Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01375 and BWI76_RS01380 overlap by 1 nucleotides BWI76_RS01380 and BWI76_RS01385 are separated by 41 nucleotides BWI76_RS01385 and BWI76_RS01390 are separated by 11 nucleotides BWI76_RS01390 and BWI76_RS01395 are separated by 387 nucleotides
BWI76_RS01375: BWI76_RS01375 - Xaa-Pro dipeptidase, at 281,018 to 282,349
_RS01375
BWI76_RS01380: BWI76_RS01380 - YigZ family protein, at 282,349 to 282,963
_RS01380
BWI76_RS01385: BWI76_RS01385 - potassium transporter, at 283,005 to 284,456
_RS01385
BWI76_RS01390: BWI76_RS01390 - protoporphyrinogen oxidase, at 284,468 to 285,001
_RS01390
BWI76_RS01395: BWI76_RS01395 - 16S ribosomal RNA, at 285,389 to 286,942
_RS01395
Position (kb) 283
284
285 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 282.032 kb on - strand, within BWI76_RS01375 at 282.032 kb on - strand, within BWI76_RS01375 at 282.076 kb on + strand, within BWI76_RS01375 at 282.095 kb on - strand, within BWI76_RS01375 at 282.095 kb on - strand, within BWI76_RS01375 at 282.099 kb on - strand, within BWI76_RS01375 at 282.100 kb on - strand, within BWI76_RS01375 at 282.220 kb on + strand at 282.230 kb on + strand at 282.479 kb on - strand, within BWI76_RS01380 at 282.591 kb on + strand, within BWI76_RS01380 at 282.719 kb on + strand, within BWI76_RS01380 at 282.720 kb on - strand, within BWI76_RS01380 at 283.237 kb on - strand, within BWI76_RS01385 at 283.264 kb on + strand, within BWI76_RS01385 at 283.908 kb on - strand, within BWI76_RS01385 at 283.931 kb on + strand, within BWI76_RS01385 at 284.107 kb on - strand, within BWI76_RS01385 at 284.314 kb on + strand at 284.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 282,032 - BWI76_RS01375 0.76 -0.5 282,032 - BWI76_RS01375 0.76 -0.5 282,076 + BWI76_RS01375 0.79 +0.8 282,095 - BWI76_RS01375 0.81 +0.3 282,095 - BWI76_RS01375 0.81 +1.4 282,099 - BWI76_RS01375 0.81 -2.7 282,100 - BWI76_RS01375 0.81 +0.1 282,220 + +1.9 282,230 + +2.4 282,479 - BWI76_RS01380 0.21 -0.6 282,591 + BWI76_RS01380 0.39 -3.0 282,719 + BWI76_RS01380 0.60 +0.2 282,720 - BWI76_RS01380 0.60 -0.5 283,237 - BWI76_RS01385 0.16 +0.1 283,264 + BWI76_RS01385 0.18 -1.5 283,908 - BWI76_RS01385 0.62 +0.1 283,931 + BWI76_RS01385 0.64 -2.5 284,107 - BWI76_RS01385 0.76 -1.1 284,314 + -0.3 284,460 - +0.8
Or see this region's nucleotide sequence