Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19815 and BWI76_RS19820 are separated by 137 nucleotides BWI76_RS19820 and BWI76_RS19825 are separated by 61 nucleotides BWI76_RS19825 and BWI76_RS19830 are separated by 80 nucleotides BWI76_RS19830 and BWI76_RS19835 are separated by 50 nucleotides
BWI76_RS19815: BWI76_RS19815 - ATP-dependent helicase, at 4,143,952 to 4,145,709
_RS19815
BWI76_RS19820: BWI76_RS19820 - 50S ribosomal protein L25, at 4,145,847 to 4,146,131
_RS19820
BWI76_RS19825: BWI76_RS19825 - DNA mismatch repair protein MutT, at 4,146,193 to 4,146,786
_RS19825
BWI76_RS19830: BWI76_RS19830 - DeoR family transcriptional regulator, at 4,146,867 to 4,147,625
_RS19830
BWI76_RS19835: BWI76_RS19835 - nucleoid-associated protein YejK, at 4,147,676 to 4,148,683
_RS19835
Position (kb) 4146
4147 Strain fitness (log2 ratio) -1
0
1 at 4145.306 kb on + strand, within BWI76_RS19815 at 4145.481 kb on + strand, within BWI76_RS19815 at 4145.494 kb on - strand, within BWI76_RS19815 at 4145.504 kb on + strand, within BWI76_RS19815 at 4145.504 kb on + strand, within BWI76_RS19815 at 4145.523 kb on - strand, within BWI76_RS19815 at 4145.534 kb on + strand at 4146.196 kb on + strand at 4146.557 kb on + strand, within BWI76_RS19825 at 4146.665 kb on - strand, within BWI76_RS19825 at 4146.691 kb on + strand, within BWI76_RS19825 at 4147.569 kb on - strand at 4147.625 kb on + strand at 4147.628 kb on - strand at 4147.628 kb on - strand at 4147.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,145,306 + BWI76_RS19815 0.77 +0.0 4,145,481 + BWI76_RS19815 0.87 -0.3 4,145,494 - BWI76_RS19815 0.88 +0.6 4,145,504 + BWI76_RS19815 0.88 -0.1 4,145,504 + BWI76_RS19815 0.88 -1.3 4,145,523 - BWI76_RS19815 0.89 -0.2 4,145,534 + -0.3 4,146,196 + +0.5 4,146,557 + BWI76_RS19825 0.61 +0.6 4,146,665 - BWI76_RS19825 0.79 +0.3 4,146,691 + BWI76_RS19825 0.84 +1.0 4,147,569 - -0.1 4,147,625 + -1.0 4,147,628 - +0.5 4,147,628 - -0.2 4,147,717 + -1.2
Or see this region's nucleotide sequence