Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20860 and BWI76_RS20865 are separated by 582 nucleotides BWI76_RS20865 and BWI76_RS20870 are separated by 3 nucleotides BWI76_RS20870 and BWI76_RS20875 are separated by 27 nucleotides BWI76_RS20875 and BWI76_RS20880 are separated by 232 nucleotides
BWI76_RS20860: BWI76_RS20860 - aminoglycoside/multidrug transporter subunit AcrD, at 4,360,172 to 4,363,285
_RS20860
BWI76_RS20865: BWI76_RS20865 - arsenate reductase, at 4,363,868 to 4,364,221
_RS20865
BWI76_RS20870: BWI76_RS20870 - succinyl-diaminopimelate desuccinylase, at 4,364,225 to 4,365,352
_RS20870
BWI76_RS20875: BWI76_RS20875 - membrane protein, at 4,365,380 to 4,365,583
_RS20875
BWI76_RS20880: BWI76_RS20880 - esterase, at 4,365,816 to 4,366,514
_RS20880
Position (kb) 4364
4365
4366 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4363.242 kb on - strand at 4363.366 kb on + strand at 4363.379 kb on + strand at 4363.379 kb on + strand at 4363.387 kb on - strand at 4363.449 kb on + strand at 4363.451 kb on + strand at 4363.459 kb on + strand at 4363.531 kb on + strand at 4363.539 kb on - strand at 4363.549 kb on - strand at 4363.658 kb on + strand at 4363.670 kb on + strand at 4363.670 kb on + strand at 4363.670 kb on + strand at 4363.738 kb on + strand at 4363.744 kb on - strand at 4363.859 kb on + strand at 4363.859 kb on + strand at 4363.859 kb on + strand at 4363.859 kb on + strand at 4363.860 kb on + strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.867 kb on - strand at 4363.868 kb on + strand at 4363.931 kb on - strand, within BWI76_RS20865 at 4363.987 kb on - strand, within BWI76_RS20865 at 4364.014 kb on + strand, within BWI76_RS20865 at 4364.014 kb on + strand, within BWI76_RS20865 at 4364.016 kb on + strand, within BWI76_RS20865 at 4364.019 kb on + strand, within BWI76_RS20865 at 4364.022 kb on - strand, within BWI76_RS20865 at 4364.027 kb on - strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.029 kb on + strand, within BWI76_RS20865 at 4364.037 kb on - strand, within BWI76_RS20865 at 4364.037 kb on - strand, within BWI76_RS20865 at 4364.038 kb on - strand, within BWI76_RS20865 at 4364.046 kb on - strand, within BWI76_RS20865 at 4364.046 kb on - strand, within BWI76_RS20865 at 4364.092 kb on + strand, within BWI76_RS20865 at 4364.094 kb on - strand, within BWI76_RS20865 at 4364.100 kb on - strand, within BWI76_RS20865 at 4364.144 kb on + strand, within BWI76_RS20865 at 4364.206 kb on + strand at 4365.337 kb on + strand at 4365.407 kb on + strand, within BWI76_RS20875 at 4366.189 kb on - strand, within BWI76_RS20880
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,363,242 - -0.9 4,363,366 + +0.5 4,363,379 + -0.5 4,363,379 + -0.5 4,363,387 - -0.5 4,363,449 + +1.1 4,363,451 + -0.1 4,363,459 + -0.9 4,363,531 + -1.2 4,363,539 - -0.3 4,363,549 - +0.6 4,363,658 + -0.1 4,363,670 + +0.2 4,363,670 + -0.3 4,363,670 + +0.1 4,363,738 + +0.2 4,363,744 - -0.5 4,363,859 + +0.2 4,363,859 + +0.1 4,363,859 + +0.1 4,363,859 + +0.8 4,363,860 + -1.5 4,363,867 - +2.8 4,363,867 - -0.7 4,363,867 - +1.1 4,363,867 - -0.8 4,363,867 - -1.9 4,363,867 - -0.4 4,363,867 - +1.4 4,363,867 - -2.8 4,363,868 + -1.0 4,363,931 - BWI76_RS20865 0.18 -0.9 4,363,987 - BWI76_RS20865 0.34 -0.5 4,364,014 + BWI76_RS20865 0.41 +1.1 4,364,014 + BWI76_RS20865 0.41 -0.9 4,364,016 + BWI76_RS20865 0.42 -0.4 4,364,019 + BWI76_RS20865 0.43 +0.8 4,364,022 - BWI76_RS20865 0.44 -0.0 4,364,027 - BWI76_RS20865 0.45 +0.6 4,364,029 + BWI76_RS20865 0.45 +0.1 4,364,029 + BWI76_RS20865 0.45 +0.2 4,364,029 + BWI76_RS20865 0.45 +0.2 4,364,029 + BWI76_RS20865 0.45 -0.1 4,364,037 - BWI76_RS20865 0.48 -1.2 4,364,037 - BWI76_RS20865 0.48 +1.2 4,364,038 - BWI76_RS20865 0.48 -1.2 4,364,046 - BWI76_RS20865 0.50 +0.3 4,364,046 - BWI76_RS20865 0.50 +0.3 4,364,092 + BWI76_RS20865 0.63 -0.8 4,364,094 - BWI76_RS20865 0.64 +0.7 4,364,100 - BWI76_RS20865 0.66 -0.7 4,364,144 + BWI76_RS20865 0.78 -0.0 4,364,206 + -0.4 4,365,337 + +0.8 4,365,407 + BWI76_RS20875 0.13 +2.8 4,366,189 - BWI76_RS20880 0.53 +0.1
Or see this region's nucleotide sequence