Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05185 and BWI76_RS05190 are separated by 46 nucleotides BWI76_RS05190 and BWI76_RS05195 are separated by 107 nucleotides BWI76_RS05195 and BWI76_RS05200 are separated by 34 nucleotides
BWI76_RS05185: BWI76_RS05185 - CdaR family transcriptional regulator, at 1,091,228 to 1,092,385
_RS05185
BWI76_RS05190: BWI76_RS05190 - hypothetical protein, at 1,092,432 to 1,092,821
_RS05190
BWI76_RS05195: BWI76_RS05195 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,092,929 to 1,093,753
_RS05195
BWI76_RS05200: BWI76_RS05200 - bifunctional uridylyltransferase/uridylyl-removing protein, at 1,093,788 to 1,096,451
_RS05200
Position (kb)
1092
1093 Strain fitness (log2 ratio)
-2
-1
0
1 at 1091.457 kb on + strand, within BWI76_RS05185 at 1091.548 kb on + strand, within BWI76_RS05185 at 1091.616 kb on - strand, within BWI76_RS05185 at 1091.627 kb on + strand, within BWI76_RS05185 at 1091.662 kb on - strand, within BWI76_RS05185 at 1091.803 kb on + strand, within BWI76_RS05185 at 1091.806 kb on - strand, within BWI76_RS05185 at 1091.806 kb on - strand, within BWI76_RS05185 at 1092.204 kb on + strand, within BWI76_RS05185 at 1092.268 kb on + strand, within BWI76_RS05185 at 1092.308 kb on - strand at 1092.344 kb on + strand at 1092.354 kb on + strand at 1092.357 kb on - strand at 1092.357 kb on - strand at 1092.374 kb on - strand at 1092.599 kb on - strand, within BWI76_RS05190 at 1092.599 kb on - strand, within BWI76_RS05190 at 1092.604 kb on - strand, within BWI76_RS05190 at 1092.627 kb on + strand, within BWI76_RS05190 at 1092.627 kb on + strand, within BWI76_RS05190 at 1092.627 kb on + strand, within BWI76_RS05190 at 1092.627 kb on - strand at 1092.635 kb on - strand, within BWI76_RS05190 at 1092.635 kb on - strand, within BWI76_RS05190 at 1092.635 kb on - strand, within BWI76_RS05190 at 1092.706 kb on + strand, within BWI76_RS05190 at 1092.706 kb on + strand, within BWI76_RS05190 at 1092.714 kb on - strand, within BWI76_RS05190 at 1092.755 kb on + strand, within BWI76_RS05190 at 1092.784 kb on + strand at 1092.789 kb on + strand at 1092.794 kb on + strand at 1092.802 kb on - strand at 1092.818 kb on + strand at 1092.837 kb on - strand at 1092.927 kb on + strand at 1093.792 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 1,091,457 + BWI76_RS05185 0.20 +0.3 1,091,548 + BWI76_RS05185 0.28 +0.0 1,091,616 - BWI76_RS05185 0.34 +0.3 1,091,627 + BWI76_RS05185 0.34 -0.4 1,091,662 - BWI76_RS05185 0.37 +0.0 1,091,803 + BWI76_RS05185 0.50 -0.1 1,091,806 - BWI76_RS05185 0.50 -0.0 1,091,806 - BWI76_RS05185 0.50 -1.0 1,092,204 + BWI76_RS05185 0.84 +0.9 1,092,268 + BWI76_RS05185 0.90 -0.1 1,092,308 - +0.3 1,092,344 + -0.5 1,092,354 + +0.6 1,092,357 - -2.2 1,092,357 - -0.8 1,092,374 - -0.2 1,092,599 - BWI76_RS05190 0.43 -1.1 1,092,599 - BWI76_RS05190 0.43 -0.6 1,092,604 - BWI76_RS05190 0.44 +0.5 1,092,627 + BWI76_RS05190 0.50 +0.2 1,092,627 + BWI76_RS05190 0.50 -1.3 1,092,627 + BWI76_RS05190 0.50 -1.5 1,092,627 - +1.0 1,092,635 - BWI76_RS05190 0.52 -1.9 1,092,635 - BWI76_RS05190 0.52 -0.3 1,092,635 - BWI76_RS05190 0.52 -1.1 1,092,706 + BWI76_RS05190 0.70 -2.1 1,092,706 + BWI76_RS05190 0.70 -0.2 1,092,714 - BWI76_RS05190 0.72 -1.7 1,092,755 + BWI76_RS05190 0.83 -0.2 1,092,784 + -2.0 1,092,789 + -0.3 1,092,794 + +0.5 1,092,802 - +1.0 1,092,818 + +1.0 1,092,837 - -1.7 1,092,927 + -2.2 1,093,792 + -0.6
Or see this region's nucleotide sequence