Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02250 and BWI76_RS02255 are separated by 121 nucleotides BWI76_RS02255 and BWI76_RS02260 overlap by 1 nucleotides BWI76_RS02260 and BWI76_RS02265 are separated by 149 nucleotides
BWI76_RS02250: BWI76_RS02250 - proline/betaine transporter, at 462,856 to 464,358
_RS02250
BWI76_RS02255: BWI76_RS02255 - chaperone-modulator protein CbpM, at 464,480 to 464,785
_RS02255
BWI76_RS02260: BWI76_RS02260 - DNA-binding protein, at 464,785 to 465,705
_RS02260
BWI76_RS02265: BWI76_RS02265 - putative transmembrane protein, at 465,855 to 466,535
_RS02265
Position (kb)
464
465
466 Strain fitness (log2 ratio)
-1
0
1
2 at 463.840 kb on - strand, within BWI76_RS02250 at 463.999 kb on + strand, within BWI76_RS02250 at 464.047 kb on - strand, within BWI76_RS02250 at 464.069 kb on + strand, within BWI76_RS02250 at 464.069 kb on + strand, within BWI76_RS02250 at 464.096 kb on + strand, within BWI76_RS02250 at 464.151 kb on + strand, within BWI76_RS02250 at 464.326 kb on - strand at 464.447 kb on - strand at 464.614 kb on + strand, within BWI76_RS02255 at 464.619 kb on - strand, within BWI76_RS02255 at 464.632 kb on - strand, within BWI76_RS02255 at 464.683 kb on + strand, within BWI76_RS02255 at 464.801 kb on + strand at 464.831 kb on + strand at 464.934 kb on + strand, within BWI76_RS02260 at 464.934 kb on + strand, within BWI76_RS02260 at 465.097 kb on - strand, within BWI76_RS02260 at 465.279 kb on + strand, within BWI76_RS02260 at 465.435 kb on + strand, within BWI76_RS02260 at 465.437 kb on + strand, within BWI76_RS02260 at 465.445 kb on - strand, within BWI76_RS02260 at 465.536 kb on - strand, within BWI76_RS02260 at 465.608 kb on - strand, within BWI76_RS02260 at 465.647 kb on - strand at 465.647 kb on - strand at 465.647 kb on - strand at 465.801 kb on - strand at 465.853 kb on + strand at 465.907 kb on - strand at 465.907 kb on - strand at 465.925 kb on - strand, within BWI76_RS02265 at 466.201 kb on - strand, within BWI76_RS02265 at 466.315 kb on - strand, within BWI76_RS02265 at 466.421 kb on + strand, within BWI76_RS02265 at 466.429 kb on - strand, within BWI76_RS02265 at 466.434 kb on - strand, within BWI76_RS02265 at 466.589 kb on + strand at 466.589 kb on + strand at 466.589 kb on + strand at 466.591 kb on - strand at 466.597 kb on - strand at 466.606 kb on - strand at 466.614 kb on + strand at 466.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 463,840 - BWI76_RS02250 0.65 -1.0 463,999 + BWI76_RS02250 0.76 -1.0 464,047 - BWI76_RS02250 0.79 -1.5 464,069 + BWI76_RS02250 0.81 -0.8 464,069 + BWI76_RS02250 0.81 +0.4 464,096 + BWI76_RS02250 0.83 -0.3 464,151 + BWI76_RS02250 0.86 +0.7 464,326 - -0.5 464,447 - -0.2 464,614 + BWI76_RS02255 0.44 -0.0 464,619 - BWI76_RS02255 0.45 +0.9 464,632 - BWI76_RS02255 0.50 -0.3 464,683 + BWI76_RS02255 0.66 +0.4 464,801 + +0.9 464,831 + +0.4 464,934 + BWI76_RS02260 0.16 +1.1 464,934 + BWI76_RS02260 0.16 -0.8 465,097 - BWI76_RS02260 0.34 +0.2 465,279 + BWI76_RS02260 0.54 +0.3 465,435 + BWI76_RS02260 0.71 -1.3 465,437 + BWI76_RS02260 0.71 -1.3 465,445 - BWI76_RS02260 0.72 -1.2 465,536 - BWI76_RS02260 0.82 -0.5 465,608 - BWI76_RS02260 0.89 -0.7 465,647 - +0.0 465,647 - +0.9 465,647 - -0.5 465,801 - -0.2 465,853 + -0.7 465,907 - +0.6 465,907 - +0.7 465,925 - BWI76_RS02265 0.10 +0.8 466,201 - BWI76_RS02265 0.51 +0.1 466,315 - BWI76_RS02265 0.68 +0.6 466,421 + BWI76_RS02265 0.83 +0.4 466,429 - BWI76_RS02265 0.84 +2.1 466,434 - BWI76_RS02265 0.85 -0.9 466,589 + -0.2 466,589 + -1.2 466,589 + -0.0 466,591 - +1.1 466,597 - +0.5 466,606 - +0.1 466,614 + -0.5 466,695 + +0.6
Or see this region's nucleotide sequence