Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25975 and BWI76_RS25980 are separated by 46 nucleotides BWI76_RS25980 and BWI76_RS25985 overlap by 41 nucleotides BWI76_RS25985 and BWI76_RS25990 are separated by 157 nucleotides BWI76_RS25990 and BWI76_RS25995 are separated by 51 nucleotides
BWI76_RS25975: BWI76_RS25975 - phosphoribulokinase, at 5,369,470 to 5,370,339
_RS25975
BWI76_RS25980: BWI76_RS25980 - hypothetical protein, at 5,370,386 to 5,370,790
_RS25980
BWI76_RS25985: BWI76_RS25985 - hypothetical protein, at 5,370,750 to 5,370,937
_RS25985
BWI76_RS25990: BWI76_RS25990 - cAMP-activated global transcriptional regulator CRP, at 5,371,095 to 5,371,727
_RS25990
BWI76_RS25995: BWI76_RS25995 - membrane protein, at 5,371,779 to 5,373,857
_RS25995
Position (kb) 5370
5371 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5369.868 kb on - strand, within BWI76_RS25975 at 5370.107 kb on + strand, within BWI76_RS25975 at 5370.115 kb on - strand, within BWI76_RS25975 at 5370.493 kb on - strand, within BWI76_RS25980 at 5370.493 kb on - strand, within BWI76_RS25980 at 5370.569 kb on + strand, within BWI76_RS25980 at 5370.968 kb on + strand at 5370.971 kb on - strand at 5370.992 kb on + strand at 5371.814 kb on - strand at 5371.890 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,369,868 - BWI76_RS25975 0.46 -0.7 5,370,107 + BWI76_RS25975 0.73 -1.8 5,370,115 - BWI76_RS25975 0.74 -3.1 5,370,493 - BWI76_RS25980 0.26 +1.0 5,370,493 - BWI76_RS25980 0.26 +0.6 5,370,569 + BWI76_RS25980 0.45 +0.1 5,370,968 + +0.1 5,370,971 - +0.1 5,370,992 + +0.7 5,371,814 - +0.1 5,371,890 + -0.2
Or see this region's nucleotide sequence