,494,579 - -0.6 1,494,644 + -0.4 1,494,652 + -0.8 1,494,652 + -2.0 1,494,652 + -1.9 1,494,652 + -0.1 1,494,652 + +1.2 1,494,652 - -0.4 1,494,660 - -0.1 1,494,660 - -1.9 1,494,660 - -2.4 1,494,660 - -0.3 1,494,660 - +0.2 1,494,660 - +0.9 1,494,660 - -0.1 1,494,660 - -2.1 1,494,677 + -0.7 1,494,677 + -0.2 1,494,677 + -0.1 1,494,677 + +0.1 1,494,677 + -0.1 1,494,685 - -1.1 1,494,685 - -0.1 1,494,688 + +0.2 1,494,728 + +0.9 1,494,729 + -1.1 1,494,736 - -0.8 1,494,740 + -0.4 1,494,775 + BWI76_RS07120 0.11 -1.1 1,494,780 + BWI76_RS07120 0.12 +0.7 1,494,783 - BWI76_RS07120 0.12 -1.4 1,494,783 - BWI76_RS07120 0.12 -0.8 1,494,785 + BWI76_RS07120 0.12 -0.3
Or see this region's nucleotide sequence