Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09325 and BWI76_RS09330 are separated by 68 nucleotides BWI76_RS09330 and BWI76_RS09335 overlap by 4 nucleotides BWI76_RS09335 and BWI76_RS09340 overlap by 14 nucleotides BWI76_RS09340 and BWI76_RS09345 overlap by 11 nucleotides
BWI76_RS09325: BWI76_RS09325 - putative bacteriophage tail completion protein, at 1,958,420 to 1,958,866
_RS09325
BWI76_RS09330: BWI76_RS09330 - baseplate assembly protein V, at 1,958,935 to 1,959,507
_RS09330
BWI76_RS09335: BWI76_RS09335 - baseplate assembly protein W, at 1,959,504 to 1,959,866
_RS09335
BWI76_RS09340: BWI76_RS09340 - baseplate assembly protein J, at 1,959,853 to 1,960,761
_RS09340
BWI76_RS09345: BWI76_RS09345 - phage tail protein I, at 1,960,751 to 1,961,359
_RS09345
Position (kb) 1959
1960 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1958.602 kb on + strand, within BWI76_RS09325 at 1958.769 kb on + strand, within BWI76_RS09325 at 1959.352 kb on + strand, within BWI76_RS09330 at 1959.433 kb on + strand, within BWI76_RS09330 at 1959.775 kb on + strand, within BWI76_RS09335 at 1959.901 kb on - strand at 1960.048 kb on - strand, within BWI76_RS09340 at 1960.156 kb on + strand, within BWI76_RS09340 at 1960.366 kb on + strand, within BWI76_RS09340 at 1960.416 kb on + strand, within BWI76_RS09340 at 1960.429 kb on - strand, within BWI76_RS09340 at 1960.434 kb on - strand, within BWI76_RS09340 at 1960.554 kb on - strand, within BWI76_RS09340 at 1960.618 kb on - strand, within BWI76_RS09340 at 1960.701 kb on - strand at 1960.815 kb on - strand, within BWI76_RS09345
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,958,602 + BWI76_RS09325 0.41 -0.1 1,958,769 + BWI76_RS09325 0.78 -0.2 1,959,352 + BWI76_RS09330 0.73 -0.2 1,959,433 + BWI76_RS09330 0.87 -3.0 1,959,775 + BWI76_RS09335 0.75 -2.7 1,959,901 - +0.1 1,960,048 - BWI76_RS09340 0.21 -1.0 1,960,156 + BWI76_RS09340 0.33 -0.1 1,960,366 + BWI76_RS09340 0.56 +0.3 1,960,416 + BWI76_RS09340 0.62 +0.5 1,960,429 - BWI76_RS09340 0.63 +0.5 1,960,434 - BWI76_RS09340 0.64 -0.4 1,960,554 - BWI76_RS09340 0.77 +0.5 1,960,618 - BWI76_RS09340 0.84 +0.3 1,960,701 - -0.2 1,960,815 - BWI76_RS09345 0.11 +0.5
Or see this region's nucleotide sequence