Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00815 and BWI76_RS00820 are separated by 56 nucleotides BWI76_RS00820 and BWI76_RS00825 are separated by 205 nucleotides
BWI76_RS00815: BWI76_RS00815 - glycerol dehydrogenase, at 166,891 to 167,994
_RS00815
BWI76_RS00820: BWI76_RS00820 - fructose-6-phosphate aldolase, at 168,051 to 168,713
_RS00820
BWI76_RS00825: BWI76_RS00825 - AraC family transcriptional regulator, at 168,919 to 169,782
_RS00825
Position (kb)
168
169 Strain fitness (log2 ratio)
-1
0
1
2
3 at 167.100 kb on + strand, within BWI76_RS00815 at 167.207 kb on + strand, within BWI76_RS00815 at 167.221 kb on + strand, within BWI76_RS00815 at 167.285 kb on - strand, within BWI76_RS00815 at 167.510 kb on + strand, within BWI76_RS00815 at 167.640 kb on + strand, within BWI76_RS00815 at 167.735 kb on + strand, within BWI76_RS00815 at 167.743 kb on - strand, within BWI76_RS00815 at 167.896 kb on - strand at 168.079 kb on + strand at 168.079 kb on + strand at 168.096 kb on + strand at 168.189 kb on + strand, within BWI76_RS00820 at 168.297 kb on + strand, within BWI76_RS00820 at 168.305 kb on - strand, within BWI76_RS00820 at 168.384 kb on - strand, within BWI76_RS00820 at 168.395 kb on + strand, within BWI76_RS00820 at 168.481 kb on + strand, within BWI76_RS00820 at 168.590 kb on - strand, within BWI76_RS00820 at 168.742 kb on + strand at 168.848 kb on + strand at 168.978 kb on - strand at 169.099 kb on - strand, within BWI76_RS00825 at 169.128 kb on + strand, within BWI76_RS00825 at 169.137 kb on + strand, within BWI76_RS00825 at 169.285 kb on + strand, within BWI76_RS00825 at 169.293 kb on - strand, within BWI76_RS00825 at 169.346 kb on - strand, within BWI76_RS00825 at 169.495 kb on - strand, within BWI76_RS00825 at 169.509 kb on + strand, within BWI76_RS00825 at 169.529 kb on - strand, within BWI76_RS00825 at 169.610 kb on + strand, within BWI76_RS00825 at 169.625 kb on + strand, within BWI76_RS00825
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 167,100 + BWI76_RS00815 0.19 -0.2 167,207 + BWI76_RS00815 0.29 +0.7 167,221 + BWI76_RS00815 0.30 +0.6 167,285 - BWI76_RS00815 0.36 -0.4 167,510 + BWI76_RS00815 0.56 -0.4 167,640 + BWI76_RS00815 0.68 -0.7 167,735 + BWI76_RS00815 0.76 +0.6 167,743 - BWI76_RS00815 0.77 +0.1 167,896 - +0.4 168,079 + -1.1 168,079 + +0.6 168,096 + +1.2 168,189 + BWI76_RS00820 0.21 +2.2 168,297 + BWI76_RS00820 0.37 -0.4 168,305 - BWI76_RS00820 0.38 +0.6 168,384 - BWI76_RS00820 0.50 +0.8 168,395 + BWI76_RS00820 0.52 +0.2 168,481 + BWI76_RS00820 0.65 -1.1 168,590 - BWI76_RS00820 0.81 +0.9 168,742 + +0.6 168,848 + +1.5 168,978 - +0.2 169,099 - BWI76_RS00825 0.21 +0.4 169,128 + BWI76_RS00825 0.24 +0.4 169,137 + BWI76_RS00825 0.25 +0.8 169,285 + BWI76_RS00825 0.42 -0.8 169,293 - BWI76_RS00825 0.43 +1.2 169,346 - BWI76_RS00825 0.49 -0.3 169,495 - BWI76_RS00825 0.67 -0.9 169,509 + BWI76_RS00825 0.68 -0.2 169,529 - BWI76_RS00825 0.71 +2.8 169,610 + BWI76_RS00825 0.80 +2.8 169,625 + BWI76_RS00825 0.82 +1.4
Or see this region's nucleotide sequence