Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25410 and BWI76_RS25415 overlap by 29 nucleotides BWI76_RS25415 and BWI76_RS25420 are separated by 131 nucleotides BWI76_RS25420 and BWI76_RS25425 are separated by 412 nucleotides
BWI76_RS25410: BWI76_RS25410 - GntR family transcriptional regulator with, at 5,281,820 to 5,282,485
_RS25410
BWI76_RS25415: BWI76_RS25415 - GntR family transcriptional regulator, at 5,282,457 to 5,283,101
_RS25415
BWI76_RS25420: BWI76_RS25420 - malate dehydrogenase, at 5,283,233 to 5,284,171
_RS25420
BWI76_RS25425: BWI76_RS25425 - arginine repressor, at 5,284,584 to 5,285,054
_RS25425
Position (kb) 5283
5284
5285 Strain fitness (log2 ratio) -2
-1
0
1 at 5282.301 kb on - strand, within BWI76_RS25410 at 5282.347 kb on - strand, within BWI76_RS25410 at 5282.351 kb on - strand, within BWI76_RS25410 at 5282.365 kb on + strand at 5282.477 kb on - strand at 5282.493 kb on - strand at 5282.875 kb on + strand, within BWI76_RS25415 at 5283.028 kb on - strand, within BWI76_RS25415 at 5283.192 kb on - strand at 5283.383 kb on + strand, within BWI76_RS25420 at 5283.633 kb on + strand, within BWI76_RS25420 at 5283.826 kb on + strand, within BWI76_RS25420 at 5283.844 kb on - strand, within BWI76_RS25420 at 5283.856 kb on - strand, within BWI76_RS25420 at 5284.085 kb on + strand at 5284.092 kb on - strand at 5284.092 kb on - strand at 5284.106 kb on + strand at 5284.278 kb on + strand at 5284.299 kb on - strand at 5284.333 kb on - strand at 5284.335 kb on + strand at 5284.343 kb on - strand at 5284.343 kb on - strand at 5284.380 kb on + strand at 5284.634 kb on + strand, within BWI76_RS25425 at 5284.914 kb on + strand, within BWI76_RS25425 at 5285.025 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,282,301 - BWI76_RS25410 0.72 -0.4 5,282,347 - BWI76_RS25410 0.79 +0.2 5,282,351 - BWI76_RS25410 0.80 +0.3 5,282,365 + +1.2 5,282,477 - +0.2 5,282,493 - -0.4 5,282,875 + BWI76_RS25415 0.65 -0.4 5,283,028 - BWI76_RS25415 0.89 -0.9 5,283,192 - -0.4 5,283,383 + BWI76_RS25420 0.16 -1.1 5,283,633 + BWI76_RS25420 0.43 +0.5 5,283,826 + BWI76_RS25420 0.63 +0.7 5,283,844 - BWI76_RS25420 0.65 +0.3 5,283,856 - BWI76_RS25420 0.66 +1.2 5,284,085 + +0.6 5,284,092 - +1.1 5,284,092 - +0.8 5,284,106 + +0.4 5,284,278 + -0.3 5,284,299 - +1.2 5,284,333 - -0.6 5,284,335 + +0.3 5,284,343 - +0.4 5,284,343 - -0.4 5,284,380 + +0.6 5,284,634 + BWI76_RS25425 0.11 +1.4 5,284,914 + BWI76_RS25425 0.70 +0.2 5,285,025 + -2.4
Or see this region's nucleotide sequence