Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02765 and BWI76_RS02770 are separated by 185 nucleotides BWI76_RS02770 and BWI76_RS02775 are separated by 209 nucleotides
BWI76_RS02765: BWI76_RS02765 - membrane protein, at 560,837 to 562,180
_RS02765
BWI76_RS02770: BWI76_RS02770 - peptide-methionine (S)-S-oxide reductase, at 562,366 to 563,004
_RS02770
BWI76_RS02775: BWI76_RS02775 - outer membrane protein assembly factor, at 563,214 to 564,947
_RS02775
Position (kb)
562
563
564 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 561.427 kb on + strand, within BWI76_RS02765 at 561.493 kb on - strand, within BWI76_RS02765 at 561.691 kb on + strand, within BWI76_RS02765 at 561.795 kb on + strand, within BWI76_RS02765 at 561.878 kb on + strand, within BWI76_RS02765 at 561.914 kb on + strand, within BWI76_RS02765 at 561.922 kb on + strand, within BWI76_RS02765 at 562.013 kb on - strand at 562.050 kb on + strand at 562.050 kb on + strand at 562.050 kb on + strand at 562.096 kb on - strand at 562.284 kb on + strand at 562.284 kb on + strand at 562.292 kb on - strand at 562.292 kb on - strand at 562.539 kb on + strand, within BWI76_RS02770 at 562.572 kb on - strand, within BWI76_RS02770 at 562.572 kb on - strand, within BWI76_RS02770 at 562.636 kb on - strand, within BWI76_RS02770 at 562.636 kb on - strand, within BWI76_RS02770 at 562.672 kb on - strand, within BWI76_RS02770 at 562.685 kb on - strand, within BWI76_RS02770 at 562.741 kb on + strand, within BWI76_RS02770 at 562.741 kb on + strand, within BWI76_RS02770 at 562.747 kb on - strand, within BWI76_RS02770 at 562.799 kb on + strand, within BWI76_RS02770 at 562.833 kb on - strand, within BWI76_RS02770 at 562.850 kb on - strand, within BWI76_RS02770 at 562.916 kb on + strand, within BWI76_RS02770 at 562.926 kb on - strand, within BWI76_RS02770 at 562.998 kb on + strand at 563.012 kb on - strand at 563.038 kb on + strand at 563.132 kb on + strand at 563.156 kb on + strand at 563.186 kb on - strand at 563.579 kb on - strand, within BWI76_RS02775 at 563.782 kb on + strand, within BWI76_RS02775 at 563.797 kb on + strand, within BWI76_RS02775
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 561,427 + BWI76_RS02765 0.44 +0.0 561,493 - BWI76_RS02765 0.49 -1.1 561,691 + BWI76_RS02765 0.64 +0.2 561,795 + BWI76_RS02765 0.71 -0.1 561,878 + BWI76_RS02765 0.77 -0.4 561,914 + BWI76_RS02765 0.80 +0.3 561,922 + BWI76_RS02765 0.81 +1.2 562,013 - +1.2 562,050 + +0.3 562,050 + -1.0 562,050 + +0.8 562,096 - +0.2 562,284 + +0.2 562,284 + -0.5 562,292 - +0.3 562,292 - +0.5 562,539 + BWI76_RS02770 0.27 +0.9 562,572 - BWI76_RS02770 0.32 +0.7 562,572 - BWI76_RS02770 0.32 +0.5 562,636 - BWI76_RS02770 0.42 +1.0 562,636 - BWI76_RS02770 0.42 +0.4 562,672 - BWI76_RS02770 0.48 +0.5 562,685 - BWI76_RS02770 0.50 +0.2 562,741 + BWI76_RS02770 0.59 -0.0 562,741 + BWI76_RS02770 0.59 +0.4 562,747 - BWI76_RS02770 0.60 +0.1 562,799 + BWI76_RS02770 0.68 +1.0 562,833 - BWI76_RS02770 0.73 +0.5 562,850 - BWI76_RS02770 0.76 -0.5 562,916 + BWI76_RS02770 0.86 +0.6 562,926 - BWI76_RS02770 0.88 -1.4 562,998 + +0.6 563,012 - +0.4 563,038 + -0.4 563,132 + +0.7 563,156 + +0.1 563,186 - -0.8 563,579 - BWI76_RS02775 0.21 -1.6 563,782 + BWI76_RS02775 0.33 -3.5 563,797 + BWI76_RS02775 0.34 -1.8
Or see this region's nucleotide sequence