Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02305 and BWI76_RS02310 are separated by 67 nucleotides BWI76_RS02310 and BWI76_RS02315 are separated by 35 nucleotides
BWI76_RS02305: BWI76_RS02305 - hypothetical protein, at 473,077 to 475,164
_RS02305
BWI76_RS02310: BWI76_RS02310 - transcriptional regulator, at 475,232 to 475,807
_RS02310
BWI76_RS02315: BWI76_RS02315 - protein-disulfide reductase DsbD, at 475,843 to 477,600
_RS02315
Position (kb)
475
476 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 474.351 kb on + strand, within BWI76_RS02305 at 474.390 kb on + strand, within BWI76_RS02305 at 474.390 kb on + strand, within BWI76_RS02305 at 474.395 kb on - strand, within BWI76_RS02305 at 474.400 kb on - strand, within BWI76_RS02305 at 474.523 kb on + strand, within BWI76_RS02305 at 474.664 kb on + strand, within BWI76_RS02305 at 474.672 kb on - strand, within BWI76_RS02305 at 474.890 kb on + strand, within BWI76_RS02305 at 474.996 kb on - strand at 475.014 kb on + strand at 475.121 kb on + strand at 475.127 kb on - strand at 475.146 kb on + strand at 475.172 kb on - strand at 475.172 kb on - strand at 475.251 kb on + strand at 475.251 kb on + strand at 475.328 kb on - strand, within BWI76_RS02310 at 475.346 kb on + strand, within BWI76_RS02310 at 475.540 kb on + strand, within BWI76_RS02310 at 475.548 kb on - strand, within BWI76_RS02310 at 475.592 kb on - strand, within BWI76_RS02310 at 475.688 kb on - strand, within BWI76_RS02310 at 475.699 kb on + strand, within BWI76_RS02310 at 475.854 kb on - strand at 475.906 kb on - strand at 475.973 kb on - strand at 476.037 kb on + strand, within BWI76_RS02315 at 476.045 kb on - strand, within BWI76_RS02315 at 476.078 kb on - strand, within BWI76_RS02315 at 476.263 kb on - strand, within BWI76_RS02315 at 476.270 kb on + strand, within BWI76_RS02315 at 476.492 kb on + strand, within BWI76_RS02315 at 476.586 kb on + strand, within BWI76_RS02315 at 476.686 kb on + strand, within BWI76_RS02315 at 476.694 kb on - strand, within BWI76_RS02315 at 476.786 kb on - strand, within BWI76_RS02315
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 474,351 + BWI76_RS02305 0.61 -1.2 474,390 + BWI76_RS02305 0.63 -0.4 474,390 + BWI76_RS02305 0.63 -0.8 474,395 - BWI76_RS02305 0.63 +1.2 474,400 - BWI76_RS02305 0.63 +0.3 474,523 + BWI76_RS02305 0.69 +0.7 474,664 + BWI76_RS02305 0.76 -0.1 474,672 - BWI76_RS02305 0.76 +0.6 474,890 + BWI76_RS02305 0.87 -0.1 474,996 - +0.6 475,014 + +2.0 475,121 + +1.2 475,127 - +0.7 475,146 + +1.4 475,172 - +0.7 475,172 - -0.9 475,251 + +0.5 475,251 + -1.8 475,328 - BWI76_RS02310 0.17 +1.2 475,346 + BWI76_RS02310 0.20 -1.4 475,540 + BWI76_RS02310 0.53 -0.7 475,548 - BWI76_RS02310 0.55 -0.8 475,592 - BWI76_RS02310 0.62 -0.5 475,688 - BWI76_RS02310 0.79 -0.8 475,699 + BWI76_RS02310 0.81 -0.5 475,854 - -1.4 475,906 - +0.2 475,973 - -0.9 476,037 + BWI76_RS02315 0.11 -2.4 476,045 - BWI76_RS02315 0.11 -0.3 476,078 - BWI76_RS02315 0.13 -0.6 476,263 - BWI76_RS02315 0.24 +0.7 476,270 + BWI76_RS02315 0.24 +0.2 476,492 + BWI76_RS02315 0.37 -0.8 476,586 + BWI76_RS02315 0.42 -1.0 476,686 + BWI76_RS02315 0.48 -0.7 476,694 - BWI76_RS02315 0.48 -1.4 476,786 - BWI76_RS02315 0.54 +0.9
Or see this region's nucleotide sequence