Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01145 and BWI76_RS01150 are separated by 55 nucleotides BWI76_RS01150 and BWI76_RS01155 are separated by 22 nucleotides BWI76_RS01155 and BWI76_RS01160 are separated by 45 nucleotides BWI76_RS01160 and BWI76_RS01165 are separated by 461 nucleotides BWI76_RS01165 and BWI76_RS01170 are separated by 3 nucleotides
BWI76_RS01145: BWI76_RS01145 - tRNA-Arg, at 236,726 to 236,802
_RS01145
BWI76_RS01150: BWI76_RS01150 - tRNA-His, at 236,858 to 236,933
_RS01150
BWI76_RS01155: BWI76_RS01155 - tRNA-Leu, at 236,956 to 237,042
_RS01155
BWI76_RS01160: BWI76_RS01160 - tRNA-Pro, at 237,088 to 237,164
_RS01160
BWI76_RS01165: BWI76_RS01165 - protoheme IX biogenesis protein HemY, at 237,626 to 238,822
_RS01165
BWI76_RS01170: BWI76_RS01170 - uroporphyrinogen-III C-methyltransferase, at 238,826 to 239,995
_RS01170
Position (kb) 237
238
239 Strain fitness (log2 ratio) -2
-1
0
1 at 237.382 kb on - strand at 237.411 kb on + strand at 237.651 kb on + strand at 237.742 kb on - strand at 237.830 kb on + strand, within BWI76_RS01165 at 237.855 kb on + strand, within BWI76_RS01165 at 237.889 kb on - strand, within BWI76_RS01165 at 238.154 kb on + strand, within BWI76_RS01165 at 238.162 kb on - strand, within BWI76_RS01165 at 238.230 kb on + strand, within BWI76_RS01165 at 238.564 kb on + strand, within BWI76_RS01165 at 238.944 kb on - strand, within BWI76_RS01170 at 239.119 kb on - strand, within BWI76_RS01170 at 239.155 kb on + strand, within BWI76_RS01170 at 239.528 kb on + strand, within BWI76_RS01170 at 239.545 kb on + strand, within BWI76_RS01170
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 237,382 - -2.0 237,411 + -1.2 237,651 + -0.8 237,742 - -0.3 237,830 + BWI76_RS01165 0.17 -0.8 237,855 + BWI76_RS01165 0.19 -2.0 237,889 - BWI76_RS01165 0.22 -1.1 238,154 + BWI76_RS01165 0.44 -2.3 238,162 - BWI76_RS01165 0.45 -1.4 238,230 + BWI76_RS01165 0.50 -0.7 238,564 + BWI76_RS01165 0.78 -1.0 238,944 - BWI76_RS01170 0.10 -0.3 239,119 - BWI76_RS01170 0.25 -1.3 239,155 + BWI76_RS01170 0.28 -2.5 239,528 + BWI76_RS01170 0.60 -0.6 239,545 + BWI76_RS01170 0.61 -1.5
Or see this region's nucleotide sequence