Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23115 and BWI76_RS23120 are separated by 10 nucleotides BWI76_RS23120 and BWI76_RS23125 are separated by 14 nucleotides BWI76_RS23125 and BWI76_RS23130 are separated by 15 nucleotides
BWI76_RS23115: BWI76_RS23115 - propanediol dehydratase large subunit, at 4,818,711 to 4,820,375
_RS23115
BWI76_RS23120: BWI76_RS23120 - propanediol dehydratase medium subunit, at 4,820,386 to 4,821,045
_RS23120
BWI76_RS23125: BWI76_RS23125 - propanediol dehydratase small subunit, at 4,821,060 to 4,821,581
_RS23125
BWI76_RS23130: BWI76_RS23130 - diol dehydratase reactivase subunit alpha, at 4,821,597 to 4,823,429
_RS23130
Position (kb) 4821
4822 Strain fitness (log2 ratio) -2
-1
0
1 at 4820.086 kb on - strand, within BWI76_RS23115 at 4820.093 kb on + strand, within BWI76_RS23115 at 4820.174 kb on - strand, within BWI76_RS23115 at 4820.192 kb on + strand, within BWI76_RS23115 at 4820.222 kb on - strand at 4820.350 kb on + strand at 4820.352 kb on + strand at 4820.458 kb on - strand, within BWI76_RS23120 at 4820.851 kb on + strand, within BWI76_RS23120 at 4820.920 kb on + strand, within BWI76_RS23120 at 4821.096 kb on + strand at 4821.290 kb on + strand, within BWI76_RS23125 at 4821.448 kb on - strand, within BWI76_RS23125 at 4821.582 kb on + strand at 4821.919 kb on + strand, within BWI76_RS23130 at 4821.951 kb on - strand, within BWI76_RS23130 at 4822.237 kb on - strand, within BWI76_RS23130 at 4822.237 kb on - strand, within BWI76_RS23130 at 4822.242 kb on + strand, within BWI76_RS23130 at 4822.466 kb on - strand, within BWI76_RS23130 at 4822.533 kb on + strand, within BWI76_RS23130 at 4822.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,820,086 - BWI76_RS23115 0.83 +0.6 4,820,093 + BWI76_RS23115 0.83 -1.0 4,820,174 - BWI76_RS23115 0.88 +0.3 4,820,192 + BWI76_RS23115 0.89 +0.2 4,820,222 - +0.9 4,820,350 + -0.3 4,820,352 + +0.1 4,820,458 - BWI76_RS23120 0.11 -0.0 4,820,851 + BWI76_RS23120 0.70 +0.6 4,820,920 + BWI76_RS23120 0.81 +0.0 4,821,096 + -1.3 4,821,290 + BWI76_RS23125 0.44 -2.6 4,821,448 - BWI76_RS23125 0.74 -1.2 4,821,582 + -2.6 4,821,919 + BWI76_RS23130 0.18 +0.3 4,821,951 - BWI76_RS23130 0.19 -0.5 4,822,237 - BWI76_RS23130 0.35 -2.7 4,822,237 - BWI76_RS23130 0.35 -0.0 4,822,242 + BWI76_RS23130 0.35 -0.4 4,822,466 - BWI76_RS23130 0.47 -1.5 4,822,533 + BWI76_RS23130 0.51 +0.3 4,822,538 + -0.6
Or see this region's nucleotide sequence