Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26615 and BWI76_RS26620 are separated by 331 nucleotides BWI76_RS26620 and BWI76_RS26625 are separated by 58 nucleotides BWI76_RS26625 and BWI76_RS26630 are separated by 37 nucleotides
BWI76_RS26615: BWI76_RS26615 - gluconate:H+ symporter <small>(from data)</small>, at 5,510,053 to 5,511,369
_RS26615
BWI76_RS26620: BWI76_RS26620 - Fe-S biogenesis protein NfuA, at 5,511,701 to 5,512,276
_RS26620
BWI76_RS26625: BWI76_RS26625 - gluconate transporter periplasmic gluconate-binding protein, at 5,512,335 to 5,513,009
_RS26625
BWI76_RS26630: BWI76_RS26630 - pimeloyl-[acyl-carrier protein] methyl ester esterase, at 5,513,047 to 5,513,829
_RS26630
Position (kb) 5511
5512
5513 Strain fitness (log2 ratio) -2
-1
0
1 at 5510.844 kb on + strand, within BWI76_RS26615 at 5510.848 kb on + strand, within BWI76_RS26615 at 5510.922 kb on - strand, within BWI76_RS26615 at 5511.026 kb on + strand, within BWI76_RS26615 at 5511.031 kb on + strand, within BWI76_RS26615 at 5511.253 kb on - strand at 5511.370 kb on + strand at 5511.658 kb on - strand at 5511.659 kb on + strand at 5511.664 kb on - strand at 5512.114 kb on - strand, within BWI76_RS26620 at 5512.362 kb on - strand at 5512.511 kb on + strand, within BWI76_RS26625 at 5512.734 kb on + strand, within BWI76_RS26625 at 5512.743 kb on - strand, within BWI76_RS26625 at 5512.770 kb on - strand, within BWI76_RS26625 at 5512.860 kb on + strand, within BWI76_RS26625 at 5513.030 kb on + strand at 5513.108 kb on + strand at 5513.138 kb on + strand, within BWI76_RS26630 at 5513.141 kb on - strand, within BWI76_RS26630
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,510,844 + BWI76_RS26615 0.60 +0.3 5,510,848 + BWI76_RS26615 0.60 +0.9 5,510,922 - BWI76_RS26615 0.66 +0.8 5,511,026 + BWI76_RS26615 0.74 +1.0 5,511,031 + BWI76_RS26615 0.74 -1.5 5,511,253 - -0.7 5,511,370 + -1.5 5,511,658 - +1.1 5,511,659 + +0.2 5,511,664 - -0.5 5,512,114 - BWI76_RS26620 0.72 -2.0 5,512,362 - -1.2 5,512,511 + BWI76_RS26625 0.26 +0.6 5,512,734 + BWI76_RS26625 0.59 -0.9 5,512,743 - BWI76_RS26625 0.60 -0.1 5,512,770 - BWI76_RS26625 0.64 +0.5 5,512,860 + BWI76_RS26625 0.78 +0.2 5,513,030 + -0.1 5,513,108 + -0.6 5,513,138 + BWI76_RS26630 0.12 -0.8 5,513,141 - BWI76_RS26630 0.12 -0.1
Or see this region's nucleotide sequence