Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16465 and BWI76_RS16470 are separated by 142 nucleotides BWI76_RS16470 and BWI76_RS16475 are separated by 53 nucleotides BWI76_RS16475 and BWI76_RS16480 are separated by 79 nucleotides BWI76_RS16480 and BWI76_RS16485 are separated by 106 nucleotides
BWI76_RS16465: BWI76_RS16465 - DUF1289 domain-containing protein, at 3,415,061 to 3,415,300
_RS16465
BWI76_RS16470: BWI76_RS16470 - transcriptional repressor NemR, at 3,415,443 to 3,416,042
_RS16470
BWI76_RS16475: BWI76_RS16475 - alkene reductase, at 3,416,096 to 3,417,193
_RS16475
BWI76_RS16480: BWI76_RS16480 - lactoylglutathione lyase, at 3,417,273 to 3,417,680
_RS16480
BWI76_RS16485: BWI76_RS16485 - ribonuclease T, at 3,417,787 to 3,418,431
_RS16485
Position (kb) 3416
3417
3418 Strain fitness (log2 ratio) -1
0
1
2 at 3415.448 kb on + strand at 3415.499 kb on + strand at 3415.619 kb on + strand, within BWI76_RS16470 at 3416.082 kb on + strand at 3416.187 kb on + strand at 3416.195 kb on - strand at 3416.213 kb on - strand, within BWI76_RS16475 at 3416.290 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.325 kb on + strand, within BWI76_RS16475 at 3416.333 kb on - strand, within BWI76_RS16475 at 3416.717 kb on + strand, within BWI76_RS16475 at 3416.725 kb on - strand, within BWI76_RS16475 at 3417.036 kb on + strand, within BWI76_RS16475 at 3417.229 kb on + strand at 3417.237 kb on - strand at 3417.334 kb on + strand, within BWI76_RS16480 at 3417.476 kb on + strand, within BWI76_RS16480 at 3417.527 kb on - strand, within BWI76_RS16480 at 3417.658 kb on + strand at 3417.804 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 3,415,448 + -1.1 3,415,499 + +0.5 3,415,619 + BWI76_RS16470 0.29 -0.8 3,416,082 + +0.0 3,416,187 + -0.4 3,416,195 - +0.0 3,416,213 - BWI76_RS16475 0.11 +0.5 3,416,290 + BWI76_RS16475 0.18 +0.3 3,416,325 + BWI76_RS16475 0.21 +0.5 3,416,325 + BWI76_RS16475 0.21 +1.0 3,416,333 - BWI76_RS16475 0.22 +0.6 3,416,717 + BWI76_RS16475 0.57 +1.8 3,416,725 - BWI76_RS16475 0.57 +1.8 3,417,036 + BWI76_RS16475 0.86 -1.0 3,417,229 + -0.5 3,417,237 - -1.4 3,417,334 + BWI76_RS16480 0.15 +1.2 3,417,476 + BWI76_RS16480 0.50 +0.0 3,417,527 - BWI76_RS16480 0.62 -1.3 3,417,658 + -0.2 3,417,804 + -0.6
Or see this region's nucleotide sequence