Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18195 and BWI76_RS18200 are separated by 100 nucleotides BWI76_RS18200 and BWI76_RS18205 are separated by 76 nucleotides BWI76_RS18205 and BWI76_RS18210 are separated by 230 nucleotides
BWI76_RS18195: BWI76_RS18195 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 3,779,561 to 3,780,577
_RS18195
BWI76_RS18200: BWI76_RS18200 - copper homeostasis protein CutC, at 3,780,678 to 3,781,412
_RS18200
BWI76_RS18205: BWI76_RS18205 - VOC family protein, at 3,781,489 to 3,782,058
_RS18205
BWI76_RS18210: BWI76_RS18210 - arginine--tRNA ligase, at 3,782,289 to 3,784,022
_RS18210
Position (kb) 3780
3781
3782 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3779.775 kb on + strand, within BWI76_RS18195 at 3779.814 kb on + strand, within BWI76_RS18195 at 3780.107 kb on - strand, within BWI76_RS18195 at 3780.317 kb on - strand, within BWI76_RS18195 at 3780.878 kb on + strand, within BWI76_RS18200 at 3780.878 kb on + strand, within BWI76_RS18200 at 3780.886 kb on - strand, within BWI76_RS18200 at 3780.886 kb on - strand, within BWI76_RS18200 at 3780.886 kb on - strand, within BWI76_RS18200 at 3780.886 kb on - strand, within BWI76_RS18200 at 3780.886 kb on - strand, within BWI76_RS18200 at 3780.886 kb on - strand, within BWI76_RS18200 at 3781.070 kb on - strand, within BWI76_RS18200 at 3781.327 kb on + strand, within BWI76_RS18200 at 3781.875 kb on + strand, within BWI76_RS18205 at 3782.146 kb on + strand at 3782.184 kb on - strand at 3782.196 kb on - strand at 3782.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,779,775 + BWI76_RS18195 0.21 +0.9 3,779,814 + BWI76_RS18195 0.25 -1.0 3,780,107 - BWI76_RS18195 0.54 -1.0 3,780,317 - BWI76_RS18195 0.74 -2.0 3,780,878 + BWI76_RS18200 0.27 -0.5 3,780,878 + BWI76_RS18200 0.27 +2.4 3,780,886 - BWI76_RS18200 0.28 +1.5 3,780,886 - BWI76_RS18200 0.28 +1.8 3,780,886 - BWI76_RS18200 0.28 +1.7 3,780,886 - BWI76_RS18200 0.28 +1.1 3,780,886 - BWI76_RS18200 0.28 +0.6 3,780,886 - BWI76_RS18200 0.28 +1.0 3,781,070 - BWI76_RS18200 0.53 +0.3 3,781,327 + BWI76_RS18200 0.88 +0.4 3,781,875 + BWI76_RS18205 0.68 -0.2 3,782,146 + -2.7 3,782,184 - +0.3 3,782,196 - +0.6 3,782,211 + -0.3
Or see this region's nucleotide sequence