Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21560 and BWI76_RS21565 are separated by 147 nucleotides BWI76_RS21565 and BWI76_RS21570 are separated by 65 nucleotides BWI76_RS21570 and BWI76_RS21575 overlap by 11 nucleotides BWI76_RS21575 and BWI76_RS21580 are separated by 110 nucleotides
BWI76_RS21560: BWI76_RS21560 - DNA repair protein RecN, at 4,513,052 to 4,514,713
_RS21560
BWI76_RS21565: BWI76_RS21565 - outer membrane protein assembly factor BamE, at 4,514,861 to 4,515,202
_RS21565
BWI76_RS21570: BWI76_RS21570 - RnfH family protein, at 4,515,268 to 4,515,558
_RS21570
BWI76_RS21575: BWI76_RS21575 - hypothetical protein, at 4,515,548 to 4,516,024
_RS21575
BWI76_RS21580: BWI76_RS21580 - SsrA-binding protein, at 4,516,135 to 4,516,617
_RS21580
Position (kb) 4515
4516 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4514.506 kb on + strand, within BWI76_RS21560 at 4514.549 kb on - strand at 4514.600 kb on + strand at 4514.822 kb on + strand at 4515.166 kb on - strand, within BWI76_RS21565 at 4515.280 kb on + strand at 4515.361 kb on - strand at 4515.402 kb on + strand, within BWI76_RS21570 at 4515.489 kb on + strand, within BWI76_RS21570 at 4515.558 kb on + strand at 4515.566 kb on - strand at 4515.566 kb on - strand at 4516.070 kb on - strand at 4516.131 kb on - strand at 4516.288 kb on + strand, within BWI76_RS21580 at 4516.402 kb on + strand, within BWI76_RS21580 at 4516.469 kb on - strand, within BWI76_RS21580
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,514,506 + BWI76_RS21560 0.87 -2.0 4,514,549 - -1.8 4,514,600 + -0.6 4,514,822 + -0.4 4,515,166 - BWI76_RS21565 0.89 -0.3 4,515,280 + -0.5 4,515,361 - +0.0 4,515,402 + BWI76_RS21570 0.46 -3.4 4,515,489 + BWI76_RS21570 0.76 -1.7 4,515,558 + +1.0 4,515,566 - +0.0 4,515,566 - +0.4 4,516,070 - -0.6 4,516,131 - -2.4 4,516,288 + BWI76_RS21580 0.32 +1.0 4,516,402 + BWI76_RS21580 0.55 -1.8 4,516,469 - BWI76_RS21580 0.69 +0.0
Or see this region's nucleotide sequence