Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26340 and BWI76_RS26345 overlap by 4 nucleotides BWI76_RS26345 and BWI76_RS26350 are separated by 60 nucleotides BWI76_RS26350 and BWI76_RS26355 are separated by 153 nucleotides BWI76_RS26355 and BWI76_RS26360 are separated by 31 nucleotides
BWI76_RS26340: BWI76_RS26340 - branched-chain amino acid ABC transporter permease, at 5,452,093 to 5,453,373
_RS26340
BWI76_RS26345: BWI76_RS26345 - branched-chain amino acid ABC transporter permease LivH, at 5,453,370 to 5,454,296
_RS26345
BWI76_RS26350: BWI76_RS26350 - branched chain amino acid ABC transporter substrate-binding protein, at 5,454,357 to 5,455,466
_RS26350
BWI76_RS26355: BWI76_RS26355 - hypothetical protein, at 5,455,620 to 5,455,855
_RS26355
BWI76_RS26360: BWI76_RS26360 - aspartate 1-decarboxylase autocleavage activator PanM, at 5,455,887 to 5,456,273
_RS26360
Position (kb) 5454
5455
5456 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5453.394 kb on + strand at 5453.556 kb on - strand, within BWI76_RS26345 at 5453.622 kb on - strand, within BWI76_RS26345 at 5453.889 kb on - strand, within BWI76_RS26345 at 5453.955 kb on + strand, within BWI76_RS26345 at 5453.973 kb on - strand, within BWI76_RS26345 at 5454.161 kb on - strand, within BWI76_RS26345 at 5454.243 kb on - strand at 5454.253 kb on - strand at 5454.292 kb on + strand at 5454.292 kb on + strand at 5454.370 kb on + strand at 5454.609 kb on + strand, within BWI76_RS26350 at 5454.639 kb on + strand, within BWI76_RS26350 at 5454.691 kb on + strand, within BWI76_RS26350 at 5454.691 kb on + strand, within BWI76_RS26350 at 5454.691 kb on + strand, within BWI76_RS26350 at 5454.711 kb on + strand, within BWI76_RS26350 at 5454.837 kb on + strand, within BWI76_RS26350 at 5454.926 kb on - strand, within BWI76_RS26350 at 5455.090 kb on - strand, within BWI76_RS26350 at 5455.170 kb on - strand, within BWI76_RS26350 at 5455.229 kb on + strand, within BWI76_RS26350 at 5455.248 kb on + strand, within BWI76_RS26350 at 5455.349 kb on + strand, within BWI76_RS26350 at 5455.492 kb on - strand at 5455.552 kb on + strand at 5455.770 kb on + strand, within BWI76_RS26355 at 5455.770 kb on + strand, within BWI76_RS26355 at 5455.775 kb on + strand, within BWI76_RS26355 at 5455.778 kb on - strand, within BWI76_RS26355 at 5455.783 kb on - strand, within BWI76_RS26355 at 5455.924 kb on - strand at 5455.924 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,453,394 + +0.5 5,453,556 - BWI76_RS26345 0.20 +0.3 5,453,622 - BWI76_RS26345 0.27 +0.8 5,453,889 - BWI76_RS26345 0.56 -0.4 5,453,955 + BWI76_RS26345 0.63 +2.1 5,453,973 - BWI76_RS26345 0.65 +0.7 5,454,161 - BWI76_RS26345 0.85 +1.2 5,454,243 - -0.9 5,454,253 - +1.9 5,454,292 + +1.2 5,454,292 + -0.6 5,454,370 + +0.2 5,454,609 + BWI76_RS26350 0.23 -0.4 5,454,639 + BWI76_RS26350 0.25 +1.2 5,454,691 + BWI76_RS26350 0.30 +0.4 5,454,691 + BWI76_RS26350 0.30 -0.3 5,454,691 + BWI76_RS26350 0.30 +0.1 5,454,711 + BWI76_RS26350 0.32 +1.2 5,454,837 + BWI76_RS26350 0.43 -0.7 5,454,926 - BWI76_RS26350 0.51 +0.1 5,455,090 - BWI76_RS26350 0.66 +2.6 5,455,170 - BWI76_RS26350 0.73 -0.9 5,455,229 + BWI76_RS26350 0.79 +1.6 5,455,248 + BWI76_RS26350 0.80 +0.8 5,455,349 + BWI76_RS26350 0.89 -0.6 5,455,492 - -1.1 5,455,552 + -1.2 5,455,770 + BWI76_RS26355 0.64 -0.3 5,455,770 + BWI76_RS26355 0.64 +0.3 5,455,775 + BWI76_RS26355 0.66 -0.9 5,455,778 - BWI76_RS26355 0.67 -1.9 5,455,783 - BWI76_RS26355 0.69 +0.0 5,455,924 - -0.4 5,455,924 - -0.6
Or see this region's nucleotide sequence