Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24135 and BWI76_RS24140 are separated by 48 nucleotides BWI76_RS24140 and BWI76_RS24145 are separated by 50 nucleotides BWI76_RS24145 and BWI76_RS24150 overlap by 1 nucleotides
BWI76_RS24135: BWI76_RS24135 - c4-dicarboxylate permease, at 5,032,637 to 5,033,938
_RS24135
BWI76_RS24140: BWI76_RS24140 - hypothetical protein, at 5,033,987 to 5,034,706
_RS24140
BWI76_RS24145: BWI76_RS24145 - hypothetical protein, at 5,034,757 to 5,035,083
_RS24145
BWI76_RS24150: BWI76_RS24150 - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 5,035,083 to 5,035,802
_RS24150
Position (kb) 5033
5034
5035 Strain fitness (log2 ratio) -1
0
1 at 5033.093 kb on - strand, within BWI76_RS24135 at 5033.172 kb on - strand, within BWI76_RS24135 at 5033.606 kb on - strand, within BWI76_RS24135 at 5033.608 kb on + strand, within BWI76_RS24135 at 5033.706 kb on - strand, within BWI76_RS24135 at 5033.746 kb on + strand, within BWI76_RS24135 at 5033.875 kb on + strand at 5033.953 kb on - strand at 5034.022 kb on + strand at 5034.022 kb on + strand at 5034.030 kb on - strand at 5034.030 kb on - strand at 5034.272 kb on - strand, within BWI76_RS24140 at 5034.306 kb on - strand, within BWI76_RS24140 at 5034.306 kb on - strand, within BWI76_RS24140 at 5034.467 kb on - strand, within BWI76_RS24140 at 5034.515 kb on + strand, within BWI76_RS24140 at 5034.631 kb on + strand, within BWI76_RS24140 at 5034.849 kb on - strand, within BWI76_RS24145 at 5035.067 kb on - strand at 5035.127 kb on + strand at 5035.256 kb on + strand, within BWI76_RS24150 at 5035.264 kb on - strand, within BWI76_RS24150 at 5035.336 kb on + strand, within BWI76_RS24150 at 5035.479 kb on - strand, within BWI76_RS24150 at 5035.684 kb on - strand, within BWI76_RS24150
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 5,033,093 - BWI76_RS24135 0.35 +1.2 5,033,172 - BWI76_RS24135 0.41 +0.5 5,033,606 - BWI76_RS24135 0.74 +0.2 5,033,608 + BWI76_RS24135 0.75 +0.6 5,033,706 - BWI76_RS24135 0.82 +0.3 5,033,746 + BWI76_RS24135 0.85 +0.1 5,033,875 + +0.1 5,033,953 - +0.2 5,034,022 + +0.5 5,034,022 + -0.4 5,034,030 - +0.5 5,034,030 - +0.4 5,034,272 - BWI76_RS24140 0.40 +0.8 5,034,306 - BWI76_RS24140 0.44 +0.0 5,034,306 - BWI76_RS24140 0.44 +0.6 5,034,467 - BWI76_RS24140 0.67 -0.8 5,034,515 + BWI76_RS24140 0.73 +1.3 5,034,631 + BWI76_RS24140 0.89 +0.9 5,034,849 - BWI76_RS24145 0.28 +1.2 5,035,067 - +1.0 5,035,127 + +0.4 5,035,256 + BWI76_RS24150 0.24 +0.3 5,035,264 - BWI76_RS24150 0.25 +0.1 5,035,336 + BWI76_RS24150 0.35 +0.2 5,035,479 - BWI76_RS24150 0.55 -0.3 5,035,684 - BWI76_RS24150 0.83 +0.8
Or see this region's nucleotide sequence