Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02260

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02250 and BWI76_RS02255 are separated by 121 nucleotidesBWI76_RS02255 and BWI76_RS02260 overlap by 1 nucleotidesBWI76_RS02260 and BWI76_RS02265 are separated by 149 nucleotides BWI76_RS02250: BWI76_RS02250 - proline/betaine transporter, at 462,856 to 464,358 _RS02250 BWI76_RS02255: BWI76_RS02255 - chaperone-modulator protein CbpM, at 464,480 to 464,785 _RS02255 BWI76_RS02260: BWI76_RS02260 - DNA-binding protein, at 464,785 to 465,705 _RS02260 BWI76_RS02265: BWI76_RS02265 - putative transmembrane protein, at 465,855 to 466,535 _RS02265 Position (kb) 464 465 466Strain fitness (log2 ratio) -1 0 1 2at 463.840 kb on - strand, within BWI76_RS02250at 463.999 kb on + strand, within BWI76_RS02250at 464.047 kb on - strand, within BWI76_RS02250at 464.069 kb on + strand, within BWI76_RS02250at 464.069 kb on + strand, within BWI76_RS02250at 464.096 kb on + strand, within BWI76_RS02250at 464.151 kb on + strand, within BWI76_RS02250at 464.326 kb on - strandat 464.447 kb on - strandat 464.614 kb on + strand, within BWI76_RS02255at 464.619 kb on - strand, within BWI76_RS02255at 464.632 kb on - strand, within BWI76_RS02255at 464.683 kb on + strand, within BWI76_RS02255at 464.801 kb on + strandat 464.831 kb on + strandat 464.934 kb on + strand, within BWI76_RS02260at 464.934 kb on + strand, within BWI76_RS02260at 465.097 kb on - strand, within BWI76_RS02260at 465.279 kb on + strand, within BWI76_RS02260at 465.435 kb on + strand, within BWI76_RS02260at 465.437 kb on + strand, within BWI76_RS02260at 465.445 kb on - strand, within BWI76_RS02260at 465.536 kb on - strand, within BWI76_RS02260at 465.608 kb on - strand, within BWI76_RS02260at 465.647 kb on - strandat 465.647 kb on - strandat 465.647 kb on - strandat 465.801 kb on - strandat 465.853 kb on + strandat 465.907 kb on - strandat 465.907 kb on - strandat 465.925 kb on - strand, within BWI76_RS02265at 466.201 kb on - strand, within BWI76_RS02265at 466.315 kb on - strand, within BWI76_RS02265at 466.421 kb on + strand, within BWI76_RS02265at 466.429 kb on - strand, within BWI76_RS02265at 466.434 kb on - strand, within BWI76_RS02265at 466.589 kb on + strandat 466.589 kb on + strandat 466.589 kb on + strandat 466.591 kb on - strandat 466.597 kb on - strandat 466.606 kb on - strandat 466.614 kb on + strandat 466.695 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
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463,840 - BWI76_RS02250 0.65 -1.0
463,999 + BWI76_RS02250 0.76 -1.0
464,047 - BWI76_RS02250 0.79 -1.5
464,069 + BWI76_RS02250 0.81 -0.8
464,069 + BWI76_RS02250 0.81 +0.4
464,096 + BWI76_RS02250 0.83 -0.3
464,151 + BWI76_RS02250 0.86 +0.7
464,326 - -0.5
464,447 - -0.2
464,614 + BWI76_RS02255 0.44 -0.0
464,619 - BWI76_RS02255 0.45 +0.9
464,632 - BWI76_RS02255 0.50 -0.3
464,683 + BWI76_RS02255 0.66 +0.4
464,801 + +0.9
464,831 + +0.4
464,934 + BWI76_RS02260 0.16 +1.1
464,934 + BWI76_RS02260 0.16 -0.8
465,097 - BWI76_RS02260 0.34 +0.2
465,279 + BWI76_RS02260 0.54 +0.3
465,435 + BWI76_RS02260 0.71 -1.3
465,437 + BWI76_RS02260 0.71 -1.3
465,445 - BWI76_RS02260 0.72 -1.2
465,536 - BWI76_RS02260 0.82 -0.5
465,608 - BWI76_RS02260 0.89 -0.7
465,647 - +0.0
465,647 - +0.9
465,647 - -0.5
465,801 - -0.2
465,853 + -0.7
465,907 - +0.6
465,907 - +0.7
465,925 - BWI76_RS02265 0.10 +0.8
466,201 - BWI76_RS02265 0.51 +0.1
466,315 - BWI76_RS02265 0.68 +0.6
466,421 + BWI76_RS02265 0.83 +0.4
466,429 - BWI76_RS02265 0.84 +2.1
466,434 - BWI76_RS02265 0.85 -0.9
466,589 + -0.2
466,589 + -1.2
466,589 + -0.0
466,591 - +1.1
466,597 - +0.5
466,606 - +0.1
466,614 + -0.5
466,695 + +0.6

Or see this region's nucleotide sequence