Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01180 and BWI76_RS01185 are separated by 346 nucleotides BWI76_RS01185 and BWI76_RS01190 are separated by 80 nucleotides BWI76_RS01190 and BWI76_RS01195 are separated by 138 nucleotides BWI76_RS01195 and BWI76_RS01200 are separated by 25 nucleotides
BWI76_RS01180: BWI76_RS01180 - porphobilinogen deaminase, at 240,754 to 241,695
_RS01180
BWI76_RS01185: BWI76_RS01185 - adenylate cyclase, at 242,042 to 244,591
_RS01185
BWI76_RS01190: BWI76_RS01190 - iron donor protein CyaY, at 244,672 to 244,992
_RS01190
BWI76_RS01195: BWI76_RS01195 - membrane protein, at 245,131 to 245,334
_RS01195
BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184
_RS01200
Position (kb) 242
243
244
245 Strain fitness (log2 ratio) -2
-1
0
1 at 241.724 kb on - strand at 241.765 kb on + strand at 241.857 kb on - strand at 241.938 kb on + strand at 242.047 kb on + strand at 242.055 kb on - strand at 243.014 kb on - strand, within BWI76_RS01185 at 243.445 kb on - strand, within BWI76_RS01185 at 243.456 kb on + strand, within BWI76_RS01185 at 243.956 kb on - strand, within BWI76_RS01185 at 243.956 kb on - strand, within BWI76_RS01185 at 244.498 kb on + strand at 244.555 kb on - strand at 244.608 kb on + strand at 244.876 kb on - strand, within BWI76_RS01190 at 244.920 kb on - strand, within BWI76_RS01190 at 245.186 kb on + strand, within BWI76_RS01195 at 245.194 kb on - strand, within BWI76_RS01195 at 245.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 241,724 - +0.6 241,765 + -0.1 241,857 - -0.4 241,938 + -0.1 242,047 + +0.1 242,055 - +1.1 243,014 - BWI76_RS01185 0.38 +1.1 243,445 - BWI76_RS01185 0.55 +1.1 243,456 + BWI76_RS01185 0.55 -0.5 243,956 - BWI76_RS01185 0.75 -2.2 243,956 - BWI76_RS01185 0.75 +0.1 244,498 + -0.4 244,555 - -0.5 244,608 + +1.0 244,876 - BWI76_RS01190 0.64 +0.2 244,920 - BWI76_RS01190 0.77 -0.1 245,186 + BWI76_RS01195 0.27 -1.0 245,194 - BWI76_RS01195 0.31 +1.1 245,337 + -0.4
Or see this region's nucleotide sequence