Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19525 and BWI76_RS19530 are separated by 174 nucleotides BWI76_RS19530 and BWI76_RS19535 are separated by 155 nucleotides BWI76_RS19535 and BWI76_RS19540 are separated by 105 nucleotides
BWI76_RS19525: BWI76_RS19525 - D-alanyl-D-alanine endopeptidase, at 4,081,298 to 4,082,245
_RS19525
BWI76_RS19530: BWI76_RS19530 - YIP1 family protein, at 4,082,420 to 4,083,007
_RS19530
BWI76_RS19535: BWI76_RS19535 - putative integral membrane protein, at 4,083,163 to 4,083,732
_RS19535
BWI76_RS19540: BWI76_RS19540 - multidrug transporter permease, at 4,083,838 to 4,085,280
_RS19540
Position (kb) 4082
4083
4084 Strain fitness (log2 ratio) -1
0
1
2
3 at 4081.612 kb on + strand, within BWI76_RS19525 at 4082.036 kb on - strand, within BWI76_RS19525 at 4082.209 kb on + strand at 4082.217 kb on - strand at 4082.217 kb on - strand at 4082.307 kb on - strand at 4082.309 kb on - strand at 4082.309 kb on - strand at 4082.426 kb on + strand at 4082.455 kb on - strand at 4082.510 kb on + strand, within BWI76_RS19530 at 4082.580 kb on + strand, within BWI76_RS19530 at 4082.580 kb on + strand, within BWI76_RS19530 at 4082.801 kb on - strand, within BWI76_RS19530 at 4082.925 kb on - strand, within BWI76_RS19530 at 4083.031 kb on + strand at 4083.039 kb on - strand at 4083.120 kb on + strand at 4083.254 kb on - strand, within BWI76_RS19535 at 4083.273 kb on - strand, within BWI76_RS19535 at 4083.432 kb on - strand, within BWI76_RS19535 at 4083.767 kb on - strand at 4083.860 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,081,612 + BWI76_RS19525 0.33 +0.5 4,082,036 - BWI76_RS19525 0.78 -0.9 4,082,209 + +0.1 4,082,217 - -0.0 4,082,217 - -1.1 4,082,307 - -0.3 4,082,309 - +1.8 4,082,309 - -0.1 4,082,426 + +0.8 4,082,455 - -1.0 4,082,510 + BWI76_RS19530 0.15 +0.2 4,082,580 + BWI76_RS19530 0.27 +1.3 4,082,580 + BWI76_RS19530 0.27 +0.3 4,082,801 - BWI76_RS19530 0.65 +0.1 4,082,925 - BWI76_RS19530 0.86 +2.9 4,083,031 + +0.8 4,083,039 - +0.1 4,083,120 + -0.5 4,083,254 - BWI76_RS19535 0.16 +1.3 4,083,273 - BWI76_RS19535 0.19 -1.0 4,083,432 - BWI76_RS19535 0.47 +0.1 4,083,767 - +0.5 4,083,860 + +0.1
Or see this region's nucleotide sequence