Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01105

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01100 and BWI76_RS01105 are separated by 18 nucleotidesBWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotidesBWI76_RS01110 and BWI76_RS01115 are separated by 4 nucleotides BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887 _RS01100 BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787 _RS01105 BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439 _RS01110 BWI76_RS01115: BWI76_RS01115 - dTDP-4-amino-4,6-dideoxygalactose transaminase, at 229,444 to 230,574 _RS01115 Position (kb) 227 228 229Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 226.918 kb on + strandat 226.979 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.140 kb on - strand, within BWI76_RS01100at 227.142 kb on + strand, within BWI76_RS01100at 227.227 kb on + strand, within BWI76_RS01100at 227.235 kb on - strand, within BWI76_RS01100at 227.642 kb on - strand, within BWI76_RS01100at 227.921 kb on - strandat 228.196 kb on - strand, within BWI76_RS01105at 228.249 kb on + strand, within BWI76_RS01105at 228.263 kb on - strand, within BWI76_RS01105at 228.273 kb on - strand, within BWI76_RS01105at 228.384 kb on + strand, within BWI76_RS01105at 228.654 kb on + strand, within BWI76_RS01105at 228.789 kb on - strandat 228.839 kb on - strand, within BWI76_RS01110at 228.927 kb on + strand, within BWI76_RS01110at 229.276 kb on - strand, within BWI76_RS01110at 229.279 kb on + strand, within BWI76_RS01110at 229.315 kb on + strand, within BWI76_RS01110at 229.536 kb on - strandat 229.602 kb on + strand, within BWI76_RS01115at 229.711 kb on - strand, within BWI76_RS01115at 229.716 kb on - strand, within BWI76_RS01115

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
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226,918 + -0.9
226,979 + BWI76_RS01100 0.15 -0.2
227,132 + BWI76_RS01100 0.29 -0.1
227,132 + BWI76_RS01100 0.29 -0.2
227,132 + BWI76_RS01100 0.29 +1.1
227,140 - BWI76_RS01100 0.30 -1.9
227,142 + BWI76_RS01100 0.30 -0.2
227,227 + BWI76_RS01100 0.38 +0.1
227,235 - BWI76_RS01100 0.39 -1.2
227,642 - BWI76_RS01100 0.77 -2.5
227,921 - -0.8
228,196 - BWI76_RS01105 0.33 -2.3
228,249 + BWI76_RS01105 0.39 -0.3
228,263 - BWI76_RS01105 0.40 -1.2
228,273 - BWI76_RS01105 0.42 -2.2
228,384 + BWI76_RS01105 0.54 -1.0
228,654 + BWI76_RS01105 0.85 -1.2
228,789 - -3.2
228,839 - BWI76_RS01110 0.11 -1.4
228,927 + BWI76_RS01110 0.24 +1.1
229,276 - BWI76_RS01110 0.76 -2.0
229,279 + BWI76_RS01110 0.76 -2.6
229,315 + BWI76_RS01110 0.81 -4.3
229,536 - -3.3
229,602 + BWI76_RS01115 0.14 -2.7
229,711 - BWI76_RS01115 0.24 -0.4
229,716 - BWI76_RS01115 0.24 -1.3

Or see this region's nucleotide sequence