Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17075 and BWI76_RS17080 are separated by 117 nucleotides BWI76_RS17080 and BWI76_RS17085 are separated by 34 nucleotides BWI76_RS17085 and BWI76_RS17090 are separated by 126 nucleotides BWI76_RS17090 and BWI76_RS17095 are separated by 81 nucleotides
BWI76_RS17075: BWI76_RS17075 - 3-deoxy-7-phosphoheptulonate synthase, at 3,543,769 to 3,544,815
_RS17075
BWI76_RS17080: BWI76_RS17080 - hypothetical protein, at 3,544,933 to 3,545,160
_RS17080
BWI76_RS17085: BWI76_RS17085 - hypothetical protein, at 3,545,195 to 3,546,637
_RS17085
BWI76_RS17090: BWI76_RS17090 - NLP/P60 protein, at 3,546,764 to 3,547,228
_RS17090
BWI76_RS17095: BWI76_RS17095 - vitamin B12 ABC transporter ATP-binding protein BtuD, at 3,547,310 to 3,548,059
_RS17095
Position (kb) 3545
3546
3547 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 3544.279 kb on - strand, within BWI76_RS17075 at 3544.310 kb on - strand, within BWI76_RS17075 at 3544.444 kb on - strand, within BWI76_RS17075 at 3545.073 kb on - strand, within BWI76_RS17080 at 3545.109 kb on + strand, within BWI76_RS17080 at 3545.112 kb on - strand, within BWI76_RS17080 at 3545.117 kb on - strand, within BWI76_RS17080 at 3545.121 kb on + strand, within BWI76_RS17080 at 3545.122 kb on - strand, within BWI76_RS17080 at 3545.140 kb on + strand at 3545.180 kb on - strand at 3545.385 kb on - strand, within BWI76_RS17085 at 3545.420 kb on - strand, within BWI76_RS17085 at 3545.599 kb on - strand, within BWI76_RS17085 at 3546.020 kb on - strand, within BWI76_RS17085 at 3546.160 kb on + strand, within BWI76_RS17085 at 3546.257 kb on + strand, within BWI76_RS17085 at 3546.374 kb on - strand, within BWI76_RS17085 at 3546.465 kb on + strand, within BWI76_RS17085 at 3546.547 kb on - strand at 3547.496 kb on + strand, within BWI76_RS17095 at 3547.564 kb on + strand, within BWI76_RS17095
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,544,279 - BWI76_RS17075 0.49 -2.0 3,544,310 - BWI76_RS17075 0.52 -0.6 3,544,444 - BWI76_RS17075 0.64 +0.4 3,545,073 - BWI76_RS17080 0.61 +0.3 3,545,109 + BWI76_RS17080 0.77 -1.4 3,545,112 - BWI76_RS17080 0.79 +0.5 3,545,117 - BWI76_RS17080 0.81 -1.1 3,545,121 + BWI76_RS17080 0.82 -0.7 3,545,122 - BWI76_RS17080 0.83 -0.1 3,545,140 + +0.6 3,545,180 - -1.8 3,545,385 - BWI76_RS17085 0.13 +2.9 3,545,420 - BWI76_RS17085 0.16 +0.3 3,545,599 - BWI76_RS17085 0.28 +1.3 3,546,020 - BWI76_RS17085 0.57 -0.2 3,546,160 + BWI76_RS17085 0.67 +1.0 3,546,257 + BWI76_RS17085 0.74 -0.3 3,546,374 - BWI76_RS17085 0.82 +0.8 3,546,465 + BWI76_RS17085 0.88 -1.0 3,546,547 - +1.0 3,547,496 + BWI76_RS17095 0.25 +0.1 3,547,564 + BWI76_RS17095 0.34 +0.1
Or see this region's nucleotide sequence