Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20315 and BWI76_RS20320 are separated by 41 nucleotides BWI76_RS20320 and BWI76_RS20325 are separated by 7 nucleotides BWI76_RS20325 and BWI76_RS20330 overlap by 4 nucleotides BWI76_RS20330 and BWI76_RS20335 are separated by 66 nucleotides
BWI76_RS20315: BWI76_RS20315 - N-acetyltransferase, at 4,254,233 to 4,254,730
_RS20315
BWI76_RS20320: BWI76_RS20320 - histidine/lysine/arginine/ornithine ABC transporter ATP-binding protein HisP, at 4,254,772 to 4,255,545
_RS20320
BWI76_RS20325: BWI76_RS20325 - histidine/lysine/arginine/ornithine ABC transporter permease HisM, at 4,255,553 to 4,256,269
_RS20325
BWI76_RS20330: BWI76_RS20330 - histidine/lysine/arginine/ornithine ABC transporter permease HisQ, at 4,256,266 to 4,256,952
_RS20330
BWI76_RS20335: BWI76_RS20335 - zinc transporter, at 4,257,019 to 4,257,801
_RS20335
Position (kb) 4255
4256
4257 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4254.561 kb on + strand, within BWI76_RS20315 at 4254.777 kb on - strand at 4255.231 kb on + strand, within BWI76_RS20320 at 4255.312 kb on - strand, within BWI76_RS20320 at 4255.478 kb on + strand at 4255.486 kb on - strand at 4255.487 kb on + strand at 4255.505 kb on + strand at 4255.507 kb on - strand at 4255.824 kb on - strand, within BWI76_RS20325 at 4255.893 kb on - strand, within BWI76_RS20325 at 4256.054 kb on + strand, within BWI76_RS20325 at 4256.074 kb on + strand, within BWI76_RS20325 at 4256.271 kb on + strand at 4256.302 kb on - strand at 4256.322 kb on - strand at 4256.360 kb on + strand, within BWI76_RS20330 at 4256.425 kb on - strand, within BWI76_RS20330 at 4256.449 kb on + strand, within BWI76_RS20330 at 4256.722 kb on + strand, within BWI76_RS20330 at 4256.802 kb on - strand, within BWI76_RS20330 at 4256.898 kb on + strand at 4256.898 kb on + strand at 4256.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,254,561 + BWI76_RS20315 0.66 -0.3 4,254,777 - -0.6 4,255,231 + BWI76_RS20320 0.59 -0.1 4,255,312 - BWI76_RS20320 0.70 +0.1 4,255,478 + +1.3 4,255,486 - -0.3 4,255,487 + -1.2 4,255,505 + +0.4 4,255,507 - +2.1 4,255,824 - BWI76_RS20325 0.38 +0.3 4,255,893 - BWI76_RS20325 0.47 +0.5 4,256,054 + BWI76_RS20325 0.70 +0.8 4,256,074 + BWI76_RS20325 0.73 +0.6 4,256,271 + +0.5 4,256,302 - -1.0 4,256,322 - -2.3 4,256,360 + BWI76_RS20330 0.14 +0.4 4,256,425 - BWI76_RS20330 0.23 +0.6 4,256,449 + BWI76_RS20330 0.27 -1.1 4,256,722 + BWI76_RS20330 0.66 +0.9 4,256,802 - BWI76_RS20330 0.78 +0.1 4,256,898 + +1.5 4,256,898 + -4.5 4,256,906 - -0.6
Or see this region's nucleotide sequence