Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04485 and BWI76_RS04490 are separated by 166 nucleotides BWI76_RS04490 and BWI76_RS04495 overlap by 8 nucleotides BWI76_RS04495 and BWI76_RS04500 are separated by 84 nucleotides BWI76_RS04500 and BWI76_RS04505 overlap by 1 nucleotides BWI76_RS04505 and BWI76_RS04510 are separated by 40 nucleotides
BWI76_RS04485: BWI76_RS04485 - hypothetical protein, at 928,559 to 930,115
_RS04485
BWI76_RS04490: BWI76_RS04490 - glutathione-regulated potassium-efflux system ancillary protein KefF, at 930,282 to 930,815
_RS04490
BWI76_RS04495: BWI76_RS04495 - glutathione-regulated potassium-efflux system protein KefC, at 930,808 to 932,673
_RS04495
BWI76_RS04500: BWI76_RS04500 - dihydrofolate reductase, at 932,758 to 932,871
_RS04500
BWI76_RS04505: BWI76_RS04505 - dihydrofolate reductase, at 932,871 to 933,350
_RS04505
BWI76_RS04510: BWI76_RS04510 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 933,391 to 934,239
_RS04510
Position (kb) 930
931
932
933 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 929.858 kb on - strand, within BWI76_RS04485 at 929.867 kb on - strand, within BWI76_RS04485 at 929.872 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.904 kb on + strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.912 kb on - strand, within BWI76_RS04485 at 929.958 kb on + strand, within BWI76_RS04485 at 929.958 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.959 kb on + strand, within BWI76_RS04485 at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 929.967 kb on - strand at 930.037 kb on - strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.039 kb on + strand at 930.041 kb on + strand at 930.047 kb on - strand at 930.074 kb on + strand at 930.074 kb on + strand at 930.082 kb on - strand at 930.093 kb on - strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.109 kb on + strand at 930.114 kb on + strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.117 kb on - strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.119 kb on + strand at 930.127 kb on - strand at 930.135 kb on - strand at 930.139 kb on + strand at 930.147 kb on - strand at 930.147 kb on - strand at 930.159 kb on + strand at 930.166 kb on + strand at 930.166 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on + strand at 930.174 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.182 kb on - strand at 930.184 kb on + strand at 930.184 kb on + strand at 930.184 kb on + strand at 930.185 kb on + strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.192 kb on - strand at 930.215 kb on - strand at 930.243 kb on + strand at 930.244 kb on - strand at 930.245 kb on - strand at 930.251 kb on - strand at 930.253 kb on - strand at 930.259 kb on - strand at 930.263 kb on + strand at 930.264 kb on - strand at 930.264 kb on - strand at 930.270 kb on - strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.305 kb on + strand at 930.313 kb on - strand at 930.313 kb on - strand at 930.313 kb on - strand at 930.334 kb on + strand at 930.340 kb on + strand, within BWI76_RS04490 at 930.340 kb on + strand, within BWI76_RS04490 at 930.350 kb on + strand, within BWI76_RS04490 at 930.358 kb on - strand, within BWI76_RS04490 at 930.367 kb on + strand, within BWI76_RS04490 at 930.367 kb on + strand, within BWI76_RS04490 at 930.369 kb on + strand, within BWI76_RS04490 at 930.380 kb on - strand, within BWI76_RS04490 at 930.421 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.435 kb on + strand, within BWI76_RS04490 at 930.443 kb on - strand, within BWI76_RS04490 at 930.445 kb on + strand, within BWI76_RS04490 at 930.485 kb on - strand, within BWI76_RS04490 at 930.495 kb on - strand, within BWI76_RS04490 at 930.523 kb on - strand, within BWI76_RS04490 at 930.528 kb on + strand, within BWI76_RS04490 at 930.533 kb on + strand, within BWI76_RS04490 at 930.680 kb on + strand, within BWI76_RS04490 at 930.815 kb on + strand at 930.815 kb on + strand at 930.881 kb on + strand at 930.889 kb on - strand at 930.997 kb on + strand, within BWI76_RS04495 at 931.027 kb on - strand, within BWI76_RS04495 at 931.042 kb on - strand, within BWI76_RS04495 at 931.116 kb on + strand, within BWI76_RS04495 at 931.169 kb on + strand, within BWI76_RS04495 at 931.179 kb on + strand, within BWI76_RS04495 at 931.187 kb on - strand, within BWI76_RS04495 at 931.250 kb on + strand, within BWI76_RS04495 at 931.795 kb on - strand, within BWI76_RS04495 at 931.866 kb on - strand, within BWI76_RS04495 at 932.119 kb on - strand, within BWI76_RS04495 at 932.162 kb on + strand, within BWI76_RS04495 at 932.315 kb on + strand, within BWI76_RS04495 at 932.344 kb on - strand, within BWI76_RS04495 at 932.486 kb on - strand, within BWI76_RS04495 at 932.600 kb on + strand at 932.655 kb on + strand at 932.674 kb on + strand at 932.750 kb on + strand at 933.478 kb on + strand, within BWI76_RS04510 at 933.624 kb on + strand, within BWI76_RS04510
