Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22065 and BWI76_RS22070 overlap by 8 nucleotides BWI76_RS22070 and BWI76_RS22075 are separated by 62 nucleotides
BWI76_RS22065: BWI76_RS22065 - glycine betaine/L-proline ABC transporter ATP-binding protein, at 4,606,430 to 4,607,632
_RS22065
BWI76_RS22070: BWI76_RS22070 - proline/betaine ABC transporter permease ProW, at 4,607,625 to 4,608,689
_RS22070
BWI76_RS22075: BWI76_RS22075 - glycine betaine ABC transporter substrate-binding protein, at 4,608,752 to 4,609,747
_RS22075
Position (kb) 4607
4608
4609 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4606.668 kb on - strand, within BWI76_RS22065 at 4606.681 kb on + strand, within BWI76_RS22065 at 4606.770 kb on + strand, within BWI76_RS22065 at 4606.786 kb on + strand, within BWI76_RS22065 at 4606.794 kb on - strand, within BWI76_RS22065 at 4606.794 kb on - strand, within BWI76_RS22065 at 4606.794 kb on - strand, within BWI76_RS22065 at 4606.823 kb on + strand, within BWI76_RS22065 at 4606.823 kb on + strand, within BWI76_RS22065 at 4606.826 kb on - strand, within BWI76_RS22065 at 4606.826 kb on - strand, within BWI76_RS22065 at 4606.831 kb on - strand, within BWI76_RS22065 at 4606.863 kb on - strand, within BWI76_RS22065 at 4606.876 kb on + strand, within BWI76_RS22065 at 4606.906 kb on + strand, within BWI76_RS22065 at 4606.914 kb on - strand, within BWI76_RS22065 at 4606.922 kb on + strand, within BWI76_RS22065 at 4606.924 kb on + strand, within BWI76_RS22065 at 4606.924 kb on + strand, within BWI76_RS22065 at 4606.932 kb on - strand, within BWI76_RS22065 at 4606.932 kb on - strand, within BWI76_RS22065 at 4606.932 kb on - strand, within BWI76_RS22065 at 4606.932 kb on - strand, within BWI76_RS22065 at 4606.940 kb on - strand, within BWI76_RS22065 at 4606.944 kb on + strand, within BWI76_RS22065 at 4606.945 kb on + strand, within BWI76_RS22065 at 4606.949 kb on + strand, within BWI76_RS22065 at 4606.949 kb on + strand, within BWI76_RS22065 at 4606.949 kb on + strand, within BWI76_RS22065 at 4606.949 kb on + strand, within BWI76_RS22065 at 4606.949 kb on + strand, within BWI76_RS22065 at 4606.949 kb on + strand, within BWI76_RS22065 at 4606.952 kb on - strand, within BWI76_RS22065 at 4606.952 kb on - strand, within BWI76_RS22065 at 4606.952 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand, within BWI76_RS22065 at 4606.957 kb on - strand at 4606.985 kb on - strand, within BWI76_RS22065 at 4606.994 kb on + strand, within BWI76_RS22065 at 4606.994 kb on + strand, within BWI76_RS22065 at 4607.002 kb on - strand, within BWI76_RS22065 at 4607.007 kb on - strand, within BWI76_RS22065 at 4607.017 kb on - strand, within BWI76_RS22065 at 4607.017 kb on - strand, within BWI76_RS22065 at 4607.027 kb on - strand, within BWI76_RS22065 at 4607.100 kb on + strand, within BWI76_RS22065 at 4607.104 kb on + strand, within BWI76_RS22065 at 4607.108 kb on - strand, within BWI76_RS22065 at 4607.112 kb on - strand, within BWI76_RS22065 at 4607.119 kb on - strand, within BWI76_RS22065 at 4607.148 kb on + strand, within BWI76_RS22065 at 4607.148 kb on + strand, within BWI76_RS22065 at 4607.159 kb on + strand, within BWI76_RS22065 at 4607.159 kb on + strand, within BWI76_RS22065 at 4607.167 kb on - strand, within BWI76_RS22065 at 4607.172 kb on - strand, within BWI76_RS22065 at 4607.240 kb on + strand, within BWI76_RS22065 at 4607.240 kb on + strand, within BWI76_RS22065 at 4607.256 kb on - strand, within