Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09065

Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09060 and BWI76_RS09065 are separated by 136 nucleotidesBWI76_RS09065 and BWI76_RS09070 are separated by 209 nucleotides BWI76_RS09060: BWI76_RS09060 - hypothetical protein, at 1,911,179 to 1,912,444 _RS09060 BWI76_RS09065: BWI76_RS09065 - sugar phosphatase SupH, at 1,912,581 to 1,913,396 _RS09065 BWI76_RS09070: BWI76_RS09070 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 1,913,606 to 1,916,038 _RS09070 Position (kb) 1912 1913 1914Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1911.667 kb on - strand, within BWI76_RS09060at 1911.710 kb on - strand, within BWI76_RS09060at 1912.334 kb on + strandat 1912.435 kb on - strandat 1912.570 kb on - strandat 1912.791 kb on - strand, within BWI76_RS09065at 1912.817 kb on - strand, within BWI76_RS09065at 1912.827 kb on - strand, within BWI76_RS09065at 1913.000 kb on + strand, within BWI76_RS09065at 1913.593 kb on - strandat 1913.657 kb on - strandat 1913.713 kb on + strandat 1914.238 kb on - strand, within BWI76_RS09070at 1914.290 kb on - strand, within BWI76_RS09070at 1914.308 kb on - strand, within BWI76_RS09070at 1914.388 kb on + strand, within BWI76_RS09070at 1914.388 kb on + strand, within BWI76_RS09070at 1914.396 kb on - strand, within BWI76_RS09070at 1914.396 kb on - strand, within BWI76_RS09070

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
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1,911,667 - BWI76_RS09060 0.39 -0.7
1,911,710 - BWI76_RS09060 0.42 +0.2
1,912,334 + -0.5
1,912,435 - -0.3
1,912,570 - +0.5
1,912,791 - BWI76_RS09065 0.26 +0.1
1,912,817 - BWI76_RS09065 0.29 +0.2
1,912,827 - BWI76_RS09065 0.30 +1.5
1,913,000 + BWI76_RS09065 0.51 +1.8
1,913,593 - -0.1
1,913,657 - +0.0
1,913,713 + -0.1
1,914,238 - BWI76_RS09070 0.26 -0.8
1,914,290 - BWI76_RS09070 0.28 +0.3
1,914,308 - BWI76_RS09070 0.29 -2.8
1,914,388 + BWI76_RS09070 0.32 -0.0
1,914,388 + BWI76_RS09070 0.32 -1.6
1,914,396 - BWI76_RS09070 0.32 +0.0
1,914,396 - BWI76_RS09070 0.32 +0.9

Or see this region's nucleotide sequence