Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25965 and BWI76_RS25970 overlap by 4 nucleotides BWI76_RS25970 and BWI76_RS25975 are separated by 35 nucleotides BWI76_RS25975 and BWI76_RS25980 are separated by 46 nucleotides BWI76_RS25980 and BWI76_RS25985 overlap by 41 nucleotides BWI76_RS25985 and BWI76_RS25990 are separated by 157 nucleotides
BWI76_RS25965: BWI76_RS25965 - hydrolase, at 5,368,197 to 5,369,219
_RS25965
BWI76_RS25970: BWI76_RS25970 - hypothetical protein, at 5,369,216 to 5,369,434
_RS25970
BWI76_RS25975: BWI76_RS25975 - phosphoribulokinase, at 5,369,470 to 5,370,339
_RS25975
BWI76_RS25980: BWI76_RS25980 - hypothetical protein, at 5,370,386 to 5,370,790
_RS25980
BWI76_RS25985: BWI76_RS25985 - hypothetical protein, at 5,370,750 to 5,370,937
_RS25985
BWI76_RS25990: BWI76_RS25990 - cAMP-activated global transcriptional regulator CRP, at 5,371,095 to 5,371,727
_RS25990
Position (kb) 5369
5370
5371 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5368.640 kb on - strand, within BWI76_RS25965 at 5368.806 kb on + strand, within BWI76_RS25965 at 5368.858 kb on + strand, within BWI76_RS25965 at 5368.937 kb on - strand, within BWI76_RS25965 at 5368.937 kb on - strand, within BWI76_RS25965 at 5369.035 kb on - strand, within BWI76_RS25965 at 5369.271 kb on - strand, within BWI76_RS25970 at 5369.314 kb on - strand, within BWI76_RS25970 at 5369.379 kb on + strand, within BWI76_RS25970 at 5369.392 kb on - strand, within BWI76_RS25970 at 5369.392 kb on - strand, within BWI76_RS25970 at 5369.503 kb on + strand at 5369.503 kb on + strand at 5369.711 kb on - strand, within BWI76_RS25975 at 5369.746 kb on - strand, within BWI76_RS25975 at 5369.868 kb on - strand, within BWI76_RS25975 at 5370.107 kb on + strand, within BWI76_RS25975 at 5370.115 kb on - strand, within BWI76_RS25975 at 5370.493 kb on - strand, within BWI76_RS25980 at 5370.493 kb on - strand, within BWI76_RS25980 at 5370.569 kb on + strand, within BWI76_RS25980 at 5370.968 kb on + strand at 5370.971 kb on - strand at 5370.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,368,640 - BWI76_RS25965 0.43 +0.3 5,368,806 + BWI76_RS25965 0.60 -0.2 5,368,858 + BWI76_RS25965 0.65 +0.3 5,368,937 - BWI76_RS25965 0.72 -0.5 5,368,937 - BWI76_RS25965 0.72 -0.4 5,369,035 - BWI76_RS25965 0.82 -0.3 5,369,271 - BWI76_RS25970 0.25 +1.0 5,369,314 - BWI76_RS25970 0.45 +0.4 5,369,379 + BWI76_RS25970 0.74 -0.8 5,369,392 - BWI76_RS25970 0.80 -1.2 5,369,392 - BWI76_RS25970 0.80 +0.1 5,369,503 + +1.2 5,369,503 + -0.3 5,369,711 - BWI76_RS25975 0.28 -0.1 5,369,746 - BWI76_RS25975 0.32 -0.9 5,369,868 - BWI76_RS25975 0.46 -0.7 5,370,107 + BWI76_RS25975 0.73 -1.8 5,370,115 - BWI76_RS25975 0.74 -3.1 5,370,493 - BWI76_RS25980 0.26 +1.0 5,370,493 - BWI76_RS25980 0.26 +0.6 5,370,569 + BWI76_RS25980 0.45 +0.1 5,370,968 + +0.1 5,370,971 - +0.1 5,370,992 + +0.7
Or see this region's nucleotide sequence