Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22210 and BWI76_RS22215 are separated by 262 nucleotides BWI76_RS22215 and BWI76_RS22220 overlap by 4 nucleotides BWI76_RS22220 and BWI76_RS22225 are separated by 13 nucleotides
BWI76_RS22210: BWI76_RS22210 - murein transglycosylase B, at 4,630,785 to 4,631,861
_RS22210
BWI76_RS22215: BWI76_RS22215 - PTS sorbitol transporter subunit IIC, at 4,632,124 to 4,632,687
_RS22215
BWI76_RS22220: BWI76_RS22220 - PTS glucitol/sorbitol transporter subunit IIB, at 4,632,684 to 4,633,661
_RS22220
BWI76_RS22225: BWI76_RS22225 - PTS sorbitol transporter subunit IIA, at 4,633,675 to 4,634,037
_RS22225
Position (kb) 4632
4633 Strain fitness (log2 ratio) -2
-1
0
1 at 4631.308 kb on - strand, within BWI76_RS22210 at 4631.423 kb on + strand, within BWI76_RS22210 at 4631.433 kb on - strand, within BWI76_RS22210 at 4631.443 kb on + strand, within BWI76_RS22210 at 4631.453 kb on - strand, within BWI76_RS22210 at 4631.473 kb on - strand, within BWI76_RS22210 at 4631.795 kb on - strand at 4631.928 kb on - strand at 4632.049 kb on - strand at 4632.331 kb on + strand, within BWI76_RS22215 at 4632.390 kb on + strand, within BWI76_RS22215 at 4632.427 kb on - strand, within BWI76_RS22215 at 4632.560 kb on + strand, within BWI76_RS22215 at 4632.994 kb on - strand, within BWI76_RS22220 at 4633.055 kb on + strand, within BWI76_RS22220 at 4633.335 kb on - strand, within BWI76_RS22220 at 4633.346 kb on - strand, within BWI76_RS22220 at 4633.415 kb on + strand, within BWI76_RS22220 at 4633.551 kb on + strand, within BWI76_RS22220 at 4633.559 kb on - strand, within BWI76_RS22220 at 4633.559 kb on - strand, within BWI76_RS22220
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,631,308 - BWI76_RS22210 0.49 +0.0 4,631,423 + BWI76_RS22210 0.59 -1.4 4,631,433 - BWI76_RS22210 0.60 -0.0 4,631,443 + BWI76_RS22210 0.61 -0.2 4,631,453 - BWI76_RS22210 0.62 +0.6 4,631,473 - BWI76_RS22210 0.64 +1.0 4,631,795 - +0.1 4,631,928 - +0.3 4,632,049 - -0.5 4,632,331 + BWI76_RS22215 0.37 +0.6 4,632,390 + BWI76_RS22215 0.47 +0.4 4,632,427 - BWI76_RS22215 0.54 +0.9 4,632,560 + BWI76_RS22215 0.77 +0.2 4,632,994 - BWI76_RS22220 0.32 +0.9 4,633,055 + BWI76_RS22220 0.38 +1.1 4,633,335 - BWI76_RS22220 0.67 +1.0 4,633,346 - BWI76_RS22220 0.68 -2.5 4,633,415 + BWI76_RS22220 0.75 -0.7 4,633,551 + BWI76_RS22220 0.89 +0.1 4,633,559 - BWI76_RS22220 0.89 -0.3 4,633,559 - BWI76_RS22220 0.89 +0.3
Or see this region's nucleotide sequence