Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26760 and BWI76_RS26765 are separated by 154 nucleotides BWI76_RS26765 and BWI76_RS26770 are separated by 436 nucleotides
BWI76_RS26760: BWI76_RS26760 - C4-dicarboxylate transport protein DctA <small>(from data)</small>, at 5,542,491 to 5,543,777
_RS26760
BWI76_RS26765: BWI76_RS26765 - phosphodiesterase, at 5,543,932 to 5,545,938
_RS26765
BWI76_RS26770: BWI76_RS26770 - cellulose biosynthesis protein BcsC, at 5,546,375 to 5,549,860
_RS26770
Position (kb) 5543
5544
5545
5546 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5543.274 kb on + strand at 5543.321 kb on - strand, within BWI76_RS26760 at 5543.402 kb on - strand, within BWI76_RS26760 at 5543.446 kb on + strand, within BWI76_RS26760 at 5543.513 kb on + strand, within BWI76_RS26760 at 5543.516 kb on + strand, within BWI76_RS26760 at 5543.554 kb on - strand, within BWI76_RS26760 at 5543.616 kb on + strand, within BWI76_RS26760 at 5543.648 kb on + strand, within BWI76_RS26760 at 5543.672 kb on - strand at 5543.738 kb on - strand at 5543.753 kb on + strand at 5543.851 kb on - strand at 5544.009 kb on - strand at 5544.114 kb on + strand at 5544.122 kb on - strand at 5544.230 kb on - strand, within BWI76_RS26765 at 5544.235 kb on - strand, within BWI76_RS26765 at 5544.240 kb on - strand, within BWI76_RS26765 at 5544.614 kb on - strand, within BWI76_RS26765 at 5544.636 kb on - strand, within BWI76_RS26765 at 5544.735 kb on + strand, within BWI76_RS26765 at 5544.743 kb on - strand, within BWI76_RS26765 at 5544.801 kb on + strand, within BWI76_RS26765 at 5544.879 kb on - strand, within BWI76_RS26765 at 5544.881 kb on - strand, within BWI76_RS26765 at 5545.043 kb on + strand, within BWI76_RS26765 at 5545.096 kb on - strand, within BWI76_RS26765 at 5545.171 kb on + strand, within BWI76_RS26765 at 5545.223 kb on - strand, within BWI76_RS26765 at 5545.562 kb on - strand, within BWI76_RS26765 at 5545.675 kb on + strand, within BWI76_RS26765 at 5545.735 kb on - strand, within BWI76_RS26765 at 5545.735 kb on - strand, within BWI76_RS26765 at 5545.756 kb on - strand at 5545.795 kb on + strand at 5545.859 kb on - strand at 5545.859 kb on - strand at 5546.102 kb on + strand at 5546.483 kb on - strand at 5546.592 kb on + strand at 5546.613 kb on + strand at 5546.661 kb on + strand at 5546.669 kb on - strand at 5546.746 kb on - strand, within BWI76_RS26770 at 5546.757 kb on + strand, within BWI76_RS26770 at 5546.802 kb on - strand, within BWI76_RS26770 at 5546.802 kb on - strand, within BWI76_RS26770 at 5546.802 kb on - strand, within BWI76_RS26770
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,543,274 + +0.5 5,543,321 - BWI76_RS26760 0.64 +0.5 5,543,402 - BWI76_RS26760 0.71 +0.4 5,543,446 + BWI76_RS26760 0.74 +1.1 5,543,513 + BWI76_RS26760 0.79 +0.6 5,543,516 + BWI76_RS26760 0.80 +0.7 5,543,554 - BWI76_RS26760 0.83 -0.5 5,543,616 + BWI76_RS26760 0.87 +0.9 5,543,648 + BWI76_RS26760 0.90 +0.1 5,543,672 - +0.2 5,543,738 - -0.1 5,543,753 + -1.2 5,543,851 - +0.4 5,544,009 - -1.2 5,544,114 + -0.8 5,544,122 - -0.9 5,544,230 - BWI76_RS26765 0.15 -0.5 5,544,235 - BWI76_RS26765 0.15 -0.6 5,544,240 - BWI76_RS26765 0.15 -0.9 5,544,614 - BWI76_RS26765 0.34 -0.7 5,544,636 - BWI76_RS26765 0.35 -0.4 5,544,735 + BWI76_RS26765 0.40 -0.6 5,544,743 - BWI76_RS26765 0.40 +0.1 5,544,801 + BWI76_RS26765 0.43 -2.7 5,544,879 - BWI76_RS26765 0.47 -0.5 5,544,881 - BWI76_RS26765 0.47 -1.8 5,545,043 + BWI76_RS26765 0.55 -1.0 5,545,096 - BWI76_RS26765 0.58 -1.3 5,545,171 + BWI76_RS26765 0.62 -1.5 5,545,223 - BWI76_RS26765 0.64 -1.4 5,545,562 - BWI76_RS26765 0.81 -1.3 5,545,675 + BWI76_RS26765 0.87 -2.1 5,545,735 - BWI76_RS26765 0.90 -2.4 5,545,735 - BWI76_RS26765 0.90 -0.5 5,545,756 - -2.3 5,545,795 + -2.5 5,545,859 - -2.7 5,545,859 - -1.5 5,546,102 + +1.1 5,546,483 - +0.4 5,546,592 + -0.3 5,546,613 + -1.7 5,546,661 + -0.8 5,546,669 - +1.1 5,546,746 - BWI76_RS26770 0.11 +2.2 5,546,757 + BWI76_RS26770 0.11 +1.1 5,546,802 - BWI76_RS26770 0.12 +0.6 5,546,802 - BWI76_RS26770 0.12 -0.1 5,546,802 - BWI76_RS26770 0.12 -1.0
Or see this region's nucleotide sequence