Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05405 and BWI76_RS05410 are separated by 163 nucleotides BWI76_RS05410 and BWI76_RS05415 are separated by 30 nucleotides BWI76_RS05415 and BWI76_RS05420 are separated by 104 nucleotides
BWI76_RS05405: BWI76_RS05405 - tRNA-Asp, at 1,135,931 to 1,136,007
_RS05405
BWI76_RS05410: BWI76_RS05410 - 2,5-didehydrogluconate reductase B, at 1,136,171 to 1,136,974
_RS05410
BWI76_RS05415: BWI76_RS05415 - LysR family transcriptional regulator, at 1,137,005 to 1,137,907
_RS05415
BWI76_RS05420: BWI76_RS05420 - MFS transporter, at 1,138,012 to 1,139,202
_RS05420
Position (kb) 1137
1138 Strain fitness (log2 ratio) -1
0
1
2
3 at 1136.583 kb on - strand, within BWI76_RS05410 at 1136.637 kb on + strand, within BWI76_RS05410 at 1136.675 kb on + strand, within BWI76_RS05410 at 1136.785 kb on + strand, within BWI76_RS05410 at 1137.065 kb on - strand at 1137.349 kb on + strand, within BWI76_RS05415 at 1137.383 kb on - strand, within BWI76_RS05415 at 1137.448 kb on + strand, within BWI76_RS05415 at 1137.448 kb on + strand at 1137.456 kb on - strand, within BWI76_RS05415 at 1137.456 kb on - strand, within BWI76_RS05415 at 1137.471 kb on - strand, within BWI76_RS05415 at 1138.097 kb on + strand at 1138.250 kb on - strand, within BWI76_RS05420 at 1138.258 kb on - strand, within BWI76_RS05420 at 1138.364 kb on + strand, within BWI76_RS05420 at 1138.421 kb on + strand, within BWI76_RS05420 at 1138.507 kb on - strand, within BWI76_RS05420 at 1138.532 kb on + strand, within BWI76_RS05420 at 1138.540 kb on - strand, within BWI76_RS05420 at 1138.597 kb on + strand, within BWI76_RS05420 at 1138.631 kb on + strand, within BWI76_RS05420 at 1138.714 kb on - strand, within BWI76_RS05420
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,136,583 - BWI76_RS05410 0.51 +0.7 1,136,637 + BWI76_RS05410 0.58 -0.1 1,136,675 + BWI76_RS05410 0.63 +0.1 1,136,785 + BWI76_RS05410 0.76 -0.6 1,137,065 - +0.1 1,137,349 + BWI76_RS05415 0.38 +0.3 1,137,383 - BWI76_RS05415 0.42 -0.1 1,137,448 + BWI76_RS05415 0.49 +0.8 1,137,448 + -1.0 1,137,456 - BWI76_RS05415 0.50 +0.2 1,137,456 - BWI76_RS05415 0.50 +2.4 1,137,471 - BWI76_RS05415 0.52 +0.3 1,138,097 + -0.2 1,138,250 - BWI76_RS05420 0.20 +0.1 1,138,258 - BWI76_RS05420 0.21 -0.2 1,138,364 + BWI76_RS05420 0.30 -0.3 1,138,421 + BWI76_RS05420 0.34 +0.1 1,138,507 - BWI76_RS05420 0.42 -0.7 1,138,532 + BWI76_RS05420 0.44 +0.3 1,138,540 - BWI76_RS05420 0.44 -1.1 1,138,597 + BWI76_RS05420 0.49 +3.0 1,138,631 + BWI76_RS05420 0.52 -1.1 1,138,714 - BWI76_RS05420 0.59 +1.1
Or see this region's nucleotide sequence