Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24905 and BWI76_RS24910 are separated by 4 nucleotides BWI76_RS24910 and BWI76_RS24915 are separated by 63 nucleotides
BWI76_RS24905: BWI76_RS24905 - filamentation induced by cAMP protein fic, at 5,189,505 to 5,190,506
_RS24905
BWI76_RS24910: BWI76_RS24910 - rRNA (cytidine-2'-O-)-methyltransferase, at 5,190,511 to 5,191,374
_RS24910
BWI76_RS24915: BWI76_RS24915 - penicillin-binding protein activator, at 5,191,438 to 5,193,519
_RS24915
Position (kb)
5190
5191
5192 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5189.620 kb on + strand, within BWI76_RS24905 at 5189.627 kb on - strand, within BWI76_RS24905 at 5189.830 kb on - strand, within BWI76_RS24905 at 5189.844 kb on - strand, within BWI76_RS24905 at 5189.921 kb on + strand, within BWI76_RS24905 at 5189.976 kb on + strand, within BWI76_RS24905 at 5189.994 kb on + strand, within BWI76_RS24905 at 5190.067 kb on + strand, within BWI76_RS24905 at 5190.123 kb on + strand, within BWI76_RS24905 at 5190.300 kb on - strand, within BWI76_RS24905 at 5190.344 kb on - strand, within BWI76_RS24905 at 5190.344 kb on - strand, within BWI76_RS24905 at 5190.406 kb on + strand at 5190.410 kb on - strand at 5190.468 kb on + strand at 5190.481 kb on - strand at 5190.508 kb on + strand at 5190.517 kb on - strand at 5190.523 kb on - strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.536 kb on + strand at 5190.544 kb on - strand at 5190.544 kb on - strand at 5190.699 kb on - strand, within BWI76_RS24910 at 5190.911 kb on - strand, within BWI76_RS24910 at 5191.009 kb on + strand, within BWI76_RS24910 at 5191.010 kb on + strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.125 kb on - strand, within BWI76_RS24910 at 5191.127 kb on + strand, within BWI76_RS24910 at 5191.135 kb on - strand, within BWI76_RS24910 at 5191.275 kb on + strand, within BWI76_RS24910 at 5191.283 kb on - strand, within BWI76_RS24910 at 5191.319 kb on - strand at 5191.336 kb on - strand at 5191.336 kb on - strand at 5191.721 kb on - strand, within BWI76_RS24915 at 5191.935 kb on - strand, within BWI76_RS24915 at 5191.978 kb on + strand, within BWI76_RS24915 at 5192.049 kb on - strand, within BWI76_RS24915 at 5192.064 kb on - strand, within BWI76_RS24915 at 5192.195 kb on - strand, within BWI76_RS24915 at 5192.334 kb on + strand, within BWI76_RS24915
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,189,620 + BWI76_RS24905 0.11 +0.3 5,189,627 - BWI76_RS24905 0.12 +1.0 5,189,830 - BWI76_RS24905 0.32 +0.3 5,189,844 - BWI76_RS24905 0.34 +0.6 5,189,921 + BWI76_RS24905 0.42 -0.3 5,189,976 + BWI76_RS24905 0.47 -0.3 5,189,994 + BWI76_RS24905 0.49 -0.0 5,190,067 + BWI76_RS24905 0.56 -1.3 5,190,123 + BWI76_RS24905 0.62 -0.1 5,190,300 - BWI76_RS24905 0.79 -0.9 5,190,344 - BWI76_RS24905 0.84 -0.1 5,190,344 - BWI76_RS24905 0.84 -0.0 5,190,406 + +0.2 5,190,410 - +0.1 5,190,468 + -0.5 5,190,481 - +1.1 5,190,508 + -0.5 5,190,517 - -0.3 5,190,523 - +0.3 5,190,536 + +0.4 5,190,536 + -0.9 5,190,536 + +0.2 5,190,544 - -0.1 5,190,544 - -2.2 5,190,699 - BWI76_RS24910 0.22 -0.7 5,190,911 - BWI76_RS24910 0.46 -3.3 5,191,009 + BWI76_RS24910 0.58 -2.0 5,191,010 + BWI76_RS24910 0.58 -0.9 5,191,125 - BWI76_RS24910 0.71 +0.5 5,191,125 - BWI76_RS24910 0.71 -1.3 5,191,127 + BWI76_RS24910 0.71 -2.6 5,191,135 - BWI76_RS24910 0.72 +1.1 5,191,275 + BWI76_RS24910 0.88 +0.1 5,191,283 - BWI76_RS24910 0.89 -1.3 5,191,319 - +0.3 5,191,336 - -0.7 5,191,336 - -1.9 5,191,721 - BWI76_RS24915 0.14 -1.3 5,191,935 - BWI76_RS24915 0.24 -1.5 5,191,978 + BWI76_RS24915 0.26 +0.9 5,192,049 - BWI76_RS24915 0.29 +0.5 5,192,064 - BWI76_RS24915 0.30 -0.2 5,192,195 - BWI76_RS24915 0.36 -0.5 5,192,334 + BWI76_RS24915 0.43 +0.8
Or see this region's nucleotide sequence