Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04090 and BWI76_RS04095 are separated by 14 nucleotides BWI76_RS04095 and BWI76_RS04100 are separated by 91 nucleotides BWI76_RS04100 and BWI76_RS04105 are separated by 217 nucleotides BWI76_RS04105 and BWI76_RS04110 are separated by 123 nucleotides
BWI76_RS04090: BWI76_RS04090 - ribosomal-protein-alanine N-acetyltransferase RimI, at 841,827 to 842,273
_RS04090
BWI76_RS04095: BWI76_RS04095 - dUMP phosphatase, at 842,288 to 842,965
_RS04095
BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646
_RS04100
BWI76_RS04105: BWI76_RS04105 - molecular chaperone OsmY, at 844,864 to 845,475
_RS04105
BWI76_RS04110: BWI76_RS04110 - DUF1328 domain-containing protein, at 845,599 to 845,760
_RS04110
Position (kb)
843
844
845 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 842.066 kb on + strand, within BWI76_RS04090 at 842.076 kb on - strand, within BWI76_RS04090 at 842.128 kb on + strand, within BWI76_RS04090 at 842.143 kb on + strand, within BWI76_RS04090 at 842.730 kb on - strand, within BWI76_RS04095 at 842.876 kb on - strand, within BWI76_RS04095 at 843.799 kb on - strand, within BWI76_RS04100 at 843.868 kb on + strand, within BWI76_RS04100 at 844.010 kb on - strand, within BWI76_RS04100 at 844.121 kb on + strand, within BWI76_RS04100 at 844.189 kb on + strand, within BWI76_RS04100 at 844.196 kb on - strand, within BWI76_RS04100 at 844.247 kb on + strand, within BWI76_RS04100 at 844.482 kb on - strand, within BWI76_RS04100 at 844.899 kb on - strand at 844.923 kb on - strand at 844.951 kb on + strand, within BWI76_RS04105 at 844.951 kb on + strand, within BWI76_RS04105 at 844.951 kb on + strand, within BWI76_RS04105 at 844.959 kb on - strand, within BWI76_RS04105 at 845.063 kb on - strand, within BWI76_RS04105 at 845.063 kb on - strand, within BWI76_RS04105 at 845.097 kb on + strand, within BWI76_RS04105 at 845.133 kb on - strand, within BWI76_RS04105 at 845.141 kb on + strand, within BWI76_RS04105 at 845.259 kb on + strand, within BWI76_RS04105 at 845.368 kb on - strand, within BWI76_RS04105 at 845.385 kb on - strand, within BWI76_RS04105 at 845.409 kb on + strand, within BWI76_RS04105 at 845.436 kb on - strand at 845.527 kb on + strand at 845.527 kb on + strand at 845.527 kb on + strand at 845.527 kb on - strand at 845.535 kb on - strand at 845.535 kb on - strand at 845.540 kb on - strand at 845.582 kb on + strand at 845.589 kb on + strand at 845.590 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 842,066 + BWI76_RS04090 0.53 +0.7 842,076 - BWI76_RS04090 0.56 -1.3 842,128 + BWI76_RS04090 0.67 -2.3 842,143 + BWI76_RS04090 0.71 +0.7 842,730 - BWI76_RS04095 0.65 +1.0 842,876 - BWI76_RS04095 0.87 +0.5 843,799 - BWI76_RS04100 0.47 +1.0 843,868 + BWI76_RS04100 0.51 -1.0 844,010 - BWI76_RS04100 0.60 -0.9 844,121 + BWI76_RS04100 0.67 +0.0 844,189 + BWI76_RS04100 0.71 +0.0 844,196 - BWI76_RS04100 0.72 -2.4 844,247 + BWI76_RS04100 0.75 -1.8 844,482 - BWI76_RS04100 0.90 -0.7 844,899 - -0.5 844,923 - +0.1 844,951 + BWI76_RS04105 0.14 -0.4 844,951 + BWI76_RS04105 0.14 +0.0 844,951 + BWI76_RS04105 0.14 +1.3 844,959 - BWI76_RS04105 0.16 -0.4 845,063 - BWI76_RS04105 0.33 -0.5 845,063 - BWI76_RS04105 0.33 +0.6 845,097 + BWI76_RS04105 0.38 +0.4 845,133 - BWI76_RS04105 0.44 +0.4 845,141 + BWI76_RS04105 0.45 +0.4 845,259 + BWI76_RS04105 0.65 +0.7 845,368 - BWI76_RS04105 0.82 +2.3 845,385 - BWI76_RS04105 0.85 -0.6 845,409 + BWI76_RS04105 0.89 -0.7 845,436 - +0.0 845,527 + -1.1 845,527 + +0.5 845,527 + -0.2 845,527 - +0.0 845,535 - -0.9 845,535 - -1.6 845,540 - +0.7 845,582 + -0.3 845,589 + +1.0 845,590 - +0.6
Or see this region's nucleotide sequence