Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04150 and BWI76_RS04155 are separated by 10 nucleotides BWI76_RS04155 and BWI76_RS04160 are separated by 104 nucleotides BWI76_RS04160 and BWI76_RS04165 are separated by 9 nucleotides
BWI76_RS04150: BWI76_RS04150 - lipoate--protein ligase A, at 853,899 to 854,915
_RS04150
BWI76_RS04155: BWI76_RS04155 - membrane protein, at 854,926 to 855,567
_RS04155
BWI76_RS04160: BWI76_RS04160 - phosphoserine phosphatase, at 855,672 to 856,643
_RS04160
BWI76_RS04165: BWI76_RS04165 - DNA repair protein RadA, at 856,653 to 858,035
_RS04165
Position (kb) 855
856
857 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 854.972 kb on - strand at 855.070 kb on + strand, within BWI76_RS04155 at 855.251 kb on - strand, within BWI76_RS04155 at 855.251 kb on - strand, within BWI76_RS04155 at 855.486 kb on + strand, within BWI76_RS04155 at 855.504 kb on - strand at 855.512 kb on - strand at 855.522 kb on - strand at 855.691 kb on + strand at 855.726 kb on + strand at 855.797 kb on + strand, within BWI76_RS04160 at 855.866 kb on - strand, within BWI76_RS04160 at 855.919 kb on + strand, within BWI76_RS04160 at 855.927 kb on - strand, within BWI76_RS04160 at 856.200 kb on - strand, within BWI76_RS04160 at 856.310 kb on + strand, within BWI76_RS04160 at 856.331 kb on - strand, within BWI76_RS04160 at 856.571 kb on + strand at 856.593 kb on + strand at 856.598 kb on + strand at 856.603 kb on - strand at 856.606 kb on - strand at 856.617 kb on + strand at 856.695 kb on + strand at 857.087 kb on - strand, within BWI76_RS04165 at 857.376 kb on - strand, within BWI76_RS04165 at 857.473 kb on + strand, within BWI76_RS04165
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 854,972 - +0.1 855,070 + BWI76_RS04155 0.22 +0.5 855,251 - BWI76_RS04155 0.51 +1.2 855,251 - BWI76_RS04155 0.51 +0.2 855,486 + BWI76_RS04155 0.87 -0.1 855,504 - +1.7 855,512 - +0.8 855,522 - -0.1 855,691 + +0.8 855,726 + +0.3 855,797 + BWI76_RS04160 0.13 -0.5 855,866 - BWI76_RS04160 0.20 +0.5 855,919 + BWI76_RS04160 0.25 -0.4 855,927 - BWI76_RS04160 0.26 -2.9 856,200 - BWI76_RS04160 0.54 -1.5 856,310 + BWI76_RS04160 0.66 -2.9 856,331 - BWI76_RS04160 0.68 +1.0 856,571 + -2.3 856,593 + +0.3 856,598 + +1.0 856,603 - -1.0 856,606 - -0.5 856,617 + +0.5 856,695 + -0.6 857,087 - BWI76_RS04165 0.31 +0.4 857,376 - BWI76_RS04165 0.52 -0.2 857,473 + BWI76_RS04165 0.59 -1.3
Or see this region's nucleotide sequence