Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18000

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17995 and BWI76_RS18000 are separated by 36 nucleotidesBWI76_RS18000 and BWI76_RS18005 are separated by 115 nucleotidesBWI76_RS18005 and BWI76_RS18010 are separated by 97 nucleotidesBWI76_RS18010 and BWI76_RS18015 overlap by 4 nucleotides BWI76_RS17995: BWI76_RS17995 - hypothetical protein, at 3,738,772 to 3,741,405 _RS17995 BWI76_RS18000: BWI76_RS18000 - 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF, at 3,741,442 to 3,742,917 _RS18000 BWI76_RS18005: BWI76_RS18005 - hypothetical protein, at 3,743,033 to 3,743,272 _RS18005 BWI76_RS18010: BWI76_RS18010 - hypothetical protein, at 3,743,370 to 3,743,561 _RS18010 BWI76_RS18015: BWI76_RS18015 - serine/threonine-protein phosphatase, at 3,743,558 to 3,744,211 _RS18015 Position (kb) 3741 3742 3743Strain fitness (log2 ratio) -2 -1 0 1at 3740.719 kb on + strand, within BWI76_RS17995at 3740.760 kb on - strand, within BWI76_RS17995at 3740.816 kb on + strandat 3740.954 kb on - strand, within BWI76_RS17995at 3741.265 kb on - strandat 3741.700 kb on - strand, within BWI76_RS18000at 3742.331 kb on - strand, within BWI76_RS18000at 3742.387 kb on + strand, within BWI76_RS18000at 3742.530 kb on + strand, within BWI76_RS18000at 3742.744 kb on + strand, within BWI76_RS18000at 3742.801 kb on + strandat 3743.072 kb on + strand, within BWI76_RS18005at 3743.084 kb on - strand, within BWI76_RS18005at 3743.084 kb on - strand, within BWI76_RS18005at 3743.096 kb on + strand, within BWI76_RS18005at 3743.201 kb on + strand, within BWI76_RS18005at 3743.336 kb on - strandat 3743.336 kb on - strandat 3743.385 kb on - strandat 3743.659 kb on + strand, within BWI76_RS18015at 3743.766 kb on - strand, within BWI76_RS18015at 3743.819 kb on + strand, within BWI76_RS18015at 3743.829 kb on - strand, within BWI76_RS18015

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
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3,740,719 + BWI76_RS17995 0.74 -0.3
3,740,760 - BWI76_RS17995 0.75 +1.2
3,740,816 + -0.2
3,740,954 - BWI76_RS17995 0.83 -2.2
3,741,265 - +1.4
3,741,700 - BWI76_RS18000 0.17 -0.5
3,742,331 - BWI76_RS18000 0.60 -1.6
3,742,387 + BWI76_RS18000 0.64 +1.4
3,742,530 + BWI76_RS18000 0.74 -2.2
3,742,744 + BWI76_RS18000 0.88 -0.9
3,742,801 + -0.3
3,743,072 + BWI76_RS18005 0.16 +1.4
3,743,084 - BWI76_RS18005 0.21 +0.4
3,743,084 - BWI76_RS18005 0.21 +0.3
3,743,096 + BWI76_RS18005 0.26 +0.6
3,743,201 + BWI76_RS18005 0.70 +1.1
3,743,336 - -0.9
3,743,336 - -1.9
3,743,385 - +1.4
3,743,659 + BWI76_RS18015 0.15 -1.7
3,743,766 - BWI76_RS18015 0.32 -0.0
3,743,819 + BWI76_RS18015 0.40 +0.8
3,743,829 - BWI76_RS18015 0.41 -1.4

Or see this region's nucleotide sequence