Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24595 and BWI76_RS24600 are separated by 10 nucleotides BWI76_RS24600 and BWI76_RS24605 are separated by 10 nucleotides
BWI76_RS24595: BWI76_RS24595 - dihydroxyacetone kinase subunit DhaK, at 5,126,406 to 5,127,476
_RS24595
BWI76_RS24600: BWI76_RS24600 - dihydroxyacetone kinase, at 5,127,487 to 5,128,119
_RS24600
BWI76_RS24605: BWI76_RS24605 - dihydroxyacetone kinase subunit DhaM, at 5,128,130 to 5,129,551
_RS24605
Position (kb) 5127
5128
5129 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 5126.725 kb on - strand, within BWI76_RS24595 at 5126.783 kb on + strand, within BWI76_RS24595 at 5126.855 kb on + strand, within BWI76_RS24595 at 5126.976 kb on - strand, within BWI76_RS24595 at 5127.021 kb on - strand, within BWI76_RS24595 at 5127.184 kb on - strand, within BWI76_RS24595 at 5127.384 kb on - strand at 5127.401 kb on + strand at 5127.444 kb on + strand at 5127.444 kb on + strand at 5127.452 kb on - strand at 5127.456 kb on + strand at 5127.456 kb on + strand at 5127.464 kb on - strand at 5127.464 kb on - strand at 5127.526 kb on - strand at 5127.624 kb on + strand, within BWI76_RS24600 at 5127.940 kb on - strand, within BWI76_RS24600 at 5128.013 kb on - strand, within BWI76_RS24600 at 5128.087 kb on + strand at 5128.266 kb on + strand at 5128.357 kb on - strand, within BWI76_RS24605 at 5128.375 kb on + strand, within BWI76_RS24605 at 5128.419 kb on - strand, within BWI76_RS24605 at 5128.437 kb on - strand, within BWI76_RS24605 at 5128.650 kb on + strand, within BWI76_RS24605 at 5128.676 kb on + strand, within BWI76_RS24605 at 5128.773 kb on + strand, within BWI76_RS24605 at 5128.773 kb on + strand, within BWI76_RS24605 at 5128.781 kb on - strand, within BWI76_RS24605 at 5128.918 kb on + strand, within BWI76_RS24605 at 5128.931 kb on - strand, within BWI76_RS24605 at 5129.094 kb on - strand, within BWI76_RS24605
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,126,725 - BWI76_RS24595 0.30 +0.2 5,126,783 + BWI76_RS24595 0.35 -0.3 5,126,855 + BWI76_RS24595 0.42 -1.7 5,126,976 - BWI76_RS24595 0.53 -2.2 5,127,021 - BWI76_RS24595 0.57 -2.4 5,127,184 - BWI76_RS24595 0.73 -1.2 5,127,384 - -0.5 5,127,401 + -1.1 5,127,444 + -1.2 5,127,444 + +0.7 5,127,452 - -1.5 5,127,456 + -0.3 5,127,456 + -0.3 5,127,464 - +0.2 5,127,464 - +1.1 5,127,526 - -0.9 5,127,624 + BWI76_RS24600 0.22 -1.5 5,127,940 - BWI76_RS24600 0.72 -0.5 5,128,013 - BWI76_RS24600 0.83 -3.4 5,128,087 + +1.0 5,128,266 + +0.1 5,128,357 - BWI76_RS24605 0.16 -0.9 5,128,375 + BWI76_RS24605 0.17 +0.1 5,128,419 - BWI76_RS24605 0.20 -0.9 5,128,437 - BWI76_RS24605 0.22 -2.7 5,128,650 + BWI76_RS24605 0.37 -0.5 5,128,676 + BWI76_RS24605 0.38 +1.1 5,128,773 + BWI76_RS24605 0.45 +1.1 5,128,773 + BWI76_RS24605 0.45 -4.1 5,128,781 - BWI76_RS24605 0.46 -0.5 5,128,918 + BWI76_RS24605 0.55 -1.2 5,128,931 - BWI76_RS24605 0.56 -2.7 5,129,094 - BWI76_RS24605 0.68 -0.9
Or see this region's nucleotide sequence