Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06110 and BWI76_RS06115 overlap by 4 nucleotides BWI76_RS06115 and BWI76_RS06120 are separated by 255 nucleotides BWI76_RS06120 and BWI76_RS06125 are separated by 151 nucleotides BWI76_RS06125 and BWI76_RS06130 are separated by 101 nucleotides BWI76_RS06130 and BWI76_RS06135 are separated by 169 nucleotides
BWI76_RS06110: BWI76_RS06110 - hypothetical protein, at 1,284,849 to 1,285,955
_RS06110
BWI76_RS06115: BWI76_RS06115 - membrane protein, at 1,285,952 to 1,286,254
_RS06115
BWI76_RS06120: BWI76_RS06120 - membrane protein, at 1,286,510 to 1,286,713
_RS06120
BWI76_RS06125: BWI76_RS06125 - D-alanine--D-alanine ligase A, at 1,286,865 to 1,287,962
_RS06125
BWI76_RS06130: BWI76_RS06130 - extensin family protein, at 1,288,064 to 1,288,747
_RS06130
BWI76_RS06135: BWI76_RS06135 - MFS transporter, at 1,288,917 to 1,290,116
_RS06135
Position (kb) 1286
1287
1288 Strain fitness (log2 ratio) -1
0
1
2 at 1285.944 kb on - strand at 1286.548 kb on - strand, within BWI76_RS06120 at 1286.553 kb on - strand, within BWI76_RS06120 at 1286.766 kb on + strand at 1286.965 kb on + strand at 1286.972 kb on + strand at 1287.054 kb on - strand, within BWI76_RS06125 at 1287.253 kb on + strand, within BWI76_RS06125 at 1287.364 kb on + strand, within BWI76_RS06125 at 1287.477 kb on + strand, within BWI76_RS06125 at 1287.490 kb on - strand, within BWI76_RS06125 at 1287.506 kb on + strand, within BWI76_RS06125 at 1287.657 kb on + strand, within BWI76_RS06125 at 1287.705 kb on + strand, within BWI76_RS06125 at 1287.760 kb on - strand, within BWI76_RS06125 at 1287.766 kb on - strand, within BWI76_RS06125 at 1287.990 kb on + strand at 1288.076 kb on + strand at 1288.122 kb on + strand at 1288.615 kb on + strand, within BWI76_RS06130 at 1288.623 kb on - strand, within BWI76_RS06130 at 1288.768 kb on - strand at 1288.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,285,944 - -0.4 1,286,548 - BWI76_RS06120 0.19 -0.3 1,286,553 - BWI76_RS06120 0.21 -1.7 1,286,766 + -0.6 1,286,965 + +0.1 1,286,972 + +1.0 1,287,054 - BWI76_RS06125 0.17 +0.5 1,287,253 + BWI76_RS06125 0.35 +1.1 1,287,364 + BWI76_RS06125 0.45 +1.0 1,287,477 + BWI76_RS06125 0.56 +1.8 1,287,490 - BWI76_RS06125 0.57 +0.7 1,287,506 + BWI76_RS06125 0.58 -0.0 1,287,657 + BWI76_RS06125 0.72 +1.0 1,287,705 + BWI76_RS06125 0.77 +0.5 1,287,760 - BWI76_RS06125 0.82 +1.0 1,287,766 - BWI76_RS06125 0.82 +0.6 1,287,990 + +0.4 1,288,076 + -0.5 1,288,122 + -1.6 1,288,615 + BWI76_RS06130 0.81 +0.5 1,288,623 - BWI76_RS06130 0.82 +0.3 1,288,768 - +0.5 1,288,768 - -0.4
Or see this region's nucleotide sequence