Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14380 and BWI76_RS14385 overlap by 4 nucleotides BWI76_RS14385 and BWI76_RS14390 overlap by 17 nucleotides
BWI76_RS14380: BWI76_RS14380 - hypothetical protein, at 3,003,090 to 3,005,456
_RS14380
BWI76_RS14385: BWI76_RS14385 - hypothetical protein, at 3,005,453 to 3,007,003
_RS14385
BWI76_RS14390: BWI76_RS14390 - hypothetical protein, at 3,006,987 to 3,007,862
_RS14390
Position (kb)
3005
3006
3007
3008 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3004.475 kb on - strand, within BWI76_RS14380 at 3004.537 kb on + strand, within BWI76_RS14380 at 3004.552 kb on - strand, within BWI76_RS14380 at 3004.552 kb on - strand, within BWI76_RS14380 at 3004.552 kb on - strand, within BWI76_RS14380 at 3004.851 kb on - strand, within BWI76_RS14380 at 3004.851 kb on - strand, within BWI76_RS14380 at 3004.908 kb on - strand at 3004.952 kb on + strand at 3005.041 kb on + strand at 3005.049 kb on - strand, within BWI76_RS14380 at 3005.049 kb on - strand, within BWI76_RS14380 at 3005.153 kb on - strand, within BWI76_RS14380 at 3005.208 kb on + strand, within BWI76_RS14380 at 3005.211 kb on - strand, within BWI76_RS14380 at 3005.248 kb on + strand at 3005.362 kb on + strand at 3005.362 kb on + strand at 3005.370 kb on - strand at 3005.557 kb on + strand at 3005.565 kb on - strand at 3005.609 kb on + strand, within BWI76_RS14385 at 3005.609 kb on + strand, within BWI76_RS14385 at 3005.617 kb on - strand at 3005.627 kb on - strand, within BWI76_RS14385 at 3005.646 kb on + strand, within BWI76_RS14385 at 3005.819 kb on - strand, within BWI76_RS14385 at 3005.998 kb on + strand, within BWI76_RS14385 at 3006.006 kb on - strand, within BWI76_RS14385 at 3006.146 kb on - strand, within BWI76_RS14385 at 3006.164 kb on + strand at 3006.242 kb on + strand, within BWI76_RS14385 at 3006.350 kb on - strand, within BWI76_RS14385 at 3006.356 kb on - strand, within BWI76_RS14385 at 3006.366 kb on - strand, within BWI76_RS14385 at 3006.403 kb on - strand, within BWI76_RS14385 at 3006.480 kb on - strand, within BWI76_RS14385 at 3006.571 kb on + strand, within BWI76_RS14385 at 3006.658 kb on + strand, within BWI76_RS14385 at 3006.792 kb on + strand, within BWI76_RS14385 at 3006.799 kb on - strand, within BWI76_RS14385 at 3007.025 kb on + strand at 3007.224 kb on - strand at 3007.224 kb on - strand, within BWI76_RS14390 at 3007.224 kb on - strand, within BWI76_RS14390 at 3007.860 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,004,475 - BWI76_RS14380 0.59 -0.5 3,004,537 + BWI76_RS14380 0.61 +0.3 3,004,552 - BWI76_RS14380 0.62 +0.2 3,004,552 - BWI76_RS14380 0.62 +1.1 3,004,552 - BWI76_RS14380 0.62 +1.2 3,004,851 - BWI76_RS14380 0.74 +0.1 3,004,851 - BWI76_RS14380 0.74 +0.2 3,004,908 - -0.5 3,004,952 + +0.1 3,005,041 + -0.5 3,005,049 - BWI76_RS14380 0.83 -0.5 3,005,049 - BWI76_RS14380 0.83 +0.4 3,005,153 - BWI76_RS14380 0.87 +0.5 3,005,208 + BWI76_RS14380 0.89 -0.9 3,005,211 - BWI76_RS14380 0.90 +0.1 3,005,248 + +1.5 3,005,362 + +0.2 3,005,362 + +0.8 3,005,370 - +0.6 3,005,557 + +0.2 3,005,565 - +0.2 3,005,609 + BWI76_RS14385 0.10 -0.8 3,005,609 + BWI76_RS14385 0.10 -1.9 3,005,617 - +1.1 3,005,627 - BWI76_RS14385 0.11 -1.4 3,005,646 + BWI76_RS14385 0.12 +1.1 3,005,819 - BWI76_RS14385 0.24 +0.1 3,005,998 + BWI76_RS14385 0.35 -0.9 3,006,006 - BWI76_RS14385 0.36 -0.9 3,006,146 - BWI76_RS14385 0.45 -1.5 3,006,164 + +1.1 3,006,242 + BWI76_RS14385 0.51 -1.2 3,006,350 - BWI76_RS14385 0.58 -1.8 3,006,356 - BWI76_RS14385 0.58 +0.4 3,006,366 - BWI76_RS14385 0.59 -0.0 3,006,403 - BWI76_RS14385 0.61 -0.8 3,006,480 - BWI76_RS14385 0.66 +1.4 3,006,571 + BWI76_RS14385 0.72 -0.2 3,006,658 + BWI76_RS14385 0.78 -0.8 3,006,792 + BWI76_RS14385 0.86 -0.5 3,006,799 - BWI76_RS14385 0.87 +2.1 3,007,025 + -0.5 3,007,224 - -0.5 3,007,224 - BWI76_RS14390 0.27 -3.2 3,007,224 - BWI76_RS14390 0.27 -0.5 3,007,860 + +1.1
Or see this region's nucleotide sequence