Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03900 and BWI76_RS03905 overlap by 4 nucleotides BWI76_RS03905 and BWI76_RS03910 overlap by 14 nucleotides BWI76_RS03910 and BWI76_RS03915 overlap by 4 nucleotides BWI76_RS03915 and BWI76_RS03920 are separated by 70 nucleotides
BWI76_RS03900: BWI76_RS03900 - Na+/proline symporter, at 806,232 to 807,719
_RS03900
BWI76_RS03905: BWI76_RS03905 - 2-oxo-3-deoxygalactonate kinase, at 807,716 to 808,588
_RS03905
BWI76_RS03910: BWI76_RS03910 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 808,575 to 809,177
_RS03910
BWI76_RS03915: BWI76_RS03915 - MurR/RpiR family transcriptional regulator, at 809,174 to 810,037
_RS03915
BWI76_RS03920: BWI76_RS03920 - N-acetyltransferase, at 810,108 to 810,641
_RS03920
Position (kb)
808
809
810 Strain fitness (log2 ratio)
-1
0
1
2
3 at 807.698 kb on + strand at 807.706 kb on - strand at 807.747 kb on + strand at 807.755 kb on - strand at 807.859 kb on + strand, within BWI76_RS03905 at 807.951 kb on - strand, within BWI76_RS03905 at 807.983 kb on - strand, within BWI76_RS03905 at 808.000 kb on + strand, within BWI76_RS03905 at 808.008 kb on - strand, within BWI76_RS03905 at 808.114 kb on + strand, within BWI76_RS03905 at 808.122 kb on - strand at 808.146 kb on - strand, within BWI76_RS03905 at 808.314 kb on - strand, within BWI76_RS03905 at 808.451 kb on - strand, within BWI76_RS03905 at 808.503 kb on - strand at 808.582 kb on + strand at 808.774 kb on - strand, within BWI76_RS03910 at 808.779 kb on - strand, within BWI76_RS03910 at 809.235 kb on - strand at 809.333 kb on - strand at 809.380 kb on - strand, within BWI76_RS03915 at 809.384 kb on - strand, within BWI76_RS03915 at 809.484 kb on + strand, within BWI76_RS03915 at 809.656 kb on - strand, within BWI76_RS03915 at 809.718 kb on + strand, within BWI76_RS03915 at 809.768 kb on - strand, within BWI76_RS03915 at 809.998 kb on + strand at 810.120 kb on + strand at 810.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 807,698 + +0.8 807,706 - +0.7 807,747 + +0.2 807,755 - +0.8 807,859 + BWI76_RS03905 0.16 -0.1 807,951 - BWI76_RS03905 0.27 +0.3 807,983 - BWI76_RS03905 0.31 +1.1 808,000 + BWI76_RS03905 0.33 -0.1 808,008 - BWI76_RS03905 0.33 +0.5 808,114 + BWI76_RS03905 0.46 +2.5 808,122 - +1.1 808,146 - BWI76_RS03905 0.49 -1.7 808,314 - BWI76_RS03905 0.68 -0.8 808,451 - BWI76_RS03905 0.84 -0.2 808,503 - -0.3 808,582 + -0.4 808,774 - BWI76_RS03910 0.33 -0.6 808,779 - BWI76_RS03910 0.34 -1.1 809,235 - -0.3 809,333 - +3.4 809,380 - BWI76_RS03915 0.24 -0.9 809,384 - BWI76_RS03915 0.24 +0.4 809,484 + BWI76_RS03915 0.36 +0.0 809,656 - BWI76_RS03915 0.56 +0.4 809,718 + BWI76_RS03915 0.63 -0.3 809,768 - BWI76_RS03915 0.69 +0.4 809,998 + -0.1 810,120 + +1.6 810,158 - +0.7
Or see this region's nucleotide sequence