Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12180 and BWI76_RS12185 overlap by 11 nucleotides BWI76_RS12185 and BWI76_RS12190 are separated by 7 nucleotides BWI76_RS12190 and BWI76_RS12195 are separated by 2 nucleotides BWI76_RS12195 and BWI76_RS12200 overlap by 50 nucleotides
BWI76_RS12180: BWI76_RS12180 - membrane protein, at 2,552,135 to 2,552,524
_RS12180
BWI76_RS12185: BWI76_RS12185 - hypothetical protein, at 2,552,514 to 2,552,792
_RS12185
BWI76_RS12190: BWI76_RS12190 - glycoside hydrolase family protein, at 2,552,800 to 2,553,429
_RS12190
BWI76_RS12195: BWI76_RS12195 - hypothetical protein, at 2,553,432 to 2,553,707
_RS12195
BWI76_RS12200: BWI76_RS12200 - hypothetical protein, at 2,553,658 to 2,553,852
_RS12200
Position (kb)
2552
2553 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2551.516 kb on - strand at 2551.520 kb on + strand at 2551.530 kb on + strand at 2551.548 kb on + strand at 2551.741 kb on + strand at 2551.791 kb on - strand at 2551.806 kb on + strand at 2551.855 kb on + strand at 2551.855 kb on + strand at 2551.855 kb on + strand at 2551.884 kb on - strand at 2551.930 kb on - strand at 2551.951 kb on + strand at 2552.025 kb on - strand at 2552.116 kb on + strand at 2552.118 kb on + strand at 2552.119 kb on - strand at 2552.124 kb on - strand at 2552.179 kb on + strand, within BWI76_RS12180 at 2552.179 kb on + strand, within BWI76_RS12180 at 2552.187 kb on - strand, within BWI76_RS12180 at 2552.337 kb on + strand, within BWI76_RS12180 at 2552.337 kb on + strand, within BWI76_RS12180 at 2552.342 kb on + strand, within BWI76_RS12180 at 2552.342 kb on + strand, within BWI76_RS12180 at 2552.342 kb on + strand, within BWI76_RS12180 at 2552.360 kb on - strand, within BWI76_RS12180 at 2552.370 kb on + strand, within BWI76_RS12180 at 2552.375 kb on + strand, within BWI76_RS12180 at 2552.502 kb on - strand at 2552.596 kb on + strand, within BWI76_RS12185 at 2552.606 kb on - strand, within BWI76_RS12185 at 2552.653 kb on - strand, within BWI76_RS12185 at 2553.015 kb on + strand at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.033 kb on + strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.036 kb on - strand, within BWI76_RS12190 at 2553.045 kb on + strand at 2553.047 kb on + strand, within BWI76_RS12190 at 2553.054 kb on + strand at 2553.081 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.140 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.160 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.287 kb on - strand, within BWI76_RS12190 at 2553.289 kb on + strand, within BWI76_RS12190 at 2553.400 kb on - strand at 2553.496 kb on + strand, within BWI76_RS12195 at 2553.504 kb on - strand, within BWI76_RS12195 at 2553.553 kb on - strand, within BWI76_RS12195 at 2553.578 kb on - strand, within BWI76_RS12195 at 2553.615 kb on - strand at 2553.627 kb on - strand, within BWI76_RS12195 at 2553.719 kb on + strand, within BWI76_RS12200 at 2553.751 kb on - strand, within BWI76_RS12200
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 2,551,516 - +0.0 2,551,520 + -0.4 2,551,530 + -0.8 2,551,548 + -0.1 2,551,741 + -0.5 2,551,791 - -0.2 2,551,806 + -0.2 2,551,855 + -0.4 2,551,855 + -2.2 2,551,855 + -0.8 2,551,884 - -0.8 2,551,930 - +0.0 2,551,951 + +0.6 2,552,025 - -1.6 2,552,116 + -0.1 2,552,118 + -0.4 2,552,119 - -0.3 2,552,124 - +0.5 2,552,179 + BWI76_RS12180 0.11 -0.1 2,552,179 + BWI76_RS12180 0.11 -3.9 2,552,187 - BWI76_RS12180 0.13 -1.3 2,552,337 + BWI76_RS12180 0.52 -1.6 2,552,337 + BWI76_RS12180 0.52 -0.9 2,552,342 + BWI76_RS12180 0.53 -1.4 2,552,342 + BWI76_RS12180 0.53 -0.7 2,552,342 + BWI76_RS12180 0.53 -2.9 2,552,360 - BWI76_RS12180 0.58 -0.3 2,552,370 + BWI76_RS12180 0.60 -0.5 2,552,375 + BWI76_RS12180 0.62 -0.5 2,552,502 - +1.0 2,552,596 + BWI76_RS12185 0.29 -1.4 2,552,606 - BWI76_RS12185 0.33 -0.6 2,552,653 - BWI76_RS12185 0.50 +1.0 2,553,015 + -0.3 2,553,033 + BWI76_RS12190 0.37 -0.9 2,553,033 + BWI76_RS12190 0.37 -0.3 2,553,036 - BWI76_RS12190 0.37 -1.0 2,553,036 - BWI76_RS12190 0.37 -3.6 2,553,045 + +1.0 2,553,047 + BWI76_RS12190 0.39 -0.4 2,553,054 + -1.3 2,553,081 - BWI76_RS12190 0.45 -2.4 2,553,140 - BWI76_RS12190 0.54 -0.7 2,553,140 - BWI76_RS12190 0.54 -0.5 2,553,160 - BWI76_RS12190 0.57 -0.3 2,553,160 - BWI76_RS12190 0.57 +0.2 2,553,287 - BWI76_RS12190 0.77 -0.2 2,553,287 - BWI76_RS12190 0.77 -1.8 2,553,289 + BWI76_RS12190 0.78 -0.7 2,553,400 - -0.4 2,553,496 + BWI76_RS12195 0.23 -1.0 2,553,504 - BWI76_RS12195 0.26 -0.6 2,553,553 - BWI76_RS12195 0.44 -4.2 2,553,578 - BWI76_RS12195 0.53 +0.0 2,553,615 - +1.0 2,553,627 - BWI76_RS12195 0.71 +0.1 2,553,719 + BWI76_RS12200 0.31 -1.1 2,553,751 - BWI76_RS12200 0.48 -0.6
Or see this region's nucleotide sequence