Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05550 and BWI76_RS05555 are separated by 57 nucleotides BWI76_RS05555 and BWI76_RS05560 are separated by 76 nucleotides BWI76_RS05560 and BWI76_RS05565 are separated by 2 nucleotides
BWI76_RS05550: BWI76_RS05550 - fermentation/respiration switch protein, at 1,162,885 to 1,164,129
_RS05550
BWI76_RS05555: BWI76_RS05555 - Crl family RNA polymerase assembly factor, at 1,164,187 to 1,164,585
_RS05555
BWI76_RS05560: BWI76_RS05560 - serine acetyltransferase, at 1,164,662 to 1,165,168
_RS05560
BWI76_RS05565: BWI76_RS05565 - beta-galactosidase, at 1,165,171 to 1,166,589
_RS05565
Position (kb) 1164
1165 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1163.257 kb on - strand, within BWI76_RS05550 at 1163.370 kb on + strand, within BWI76_RS05550 at 1163.542 kb on - strand, within BWI76_RS05550 at 1163.577 kb on - strand, within BWI76_RS05550 at 1163.607 kb on + strand, within BWI76_RS05550 at 1163.768 kb on + strand, within BWI76_RS05550 at 1163.776 kb on - strand, within BWI76_RS05550 at 1163.776 kb on - strand, within BWI76_RS05550 at 1163.928 kb on - strand, within BWI76_RS05550 at 1163.958 kb on + strand, within BWI76_RS05550 at 1164.077 kb on - strand at 1164.121 kb on - strand at 1164.167 kb on - strand at 1164.198 kb on - strand at 1164.207 kb on + strand at 1164.207 kb on + strand at 1164.215 kb on - strand at 1164.296 kb on + strand, within BWI76_RS05555 at 1164.396 kb on - strand, within BWI76_RS05555 at 1165.323 kb on - strand, within BWI76_RS05565 at 1165.330 kb on + strand, within BWI76_RS05565 at 1165.348 kb on - strand, within BWI76_RS05565 at 1165.348 kb on - strand, within BWI76_RS05565 at 1165.383 kb on + strand, within BWI76_RS05565
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,163,257 - BWI76_RS05550 0.30 -1.6 1,163,370 + BWI76_RS05550 0.39 +0.2 1,163,542 - BWI76_RS05550 0.53 +0.9 1,163,577 - BWI76_RS05550 0.56 +0.7 1,163,607 + BWI76_RS05550 0.58 +0.0 1,163,768 + BWI76_RS05550 0.71 -1.3 1,163,776 - BWI76_RS05550 0.72 -1.7 1,163,776 - BWI76_RS05550 0.72 -0.6 1,163,928 - BWI76_RS05550 0.84 +0.4 1,163,958 + BWI76_RS05550 0.86 +2.3 1,164,077 - +0.4 1,164,121 - -0.5 1,164,167 - +0.0 1,164,198 - -1.7 1,164,207 + -1.3 1,164,207 + -1.8 1,164,215 - -1.5 1,164,296 + BWI76_RS05555 0.27 -1.5 1,164,396 - BWI76_RS05555 0.52 -3.5 1,165,323 - BWI76_RS05565 0.11 -0.5 1,165,330 + BWI76_RS05565 0.11 -0.2 1,165,348 - BWI76_RS05565 0.12 +0.0 1,165,348 - BWI76_RS05565 0.12 +0.1 1,165,383 + BWI76_RS05565 0.15 -0.8
Or see this region's nucleotide sequence