Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15000 and BWI76_RS15005 are separated by 335 nucleotides BWI76_RS15005 and BWI76_RS15010 are separated by 71 nucleotides BWI76_RS15010 and BWI76_RS15015 are separated by 41 nucleotides BWI76_RS15015 and BWI76_RS15020 are separated by 270 nucleotides
BWI76_RS15000: BWI76_RS15000 - amino acid ABC transporter ATP-binding protein, at 3,142,462 to 3,143,202
_RS15000
BWI76_RS15005: BWI76_RS15005 - hypothetical protein, at 3,143,538 to 3,143,747
_RS15005
BWI76_RS15010: BWI76_RS15010 - hypothetical protein, at 3,143,819 to 3,144,007
_RS15010
BWI76_RS15015: BWI76_RS15015 - DUF2132 domain-containing protein, at 3,144,049 to 3,144,324
_RS15015
BWI76_RS15020: BWI76_RS15020 - 2-oxobutyrate oxidase, at 3,144,595 to 3,145,623
_RS15020
Position (kb)
3143
3144
3145 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3142.847 kb on + strand at 3142.852 kb on - strand, within BWI76_RS15000 at 3142.922 kb on + strand, within BWI76_RS15000 at 3142.995 kb on + strand, within BWI76_RS15000 at 3143.003 kb on - strand at 3143.058 kb on + strand, within BWI76_RS15000 at 3143.058 kb on + strand, within BWI76_RS15000 at 3143.126 kb on + strand, within BWI76_RS15000 at 3143.127 kb on + strand, within BWI76_RS15000 at 3143.135 kb on - strand at 3143.137 kb on + strand at 3143.212 kb on + strand at 3143.245 kb on - strand at 3143.281 kb on + strand at 3143.287 kb on - strand at 3143.362 kb on + strand at 3143.614 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.628 kb on + strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.636 kb on - strand, within BWI76_RS15005 at 3143.670 kb on + strand, within BWI76_RS15005 at 3143.670 kb on + strand, within BWI76_RS15005 at 3143.673 kb on - strand, within BWI76_RS15005 at 3143.678 kb on - strand, within BWI76_RS15005 at 3143.705 kb on + strand, within BWI76_RS15005 at 3143.728 kb on + strand at 3143.728 kb on + strand at 3143.728 kb on + strand at 3143.736 kb on - strand at 3143.736 kb on - strand at 3143.736 kb on - strand at 3143.736 kb on - strand at 3143.838 kb on + strand, within BWI76_RS15010 at 3143.848 kb on + strand, within BWI76_RS15010 at 3143.848 kb on + strand, within BWI76_RS15010 at 3143.848 kb on + strand, within BWI76_RS15010 at 3143.848 kb on + strand, within BWI76_RS15010 at 3143.848 kb on + strand, within BWI76_RS15010 at 3143.864 kb on + strand, within BWI76_RS15010 at 3143.864 kb on + strand, within BWI76_RS15010 at 3143.888 kb on + strand, within BWI76_RS15010 at 3143.888 kb on + strand, within BWI76_RS15010 at 3143.890 kb on + strand, within BWI76_RS15010 at 3143.890 kb on + strand, within BWI76_RS15010 at 3143.890 kb on + strand, within BWI76_RS15010 at 3143.898 kb on - strand, within BWI76_RS15010 at 3143.898 kb on - strand, within BWI76_RS15010 at 3143.905 kb on + strand, within BWI76_RS15010 at 3144.258 kb on - strand, within BWI76_RS15015 at 3144.306 kb on - strand at 3144.329 kb on - strand at 3144.364 kb on - strand at 3144.366 kb on - strand at 3144.395 kb on - strand at 3144.401 kb on - strand at 3144.492 kb on + strand at 3144.500 kb on - strand at 3144.508 kb on - strand at 3144.544 kb on + strand at 3144.730 kb on - strand, within BWI76_RS15020 at 3144.923 kb on - strand, within BWI76_RS15020