BWI76_RS22065 at 4607.269 kb on - strand, within BWI76_RS22065 at 4607.277 kb on + strand, within BWI76_RS22065 at 4607.280 kb on + strand, within BWI76_RS22065 at 4607.285 kb on + strand, within BWI76_RS22065 at 4607.315 kb on - strand, within BWI76_RS22065 at 4607.369 kb on + strand, within BWI76_RS22065 at 4607.394 kb on + strand, within BWI76_RS22065 at 4607.418 kb on + strand, within BWI76_RS22065 at 4607.428 kb on + strand, within BWI76_RS22065 at 4607.428 kb on + strand, within BWI76_RS22065 at 4607.428 kb on + strand, within BWI76_RS22065 at 4607.428 kb on + strand, within BWI76_RS22065 at 4607.428 kb on + strand, within BWI76_RS22065 at 4607.428 kb on + strand, within BWI76_RS22065 at 4607.431 kb on - strand, within BWI76_RS22065 at 4607.436 kb on - strand, within BWI76_RS22065 at 4607.436 kb on - strand, within BWI76_RS22065 at 4607.436 kb on - strand, within BWI76_RS22065 at 4607.453 kb on + strand, within BWI76_RS22065 at 4607.453 kb on + strand, within BWI76_RS22065 at 4607.461 kb on - strand, within BWI76_RS22065 at 4607.461 kb on - strand, within BWI76_RS22065 at 4607.461 kb on - strand, within BWI76_RS22065 at 4607.461 kb on - strand, within BWI76_RS22065 at 4607.466 kb on + strand, within BWI76_RS22065 at 4607.473 kb on + strand, within BWI76_RS22065 at 4607.481 kb on - strand, within BWI76_RS22065 at 4607.509 kb on + strand, within BWI76_RS22065 at 4607.515 kb on + strand at 4607.520 kb on + strand at 4607.520 kb on + strand at 4607.528 kb on - strand at 4607.528 kb on - strand at 4607.601 kb on - strand at 4607.601 kb on - strand at 4607.621 kb on + strand at 4607.653 kb on - strand at 4607.696 kb on + strand at 4607.701 kb on + strand at 4607.709 kb on - strand at 4607.709 kb on - strand at 4607.710 kb on - strand at 4607.904 kb on - strand, within BWI76_RS22070 at 4608.001 kb on - strand, within BWI76_RS22070 at 4608.183 kb on + strand, within BWI76_RS22070 at 4608.196 kb on - strand, within BWI76_RS22070 at 4608.220 kb on + strand, within BWI76_RS22070 at 4608.388 kb on - strand, within BWI76_RS22070 at 4608.655 kb on + strand at 4608.840 kb on + strand at 4608.843 kb on - strand at 4608.916 kb on + strand, within BWI76_RS22075 at 4608.968 kb on - strand, within BWI76_RS22075 at 4609.027 kb on + strand, within BWI76_RS22075 at 4609.129 kb on + strand, within BWI76_RS22075 at 4609.229 kb on + strand, within BWI76_RS22075 at 4609.287 kb on - strand, within BWI76_RS22075 at 4609.439 kb on + strand, within BWI76_RS22075 at 4609.458 kb on - strand, within BWI76_RS22075 at 4609.538 kb on + strand, within BWI76_RS22075 at 4609.551 kb on - strand, within BWI76_RS22075
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,606,668 - BWI76_RS22065 0.20 -0.5 4,606,681 + BWI76_RS22065 0.21 +0.3 4,606,770 + BWI76_RS22065 0.28 -0.1 4,606,786 + BWI76_RS22065 0.30 +0.8 4,606,794 - BWI76_RS22065 0.30 +0.1 4,606,794 - BWI76_RS22065 0.30 -0.7 4,606,794 - BWI76_RS22065 0.30 +1.0 4,606,823 + BWI76_RS22065 0.33 +0.0 4,606,823 + BWI76_RS22065 0.33 -0.1 4,606,826 - BWI76_RS22065 0.33 +0.7 4,606,826 - BWI76_RS22065 0.33 -2.0 4,606,831 - BWI76_RS22065 0.33 -1.0 4,606,863 - BWI76_RS22065 0.36 +0.2 4,606,876 + BWI76_RS22065 0.37 -0.1 4,606,906 + BWI76_RS22065 0.40 -0.1 4,606,914 - BWI76_RS22065 0.40 +0.4 4,606,922 + BWI76_RS22065 0.41 -0.1 4,606,924 + BWI76_RS22065 0.41 +1.0 4,606,924 + BWI76_RS22065 0.41 -0.0 4,606,932 - BWI76_RS22065 0.42 -0.1 4,606,932 - BWI76_RS22065 0.42 +0.7 4,606,932 - BWI76_RS22065 