Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06850 and BWI76_RS06855 are separated by 42 nucleotides BWI76_RS06855 and BWI76_RS06860 are separated by 233 nucleotides
BWI76_RS06850: BWI76_RS06850 - inosine/guanosine kinase, at 1,436,947 to 1,438,251
_RS06850
BWI76_RS06855: BWI76_RS06855 - Kef family K(+) transporter, at 1,438,294 to 1,439,970
_RS06855
BWI76_RS06860: BWI76_RS06860 - MFS transporter, at 1,440,204 to 1,441,424
_RS06860
Position (kb) 1438
1439
1440 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1437.352 kb on - strand, within BWI76_RS06850 at 1437.401 kb on + strand, within BWI76_RS06850 at 1437.625 kb on - strand, within BWI76_RS06850 at 1437.837 kb on + strand, within BWI76_RS06850 at 1438.390 kb on + strand at 1438.539 kb on - strand, within BWI76_RS06855 at 1438.581 kb on - strand, within BWI76_RS06855 at 1438.659 kb on - strand, within BWI76_RS06855 at 1438.767 kb on + strand, within BWI76_RS06855 at 1438.815 kb on + strand, within BWI76_RS06855 at 1438.963 kb on + strand, within BWI76_RS06855 at 1438.963 kb on + strand, within BWI76_RS06855 at 1438.971 kb on - strand, within BWI76_RS06855 at 1438.971 kb on - strand, within BWI76_RS06855 at 1438.971 kb on - strand, within BWI76_RS06855 at 1439.116 kb on + strand, within BWI76_RS06855 at 1439.160 kb on - strand, within BWI76_RS06855 at 1439.238 kb on + strand, within BWI76_RS06855 at 1439.648 kb on + strand, within BWI76_RS06855 at 1439.662 kb on + strand, within BWI76_RS06855 at 1439.918 kb on - strand at 1439.985 kb on + strand at 1440.419 kb on - strand, within BWI76_RS06860 at 1440.501 kb on - strand, within BWI76_RS06860 at 1440.769 kb on + strand, within BWI76_RS06860 at 1440.848 kb on + strand, within BWI76_RS06860
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,437,352 - BWI76_RS06850 0.31 +0.5 1,437,401 + BWI76_RS06850 0.35 -2.1 1,437,625 - BWI76_RS06850 0.52 -0.1 1,437,837 + BWI76_RS06850 0.68 +0.9 1,438,390 + +0.9 1,438,539 - BWI76_RS06855 0.15 -0.2 1,438,581 - BWI76_RS06855 0.17 +0.1 1,438,659 - BWI76_RS06855 0.22 -2.1 1,438,767 + BWI76_RS06855 0.28 +0.2 1,438,815 + BWI76_RS06855 0.31 +0.3 1,438,963 + BWI76_RS06855 0.40 +0.4 1,438,963 + BWI76_RS06855 0.40 +0.6 1,438,971 - BWI76_RS06855 0.40 +0.4 1,438,971 - BWI76_RS06855 0.40 -0.5 1,438,971 - BWI76_RS06855 0.40 -0.7 1,439,116 + BWI76_RS06855 0.49 +0.9 1,439,160 - BWI76_RS06855 0.52 -4.1 1,439,238 + BWI76_RS06855 0.56 -0.2 1,439,648 + BWI76_RS06855 0.81 +0.1 1,439,662 + BWI76_RS06855 0.82 -0.1 1,439,918 - +0.1 1,439,985 + +0.3 1,440,419 - BWI76_RS06860 0.18 +0.6 1,440,501 - BWI76_RS06860 0.24 -0.3 1,440,769 + BWI76_RS06860 0.46 +0.9 1,440,848 + BWI76_RS06860 0.53 -1.5
Or see this region's nucleotide sequence