Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02770 and BWI76_RS02775 are separated by 209 nucleotides BWI76_RS02775 and BWI76_RS02780 overlap by 4 nucleotides
BWI76_RS02770: BWI76_RS02770 - peptide-methionine (S)-S-oxide reductase, at 562,366 to 563,004
_RS02770
BWI76_RS02775: BWI76_RS02775 - outer membrane protein assembly factor, at 563,214 to 564,947
_RS02775
BWI76_RS02780: BWI76_RS02780 - translocation and assembly module TamB, at 564,944 to 568,720
_RS02780
Position (kb) 563
564
565 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 562.284 kb on + strand at 562.284 kb on + strand at 562.292 kb on - strand at 562.292 kb on - strand at 562.539 kb on + strand, within BWI76_RS02770 at 562.572 kb on - strand, within BWI76_RS02770 at 562.572 kb on - strand, within BWI76_RS02770 at 562.636 kb on - strand, within BWI76_RS02770 at 562.636 kb on - strand, within BWI76_RS02770 at 562.672 kb on - strand, within BWI76_RS02770 at 562.685 kb on - strand, within BWI76_RS02770 at 562.741 kb on + strand, within BWI76_RS02770 at 562.741 kb on + strand, within BWI76_RS02770 at 562.747 kb on - strand, within BWI76_RS02770 at 562.799 kb on + strand, within BWI76_RS02770 at 562.833 kb on - strand, within BWI76_RS02770 at 562.850 kb on - strand, within BWI76_RS02770 at 562.916 kb on + strand, within BWI76_RS02770 at 562.926 kb on - strand, within BWI76_RS02770 at 562.998 kb on + strand at 563.012 kb on - strand at 563.038 kb on + strand at 563.132 kb on + strand at 563.156 kb on + strand at 563.186 kb on - strand at 563.579 kb on - strand, within BWI76_RS02775 at 563.782 kb on + strand, within BWI76_RS02775 at 563.797 kb on + strand, within BWI76_RS02775 at 564.194 kb on - strand, within BWI76_RS02775 at 564.211 kb on + strand, within BWI76_RS02775 at 564.344 kb on - strand, within BWI76_RS02775 at 564.458 kb on + strand, within BWI76_RS02775 at 564.466 kb on + strand, within BWI76_RS02775 at 564.596 kb on + strand, within BWI76_RS02775 at 564.965 kb on + strand at 565.182 kb on + strand at 565.321 kb on + strand at 565.348 kb on - strand, within BWI76_RS02780 at 565.358 kb on - strand, within BWI76_RS02780 at 565.546 kb on + strand, within BWI76_RS02780 at 565.580 kb on - strand, within BWI76_RS02780 at 565.698 kb on + strand, within BWI76_RS02780 at 565.706 kb on - strand, within BWI76_RS02780 at 565.706 kb on - strand, within BWI76_RS02780 at 565.769 kb on + strand, within BWI76_RS02780 at 565.774 kb on + strand, within BWI76_RS02780
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 562,284 + -0.4 562,284 + -0.4 562,292 - +0.6 562,292 - +0.3 562,539 + BWI76_RS02770 0.27 +0.6 562,572 - BWI76_RS02770 0.32 +0.4 562,572 - BWI76_RS02770 0.32 -0.2 562,636 - BWI76_RS02770 0.42 -0.5 562,636 - BWI76_RS02770 0.42 -1.8 562,672 - BWI76_RS02770 0.48 +0.8 562,685 - BWI76_RS02770 0.50 -0.1 562,741 + BWI76_RS02770 0.59 -0.3 562,741 + BWI76_RS02770 0.59 -0.2 562,747 - BWI76_RS02770 0.60 +0.6 562,799 + BWI76_RS02770 0.68 -1.8 562,833 - BWI76_RS02770 0.73 +1.0 562,850 - BWI76_RS02770 0.76 -0.2 562,916 + BWI76_RS02770 0.86 +0.3 562,926 - BWI76_RS02770 0.88 +0.0 562,998 + +0.2 563,012 - -0.0 563,038 + -0.4 563,132 + +0.2 563,156 + +0.1 563,186 - +1.5 563,579 - BWI76_RS02775 0.21 -1.6 563,782 + BWI76_RS02775 0.33 -2.5 563,797 + BWI76_RS02775 0.34 -2.1 564,194 - BWI76_RS02775 0.57 -0.8 564,211 + BWI76_RS02775 0.57 -0.8 564,344 - BWI76_RS02775 0.65 -3.6 564,458 + BWI76_RS02775 0.72 -2.5 564,466 + BWI76_RS02775 0.72 -1.1 564,596 + BWI76_RS02775 0.80 -1.0 564,965 + -1.1 565,182 + -2.5 565,321 + -3.8 565,348 - BWI76_RS02780 0.11 -2.3 565,358 - BWI76_RS02780 0.11 -2.3 565,546 + BWI76_RS02780 0.16 -3.8 565,580 - BWI76_RS02780 0.17 -3.0 565,698 + BWI76_RS02780 0.20 -0.8 565,706 - BWI76_RS02780 0.20 -2.1 565,706 - BWI76_RS02780 0.20 -1.8 565,769 + BWI76_RS02780 0.22 -2.0 565,774 + BWI76_RS02780 0.22 -3.0
Or see this region's nucleotide sequence