Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20110 and BWI76_RS20115 are separated by 30 nucleotides BWI76_RS20115 and BWI76_RS20120 are separated by 62 nucleotides
BWI76_RS20110: BWI76_RS20110 - fimbrial assembly protein, at 4,211,975 to 4,214,524
_RS20110
BWI76_RS20115: BWI76_RS20115 - hypothetical protein, at 4,214,555 to 4,215,217
_RS20115
BWI76_RS20120: BWI76_RS20120 - fimbrial protein, at 4,215,280 to 4,215,807
_RS20120
Position (kb)
4214
4215
4216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4213.669 kb on + strand, within BWI76_RS20110 at 4213.828 kb on - strand, within BWI76_RS20110 at 4214.046 kb on + strand, within BWI76_RS20110 at 4214.046 kb on + strand, within BWI76_RS20110 at 4214.046 kb on + strand, within BWI76_RS20110 at 4214.157 kb on - strand, within BWI76_RS20110 at 4214.334 kb on + strand at 4214.445 kb on + strand at 4214.453 kb on - strand at 4214.536 kb on - strand at 4214.545 kb on - strand at 4214.550 kb on - strand at 4214.573 kb on - strand at 4214.628 kb on - strand, within BWI76_RS20115 at 4214.880 kb on + strand, within BWI76_RS20115 at 4214.880 kb on + strand, within BWI76_RS20115 at 4214.888 kb on - strand at 4215.066 kb on - strand, within BWI76_RS20115 at 4215.070 kb on + strand, within BWI76_RS20115 at 4215.402 kb on + strand, within BWI76_RS20120 at 4215.414 kb on - strand, within BWI76_RS20120 at 4215.479 kb on + strand, within BWI76_RS20120 at 4215.487 kb on - strand, within BWI76_RS20120 at 4215.668 kb on + strand, within BWI76_RS20120 at 4215.676 kb on - strand, within BWI76_RS20120 at 4215.730 kb on - strand, within BWI76_RS20120 at 4215.730 kb on - strand, within BWI76_RS20120 at 4215.734 kb on + strand, within BWI76_RS20120 at 4215.745 kb on - strand, within BWI76_RS20120 at 4215.745 kb on - strand, within BWI76_RS20120 at 4215.745 kb on - strand, within BWI76_RS20120 at 4215.747 kb on + strand, within BWI76_RS20120 at 4215.747 kb on + strand, within BWI76_RS20120 at 4215.747 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.772 kb on + strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.788 kb on - strand at 4215.818 kb on + strand at 4215.818 kb on + strand at 4215.826 kb on - strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.885 kb on + strand at 4215.952 kb on + strand at 4216.028 kb on - strand at 4216.073 kb on - strand at 4216.074 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,213,669 + BWI76_RS20110 0.66 +0.3 4,213,828 - BWI76_RS20110 0.73 -0.2 4,214,046 + BWI76_RS20110 0.81 -1.1 4,214,046 + BWI76_RS20110 0.81 -2.5 4,214,046 + BWI76_RS20110 0.81 -2.6 4,214,157 - BWI76_RS20110 0.86 -0.1 4,214,334 + +0.0 4,214,445 + -2.6 4,214,453 - -0.7 4,214,536 - -1.2 4,214,545 - -0.4 4,214,550 - -0.2 4,214,573 - -0.6 4,214,628 - BWI76_RS20115 0.11 +1.6 4,214,880 + BWI76_RS20115 0.49 -0.7 4,214,880 + BWI76_RS20115 0.49 -0.7 4,214,888 - +2.1 4,215,066 - BWI76_RS20115 0.77 +1.0 4,215,070 + BWI76_RS20115 0.78 +0.4 4,215,402 + BWI76_RS20120 0.23 +0.2 4,215,414 - BWI76_RS20120 0.25 -0.4 4,215,479 + BWI76_RS20120 0.38 +0.5 4,215,487 - BWI76_RS20120 0.39 +0.7 4,215,668 + BWI76_RS20120 0.73 -0.3 4,215,676 - BWI76_RS20120 0.75 -2.6 4,215,730 - BWI76_RS20120 0.85 -0.2 4,215,730 - BWI76_RS20120 0.85 -2.3 4,215,734 + BWI76_RS20120 0.86 +0.3 4,215,745 - BWI76_RS20120 0.88 +0.4 4,215,745 - BWI76_RS20120 0.88 -2.4 4,215,745 - BWI76_RS20120 0.88 +1.2 4,215,747 + BWI76_RS20120 0.88 +1.3 4,215,747 + BWI76_RS20120 0.88 -0.3 4,215,747 + BWI76_RS20120 0.88 +0.9 4,215,752 + BWI76_RS20120 0.89 -0.1 4,215,752 + BWI76_RS20120 0.89 +0.8 4,215,752 + BWI76_RS20120 0.89 +0.8 4,215,752 + BWI76_RS20120 0.89 -1.9 4,215,752 + BWI76_RS20120 0.89 +0.9 4,215,752 + BWI76_RS20120 0.89 +0.6 4,215,752 + BWI76_RS20120 0.89 -1.1 4,215,760 - +0.4 4,215,760 - +1.1 4,215,760 - +0.4 4,215,760 - -3.1 4,215,760 - -1.4 4,215,760 - +0.3 4,215,760 - -0.4 4,215,760 - -1.7 4,215,760 - +1.1 4,215,760 - -0.6 4,215,772 + -0.6 4,215,780 - +1.0 4,215,780 - -0.3 4,215,780 - -1.1 4,215,780 - -0.4 4,215,780 - +0.9 4,215,780 - +0.8 4,215,780 - -0.7 4,215,780 - +0.3 4,215,788 - -0.2 4,215,818 + +0.7 4,215,818 + -1.0 4,215,826 - -1.9 4,215,848 + -0.2 4,215,848 + -0.4 4,215,848 + -0.3 4,215,848 + -1.1 4,215,848 + +0.4 4,215,856 - +0.0 4,215,856 - -0.2 4,215,856 - -0.1 4,215,856 - +0.8 4,215,856 - +2.1 4,215,856 - +0.2 4,215,856 - +0.2 4,215,885 + +0.9 4,215,952 + -0.4 4,216,028 - -0.3 4,216,073 - -1.1 4,216,074 - +0.9
Or see this region's nucleotide sequence