Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02725

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02720 and BWI76_RS02725 are separated by 84 nucleotidesBWI76_RS02725 and BWI76_RS02730 are separated by 52 nucleotides BWI76_RS02720: BWI76_RS02720 - NAD(P)-dependent oxidoreductase, at 551,086 to 551,934 _RS02720 BWI76_RS02725: BWI76_RS02725 - HxlR family transcriptional regulator, at 552,019 to 552,402 _RS02725 BWI76_RS02730: BWI76_RS02730 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 552,455 to 554,398 _RS02730 Position (kb) 552 553Strain fitness (log2 ratio) -1 0 1at 551.229 kb on + strand, within BWI76_RS02720at 551.237 kb on - strand, within BWI76_RS02720at 551.237 kb on - strand, within BWI76_RS02720at 551.318 kb on - strand, within BWI76_RS02720at 551.341 kb on - strand, within BWI76_RS02720at 551.543 kb on - strand, within BWI76_RS02720at 551.604 kb on - strand, within BWI76_RS02720at 551.666 kb on + strand, within BWI76_RS02720at 551.854 kb on - strandat 551.901 kb on - strandat 551.937 kb on + strandat 552.264 kb on - strand, within BWI76_RS02725at 552.409 kb on + strandat 552.437 kb on - strandat 552.688 kb on + strand, within BWI76_RS02730at 553.210 kb on + strand, within BWI76_RS02730at 553.225 kb on + strand, within BWI76_RS02730

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
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551,229 + BWI76_RS02720 0.17 +0.0
551,237 - BWI76_RS02720 0.18 -0.2
551,237 - BWI76_RS02720 0.18 +0.3
551,318 - BWI76_RS02720 0.27 +1.1
551,341 - BWI76_RS02720 0.30 +0.2
551,543 - BWI76_RS02720 0.54 +0.9
551,604 - BWI76_RS02720 0.61 +0.4
551,666 + BWI76_RS02720 0.68 +0.0
551,854 - -0.1
551,901 - +0.2
551,937 + -1.1
552,264 - BWI76_RS02725 0.64 -1.7
552,409 + +1.2
552,437 - +0.2
552,688 + BWI76_RS02730 0.12 -0.8
553,210 + BWI76_RS02730 0.39 +1.2
553,225 + BWI76_RS02730 0.40 -0.4

Or see this region's nucleotide sequence