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,142,847 + -0.5 3,142,852 - BWI76_RS15000 0.53 -0.5 3,142,922 + BWI76_RS15000 0.62 +0.0 3,142,995 + BWI76_RS15000 0.72 +0.3 3,143,003 - -1.5 3,143,058 + BWI76_RS15000 0.80 -0.9 3,143,058 + BWI76_RS15000 0.80 -0.9 3,143,126 + BWI76_RS15000 0.90 +1.1 3,143,127 + BWI76_RS15000 0.90 -1.2 3,143,135 - -1.2 3,143,137 + +1.0 3,143,212 + -0.5 3,143,245 - -0.3 3,143,281 + +1.3 3,143,287 - +1.4 3,143,362 + +0.1 3,143,614 + BWI76_RS15005 0.36 +3.1 3,143,628 + BWI76_RS15005 0.43 -1.5 3,143,628 + BWI76_RS15005 0.43 -1.4 3,143,628 + BWI76_RS15005 0.43 -1.2 3,143,628 + BWI76_RS15005 0.43 +0.1 3,143,628 + BWI76_RS15005 0.43 +1.1 3,143,628 + BWI76_RS15005 0.43 -1.2 3,143,628 + BWI76_RS15005 0.43 +1.4 3,143,628 + BWI76_RS15005 0.43 -0.9 3,143,628 + BWI76_RS15005 0.43 +1.8 3,143,628 + BWI76_RS15005 0.43 -0.5 3,143,628 + BWI76_RS15005 0.43 +0.1 3,143,636 - BWI76_RS15005 0.47 +0.1 3,143,636 - BWI76_RS15005 0.47 +0.1 3,143,636 - BWI76_RS15005 0.47 +0.0 3,143,636 - BWI76_RS15005 0.47 -0.9 3,143,636 - BWI76_RS15005 0.47 -1.2 3,143,636 - BWI76_RS15005 0.47 +0.1 3,143,636 - BWI76_RS15005 0.47 +0.5 3,143,636 - BWI76_RS15005 0.47 -1.7 3,143,636 - BWI76_RS15005 0.47 +0.1 3,143,636 - BWI76_RS15005 0.47 -1.5 3,143,636 - BWI76_RS15005 0.47 -0.2 3,143,636 - BWI76_RS15005 0.47 -0.3 3,143,636 - BWI76_RS15005 0.47 -1.2 3,143,636 - BWI76_RS15005 0.47 -0.5 3,143,636 - BWI76_RS15005 0.47 -1.1 3,143,670 + BWI76_RS15005 0.63 -0.5 3,143,670 + BWI76_RS15005 0.63 -0.2 3,143,673 - BWI76_RS15005 0.64 -0.5 3,143,678 - BWI76_RS15005 0.67 +0.2 3,143,705 + BWI76_RS15005 0.80 -0.6 3,143,728 + +1.4 3,143,728 + +0.0 3,143,728 + +1.1 3,143,736 - +1.1 3,143,736 - +0.0 3,143,736 - -3.2 3,143,736 - +0.8 3,143,838 + BWI76_RS15010 0.10 +0.1 3,143,848 + BWI76_RS15010 0.15 -0.5 3,143,848 + BWI76_RS15010 0.15 -2.3 3,143,848 + BWI76_RS15010 0.15 +0.5 3,143,848 + BWI76_RS15010 0.15 +0.2 3,143,848 + BWI76_RS15010 0.15 -3.4 3,143,864 + BWI76_RS15010 0.24 +2.1 3,143,864 + BWI76_RS15010 0.24 +0.1 3,143,888 + BWI76_RS15010 0.37 -0.4 3,143,888 + BWI76_RS15010 0.37 -0.2 3,143,890 + BWI76_RS15010 0.38 -0.2 3,143,890 + BWI76_RS15010 0.38 -0.3 3,143,890 + BWI76_RS15010 0.38 -2.7 3,143,898 - BWI76_RS15010 0.42 +0.0 3,143,898 - BWI76_RS15010 0.42 -0.7 3,143,905 + BWI76_RS15010 0.46 -0.5 3,144,258 - BWI76_RS15015 0.76 +0.7 3,144,306 - +2.0 3,144,329 - -0.7 3,144,364 - -0.1 3,144,366 - -1.2 3,144,395 - -1.8 3,144,401 - -0.5 3,144,492 + -0.3 3,144,500 - +0.1 3,144,508 - -0.2 3,144,544 + +0.5 3,144,730 - BWI76_RS15020 0.13 -1.7 3,144,923 - BWI76_RS15020 0.32 -2.1
Or see this region's nucleotide sequence