0.42 -0.1 4,606,932 - BWI76_RS22065 0.42 +0.7 4,606,940 - BWI76_RS22065 0.42 -1.5 4,606,944 + BWI76_RS22065 0.43 -0.6 4,606,945 + BWI76_RS22065 0.43 +0.9 4,606,949 + BWI76_RS22065 0.43 +0.2 4,606,949 + BWI76_RS22065 0.43 -3.1 4,606,949 + BWI76_RS22065 0.43 -0.2 4,606,949 + BWI76_RS22065 0.43 -1.3 4,606,949 + BWI76_RS22065 0.43 -1.1 4,606,949 + BWI76_RS22065 0.43 -0.3 4,606,952 - BWI76_RS22065 0.43 -0.4 4,606,952 - BWI76_RS22065 0.43 -0.2 4,606,952 - BWI76_RS22065 0.43 -0.5 4,606,957 - BWI76_RS22065 0.44 +1.3 4,606,957 - BWI76_RS22065 0.44 +0.0 4,606,957 - BWI76_RS22065 0.44 -0.1 4,606,957 - BWI76_RS22065 0.44 -0.5 4,606,957 - BWI76_RS22065 0.44 +0.5 4,606,957 - BWI76_RS22065 0.44 +0.6 4,606,957 - BWI76_RS22065 0.44 -2.3 4,606,957 - -0.5 4,606,985 - BWI76_RS22065 0.46 +0.9 4,606,994 + BWI76_RS22065 0.47 -0.4 4,606,994 + BWI76_RS22065 0.47 +0.5 4,607,002 - BWI76_RS22065 0.48 -0.1 4,607,007 - BWI76_RS22065 0.48 -0.5 4,607,017 - BWI76_RS22065 0.49 -0.0 4,607,017 - BWI76_RS22065 0.49 -1.5 4,607,027 - BWI76_RS22065 0.50 -0.5 4,607,100 + BWI76_RS22065 0.56 +0.5 4,607,104 + BWI76_RS22065 0.56 +0.6 4,607,108 - BWI76_RS22065 0.56 +0.5 4,607,112 - BWI76_RS22065 0.57 +0.6 4,607,119 - BWI76_RS22065 0.57 +0.2 4,607,148 + BWI76_RS22065 0.60 +0.4 4,607,148 + BWI76_RS22065 0.60 +0.0 4,607,159 + BWI76_RS22065 0.61 -1.6 4,607,159 + BWI76_RS22065 0.61 -0.5 4,607,167 - BWI76_RS22065 0.61 -0.6 4,607,172 - BWI76_RS22065 0.62 +0.5 4,607,240 + BWI76_RS22065 0.67 -1.6 4,607,240 + BWI76_RS22065 0.67 -1.3 4,607,256 - BWI76_RS22065 0.69 +1.7 4,607,269 - BWI76_RS22065 0.70 -0.5 4,607,277 + BWI76_RS22065 0.70 +1.0 4,607,280 + BWI76_RS22065 0.71 -1.3 4,607,285 + BWI76_RS22065 0.71 +1.6 4,607,315 - BWI76_RS22065 0.74 -1.3 4,607,369 + BWI76_RS22065 0.78 +0.4 4,607,394 + BWI76_RS22065 0.80 +0.1 4,607,418 + BWI76_RS22065 0.82 -1.5 4,607,428 + BWI76_RS22065 0.83 -0.5 4,607,428 + BWI76_RS22065 0.83 -1.0 4,607,428 + BWI76_RS22065 0.83 +0.2 4,607,428 + BWI76_RS22065 0.83 -1.0 4,607,428 + BWI76_RS22065 0.83 -0.6 4,607,428 + BWI76_RS22065 0.83 -0.7 4,607,431 - BWI76_RS22065 0.83 +0.4 4,607,436 - BWI76_RS22065 0.84 +1.0 4,607,436 - BWI76_RS22065 0.84 +1.0 4,607,436 - BWI76_RS22065 0.84 -1.2 4,607,453 + BWI76_RS22065 0.85 -2.1 4,607,453 + BWI76_RS22065 0.85 +0.7 4,607,461 - BWI76_RS22065 0.86 -1.5 4,607,461 - BWI76_RS22065 0.86 -0.1 4,607,461 - BWI76_RS22065 0.86 +0.5 4,607,461 - BWI76_RS22065 0.86 +1.0 4,607,466 + BWI76_RS22065 0.86 +1.6 4,607,473 + BWI76_RS22065 0.87 -0.0 4,607,481 - BWI76_RS22065 0.87 -0.3 4,607,509 + BWI76_RS22065 0.90 -0.3 4,607,515 + +0.5 4,607,520 + +0.8 4,607,520 + -1.0 4,607,528 - +0.5 4,607,528 - +0.4 4,607,601 - -0.4 4,607,601 - -0.8 4,607,621 + +0.3 4,607,653 - -0.6 4,607,696 + -0.4 4,607,701 + -0.0 4,607,709 - -0.5 4,607,709 - +0.4 4,607,710 - -0.4 4,607,904 - BWI76_RS22070 0.26 -1.4 4,608,001 - BWI76_RS22070 0.35 -1.2 4,608,183 + BWI76_RS22070 0.52 +1.0 4,608,196 - BWI76_RS22070 0.54 -1.3 4,608,220 + BWI76_RS22070 0.56 -1.7 4,608,388 - BWI76_RS22070 0.72 -1.3 4,608,655 + +2.0 4,608,840 + -2.0 4,608,843 - +1.9 4,608,916 + BWI76_RS22075 0.16 -2.4 4,608,968 - BWI76_RS22075 0.22 +0.3 4,609,027 + BWI76_RS22075 0.28 -0.2 4,609,129 + BWI76_RS22075 0.38 -0.6 4,609,229 + BWI76_RS22075 0.48 -0.1 4,609,287 - BWI76_RS22075 0.54 -0.2 4,609,439 + BWI76_RS22075 0.69 +1.0 4,609,458 - BWI76_RS22075 0.71 -1.0 4,609,538 + BWI76_RS22075 0.79 -1.0 4,609,551 - BWI76_RS22075 0.80 +0.5
Or see this region's nucleotide sequence