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 929,858 - BWI76_RS04485 0.83 +1.2 929,867 - BWI76_RS04485 0.84 +1.7 929,872 + BWI76_RS04485 0.84 -1.0 929,904 + BWI76_RS04485 0.86 -1.3 929,904 + BWI76_RS04485 0.86 -1.3 929,904 + BWI76_RS04485 0.86 +0.4 929,904 + BWI76_RS04485 0.86 -1.0 929,904 + BWI76_RS04485 0.86 +0.5 929,904 + BWI76_RS04485 0.86 -0.8 929,904 + BWI76_RS04485 0.86 -1.3 929,912 - BWI76_RS04485 0.87 -0.6 929,912 - BWI76_RS04485 0.87 -0.2 929,912 - BWI76_RS04485 0.87 -0.8 929,912 - BWI76_RS04485 0.87 -1.8 929,912 - BWI76_RS04485 0.87 -0.4 929,912 - BWI76_RS04485 0.87 +0.3 929,958 + BWI76_RS04485 0.90 +1.2 929,958 + BWI76_RS04485 0.90 +1.0 929,959 + BWI76_RS04485 0.90 +0.0 929,959 + BWI76_RS04485 0.90 -1.8 929,959 + BWI76_RS04485 0.90 -1.3 929,959 + BWI76_RS04485 0.90 +0.4 929,959 + BWI76_RS04485 0.90 -0.7 929,967 - -1.0 929,967 - -1.3 929,967 - +0.0 929,967 - +1.0 929,967 - +0.0 929,967 - +0.0 929,967 - +1.0 930,037 - -0.2 930,039 + -0.7 930,039 + +0.2 930,039 + -0.6 930,039 + +0.4 930,039 + -0.6 930,039 + +0.0 930,039 + +1.6 930,039 + +1.2 930,039 + -0.1 930,041 + -0.2 930,047 - +1.0 930,074 + +1.1 930,074 + +0.1 930,082 - -2.0 930,093 - -1.2 930,109 + +0.5 930,109 + +1.1 930,109 + +0.7 930,109 + +1.0 930,109 + +1.0 930,109 + -1.3 930,109 + -2.3 930,109 + -0.1 930,114 + +1.1 930,117 - -2.3 930,117 - -0.7 930,117 - +1.6 930,117 - -0.2 930,119 + -1.0 930,119 + -0.3 930,119 + +0.7 930,119 + -0.2 930,119 + +0.6 930,127 - +1.0 930,135 - +0.0 930,139 + -0.1 930,147 - -0.1 930,147 - +2.0 930,159 + +0.4 930,166 + +0.1 930,166 + +2.1 930,174 + -0.4 930,174 + -1.6 930,174 + -0.5 930,174 + -2.6 930,174 - +1.5 930,182 - +2.0 930,182 - +0.7 930,182 - -1.3 930,182 - +0.9 930,182 - +0.4 930,182 - +0.2 930,182 - -0.3 930,182 - -0.3 930,182 - +0.6 930,182 - +0.5 930,182 - +0.3 930,182 - +0.1 930,182 - -1.3 930,182 - +0.9 930,184 + +0.4 930,184 + +0.2 930,184 + +0.0 930,185 + -2.1 930,192 - -0.2 930,192 - +0.1 930,192 - -0.7 930,192 - -0.3 930,192 - +0.0 930,192 - +0.5 930,215 - +1.0 930,243 + -1.6 930,244 - +2.6 930,245 - -0.1 930,251 - -0.2 930,253 - +0.2 930,259 - +1.4 930,263 + +0.1 930,264 - +2.5 930,264 - -1.0 930,270 - -0.5 930,305 + -0.9 930,305 + -1.3 930,305 + +1.9 930,305 + -0.7 930,305 + +1.6 930,313 - -0.4 930,313 - -0.2 930,313 - -0.2 930,334 + +0.3 930,340 + BWI76_RS04490 0.11 +0.1 930,340 + BWI76_RS04490 0.11 +0.3 930,350 + BWI76_RS04490 0.13 +0.2 930,358 - BWI76_RS04490 0.14 +0.5 930,367 + BWI76_RS04490 0.16 +0.0 930,367 + BWI76_RS04490 0.16 +0.3 930,369 + BWI76_RS04490 0.16 +0.1 930,380 - BWI76_RS04490 0.18 +1.0 930,421 + BWI76_RS04490 0.26 -0.1 930,435 + BWI76_RS04490 0.29 +0.2 930,435 + BWI76_RS04490 0.29 -0.7 930,435 + BWI76_RS04490 0.29 -0.7 930,443 - BWI76_RS04490 0.30 -0.6 930,445 + BWI76_RS04490 0.31 +0.7 930,485 - BWI76_RS04490 0.38 +0.2 930,495 - BWI76_RS04490 0.40 +3.6 930,523 - BWI76_RS04490 0.45 +0.4 930,528 + BWI76_RS04490 0.46 +1.3 930,533 + BWI76_RS04490 0.47 -0.1 930,680 + BWI76_RS04490 0.75 +1.0 930,815 + -0.6 930,815 + -0.8 930,881 + +0.5 930,889 - +0.1 930,997 + BWI76_RS04495 0.10 +0.6 931,027 - BWI76_RS04495 0.12 +0.5 931,042 - BWI76_RS04495 0.13 +1.6 931,116 + BWI76_RS04495 0.17 +0.5 931,169 + BWI76_RS04495 0.19 +1.1 931,179 + BWI76_RS04495 0.20 +0.9 931,187 - BWI76_RS04495 0.20 -0.6 931,250 + BWI76_RS04495 0.24 +0.3 931,795 - BWI76_RS04495 0.53 -1.8 931,866 - BWI76_RS04495 0.57 +0.6 932,119 - BWI76_RS04495 0.70 -0.0 932,162 + BWI76_RS04495 0.73 +1.4 932,315 + BWI76_RS04495 0.81 +0.7 932,344 - BWI76_RS04495 0.82 +0.2 932,486 - BWI76_RS04495 0.90 -1.7 932,600 + +0.0 932,655 + +0.5 932,674 + -0.3 932,750 + +0.5 933,478 + BWI76_RS04510 0.10 +1.0 933,624 + BWI76_RS04510 0.27 -0.6
Or see this region's nucleotide sequence